Sharing of Individual Antigenic Determinants Between a  G and a  M Protein in the Same Myeloma Serum

Penn, Gerald; Kunkel, H. G.; Grey, Howard M.

doi:10.3181/00379727-135-35116

Cited by 49 publications

(16 citation statements)

References 5 publications

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“…The most compelling evidence for this is derived from the study of sera from myeloma patients, which contain monoclonal IgM and monoclonal IgG2 proteins identical in the heavy chain variable region by partial sequencing and idiotype (5). The sharing of idiotypic markers by IgM and IgG immunoglobulin has been reported in other systems (7,8), and recently several investigators have demonstrated idiotypic cross reactions between a mouse myeloma protein of the IgA class with specificity for phosphocholine (PC) and anti-PC antibody of the IgM class from Balb/c mice (9,10 (15). Limiting doses of spleen cells from unimmunized donor Balb/c mice were injected intravenously into lethally irradiated hemocyanin-primed syngeneic recipients.…”

mentioning

confidence: 99%

Production of antibodies of identical idiotype but diverse immunoglobulin classes by cells derived from a single stimulated B cell.

Gearhart¹,

Sigal²,

Klinman³

1975

Proc. Natl. Acad. Sci. U.S.A.

126

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The availability of anti-phosphocholine antibody of the TEPC 15 idiotype from the clonal progeny of a single precursor cell, stimulated in vitro, permitted the demonstration of monoclonal antibodies with as many as three immunoglobulin classes with identical variable regions. This demonstration was dependent on sensitive radioimmunoassays which showed a one to one relationship between the total anti-phosphocholine antibody produced by a clone and the sum of anti-phosphocholine antibody of the different classes as well as the amount of antibody of the TEPC 15 idiotype. The class distribution was confirmed by isoelectric focusing identification of IgM, IgA, and IgGI antibodies of the TEPC 15 idiotype produced by single clones which showed characteristic pl values for each immunoglobulin class. Thus, within the generative phase of a single antibody-producing cell clone, various heavy chain constant regions can be linked to the same variable region, and single precursor cells have the capacity to generate progeny expressing at least three distinct immunoglobulin classes.

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mentioning

confidence: 99%

Production of antibodies of identical idiotype but diverse immunoglobulin classes by cells derived from a single stimulated B cell.

Gearhart¹,

Sigal²,

Klinman³

1975

Proc. Natl. Acad. Sci. U.S.A.

126

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“…On the other hand, the existence of typical diffuse plasmocytoma with IgM paraproteinemia has been demonstrated [1,2,11,13,16], IgG and IgM double paraproteinemia has been recognized in 1 case of diffuse plas mocytoma [10], in liver solitary plasmocytoma [12] and in plasma cell leukemia [6]. IgM paraproteinemia and IgG heavy chains have been found in 1 case of plasma cell leukemia [5], All these cases, plus the ex perimental evidence in BALB mice [3,7], show that neoplastic plasma cells can synthesize IgM as well as the other types of immunoglobulins.…”

Section: Discussionmentioning

confidence: 99%

Plasmocytoma with IgM Paraproteinemia

Fagiolo¹

1976

Acta Haematol

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A case of typical diffuse plasmocytoma with IgM paraproteinemia is described. The patient presented with systemic and monomorphic proliferation of atypical and immature plasma cells, osteolytic lesions, retinal venous thrombosis and hemorrhagic diathesis. The therapy with steroids and alkeran produced clinical and hematological remission for 1¹/₂ year with a decrease of macroglobulins in the serum. Cell and paraprotein type remained unchanged during the course of the disease. This case, and similar ones already reported in the literature, suggest that neoplastic plasma cell proliferation is accompanied by synthesis of paraproteins including the IgM type. However, this is not characteristic for Waldenström’s macroglobulinemia. The author concludes that differential diagnosis in these cases can only be made by cytologic examination.

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“…This evidence included ( I ) demonstration that different 1. 1 and y-chains share variable sequence subgroups [3]; (11) two plasmacytomas of one patient, having different H-chains still have identical VH region sequences [21]; (111) rabbit p , y, and a-chains share the V region allotypes [4, 51; and (1V) rabbit anti-Salmonella antibodies of the IgG and 1gM classes share idiotypic determinants [6].…”

Section: Discussionmentioning

confidence: 99%

Expression of a mouse Ig V gene in antibodies of three immunoglobulin classes

et al. 1975

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In an earlier study we found that some mouse strains, including the C57BL/6, produced irregular IgG anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies to protein conjugates of this hapten. This antibody had heteroclitic fine specificity and a characteristic isoelectric focusing pattern. Breeding studies suggested that these qualities of the C57BL/6 anti-NP were controlled by a V gene of the heavy chain. We have now found that this V gene is expressed in anti-NP antibodies and plaque-forming cells of all major immunoglobulin classes: IgG, IgA and IgM.

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Sharing of Individual Antigenic Determinants Between a G and a M Protein in the Same Myeloma Serum

Cited by 49 publications

References 5 publications

Production of antibodies of identical idiotype but diverse immunoglobulin classes by cells derived from a single stimulated B cell.

Production of antibodies of identical idiotype but diverse immunoglobulin classes by cells derived from a single stimulated B cell.

Plasmocytoma with IgM Paraproteinemia

Expression of a mouse Ig V gene in antibodies of three immunoglobulin classes

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