Shedding of Immature Germ Cells

Ariagno, Julia Irene; Curi, Susana Mercedes; Mendeluk, Gabriela Ruth; Grinspon, Diana; Repetto, Herberto Ernesto; Chenlo, Patricia Haydee; Pugliese, N; Sardi, M; Blanco, A M

doi:10.1080/014850102317267436

Cited by 11 publications

(10 citation statements)

References 10 publications

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“…The studies by Fedder et al .,[3] Gandini et al .,[1] and Ariagno et al .,[11] reported similar values. When the total count and occurrence of germ cells was correlated it showed a negative correlation.…”

Section: Discussionsupporting

confidence: 56%

Immature germ cells in semen - correlation with total sperm count and sperm motility

Patil

Humbarwadi

Patil

et al. 2013

J Cytol

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Background:Current data regarding infertility suggests that male factor contributes up to 30% of the total cases of infertility. Semen analysis reveals the presence of spermatozoa as well as a number of non-sperm cells, presently being mentioned in routine semen report as “round cells” without further differentiating them into leucocytes or immature germ cells.Aim:The aim of this work was to study a simple, cost-effective, and convenient method for differentiating the round cells in semen into immature germ cells and leucocytes and correlating them with total sperm counts and motility.Materials and Methods:Semen samples from 120 males, who had come for investigation for infertility, were collected, semen parameters recorded, and stained smears studied for different round cells. Statistical analysis of the data was done to correlate total sperm counts and sperm motility with the occurrence of immature germ cells and leucocytes. The average shedding of immature germ cells in different groups with normal and low sperm counts was compared. The clinical significance of “round cells” in semen and their differentiation into leucocytes and immature germ cells are discussed.Conclusions:Round cells in semen can be differentiated into immature germ cells and leucocytes using simple staining methods. The differential counts mentioned in a semen report give valuable and clinically relevant information. In this study, we observed a negative correlation between total count and immature germ cells, as well as sperm motility and shedding of immature germ cells. The latter was statistically significant with a P value 0.000.

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Section: Discussionsupporting

confidence: 56%

Immature germ cells in semen - correlation with total sperm count and sperm motility

Patil

Humbarwadi

Patil

et al. 2013

J Cytol

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“…The presence of immature sperm cells reveals a dysfunction of spermatogenesis [5]. In pathological semen, the dynamic of spermiation reflected by the ratio between immature sperm cells and cells stemming from testes is altered [4,14]. We found this parameter markedly higher in the combined asthenozoospermia.…”

Section: Discussionmentioning

confidence: 47%

“…Both data were employed to quantify the shedding of GC (reference value < 1.7 Â 10 6 =mL) [5]. Results were expressed as the rate of the number of GC versus number of GC þ total sperm count (GC vs. Sp þ GC) (reference value < 2.5%) [4].…”

Section: Methodsmentioning

confidence: 99%

Asthenozoospermia: Analysis of a Large Population

Curi

Ariagno

Chenlo

et al. 2003

Archives of Andrology

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208

142

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“…In addition to the role of sperm density, morphology, and vitality to evaluate testicular function, analysis of sperm precursors (i.e., spermatogonia, primary and secondary spermatocytes, and spermatids) would be desirable for infertile men. Abnormal exfoliation of immature germ cells (IGC) is occasionally observed in SA as a result of seminiferous tubules dysfunction, hypospermatogenesis, varicocele, and other testicular disturbances, all related to male infertility (37)(38)(39)(40).…”

Section: Sperm Precursorsmentioning

confidence: 99%

Semen analysis in laboratory practice: An overview of routine tests

Andrade-Rocha¹

2003

Clinical Laboratory Analysis

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Semen analysis is a basic step in the investigation of several disturbances affecting the male genital tract. Analysis of seminal parameters provides important clinical information on the spermatogenesis and functional competence of spermatozoa, as well as on the secretory pattern of the accessory genital glands. Semen analysis is particularly useful in the evaluation of couples requiring fertility investigation (to detect genital infections and pathologies) and in verifying the influence of environmental factors, drugs, lifestyle, chemical products, and professional activities on several diseases affecting male reproductive health. Measure of semen quality is of substantial interest for diagnoses in clinical urology, andrology, and gynecology. Currently, basic requirements for semen analysis are standardized by World Health Organization (WHO) guidelines that describe several procedures for an objective evaluation of the semen quality with diagnostic purposes. These guidelines include: parameters for the physical and biochemical evaluation of semen; parameters for the analysis of sperm characteristics; and other seminal parameters that can be easily adopted in any laboratory. This report summarizes current concepts on semen analysis and the significance of the seminal parameters for reaching a diagnosis based on the procedures recommended by WHO guidelines.

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Shedding of Immature Germ Cells

Cited by 11 publications

References 10 publications

Immature germ cells in semen - correlation with total sperm count and sperm motility

Immature germ cells in semen - correlation with total sperm count and sperm motility

Asthenozoospermia: Analysis of a Large Population

Semen analysis in laboratory practice: An overview of routine tests

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