Sheathless Capillary Electrophoresis&amp;#8211;Mass Spectrometry for Metabolic Profiling of Biological Samples

Zhang, Wei; Gulersonmez, M. Can; Hankemeier, Thomas; Ramautar, Rawi

doi:10.3791/54535

Cited by 13 publications

(11 citation statements)

References 21 publications

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“…New capillaries were conditioned and rinsing procedures were performed between runs as previously described. [ 56 ] All samples were hydrodynamically injected (at 2 psi for 30 s for cations and 40 s for anions, corresponding to 1.07% of the capillary volume or ≈6.89 nL and 1.43% and 9.19 nL, respectively) and separated with 10% acetic acid (pH 2.2) as the background electrolyte (BGE). A voltage of 30 kV was used for electrophoretic separation.…”

Section: Methodsmentioning

confidence: 99%

The Nanomaterial Metabolite Corona Determined Using a Quantitative Metabolomics Approach: A Pilot Study

Chetwynd

Zhang

Thorn³

et al. 2020

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Nanomaterials (NMs) are promptly coated with biomolecules in biological systems leading to the formation of the so‐called corona. To date, research has predominantly focused on the protein corona and how it affects NM uptake, distribution, and bioactivity by conferring a biological identity to NMs enabling interactions with receptors to mediate cellular responses. Thus, protein corona studies are now integral to nanosafety assessment. However, a larger class of molecules, the metabolites, which are orders of magnitude smaller than proteins (<1000 Da) and regulate metabolic pathways, has been largely overlooked. This hampers the understanding of the bio–nano interface, development of computational predictions of corona formation, and investigations into uptake or toxicity at the cellular level, including identification of molecular initiating events triggering adverse outcome pathways. Here, a capillary electrophoresis–mass spectrometry based metabolomics approach reveals that pure polar ionogenic metabolite standards differentially adsorb to a range of 6 NMs (SiO2, 3 TiO2 with different surface chemistries, and naïve and carboxylated polystyrene NMs). The metabolite corona composition is quantitatively compared using protein‐free and complete plasma samples, revealing that proteins in samples significantly change the composition of the metabolite corona. This key finding provides the basis to include the metabolite corona in future nanosafety endeavors.

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Section: Methodsmentioning

confidence: 99%

The Nanomaterial Metabolite Corona Determined Using a Quantitative Metabolomics Approach: A Pilot Study

Chetwynd

Zhang

Thorn³

et al. 2020

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“…It can be used for the profiling of cationic metabolites (including AAs, nucleosides and small peptides) or anionic metabolites in various biological samples (urine, cerebrospinal fluid and glioblastoma cell line extracts) .…”

Section: Biomedical Analysesmentioning

confidence: 99%

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

et al. 2017

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Recent advances in amino acid analysis by capillary electromigration methods: June 2015-May 2017In the tenth edition of this article focused on recent advances in amino acid analysis using capillary electrophoresis, we describe the most important research articles published on this topic during the period from June 2015 to May 2017. This article follows the format of the previous articles published in Electrophoresis. The new developments in amino acid analysis with CE mainly describe improvements in CE associated with mass spectrometry. Focusing on applications, we mostly describe clinical works, although metabolomics studies are also very important. Finally, works focusing on amino acids in food and agricultural applications are also described.

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“…Such data is urgently needed to actually show the suitability of CE–MS for long‐term anionic metabolic profiling. The availability of open access peer‐reviewed protocols would be very helpful for this purpose, and in that context the developments are going into the right direction .…”

Section: Discussionmentioning

confidence: 99%

CE–MS for anionic metabolic profiling: An overview of methodological developments

2019

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The efficient profiling of highly polar and charged metabolites in biological samples remains a huge analytical challenge in metabolomics. Over the last decade, new analytical techniques have been developed for the selective and sensitive analysis of polar ionogenic compounds in various matrices. Still, the analysis of such compounds, notably for acidic ionogenic metabolites, remains a challenging endeavor, even more when the available sample size becomes an issue for the total analytical workflow. In this paper, we give an overview of the possibilities of capillary electrophoresis‐mass spectrometry (CE–MS) for anionic metabolic profiling by focusing on main methodological developments. Attention is paid to the development of improved separation conditions and new interfacing designs in CE–MS for anionic metabolic profiling. A complete overview of all CE–MS‐based methods developed for this purpose is provided in table format (Table 1) which includes information on sample type, separation conditions, mass analyzer and limits of detection (LODs). Selected applications are discussed to show the utility of CE–MS for anionic metabolic profiling, especially for small‐volume biological samples. On the basis of the examination of the reported literature in this specific field, we conclude that there is still room for the design of a highly sensitive and reliable CE–MS method for anionic metabolic profiling. A rigorous validation and the availability of standard operating procedures would be highly favorable in order to make CE–MS an alternative, viable analytical technique for metabolomics.

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Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Cited by 13 publications

References 21 publications

The Nanomaterial Metabolite Corona Determined Using a Quantitative Metabolomics Approach: A Pilot Study

The Nanomaterial Metabolite Corona Determined Using a Quantitative Metabolomics Approach: A Pilot Study

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

CE–MS for anionic metabolic profiling: An overview of methodological developments

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