Expression of a mouse Ig V gene in antibodies of three immunoglobulin classes

Imanishi, Thereza; Hurme, Mikko; Sarvas, Heikki; Mãkelä, O.

doi:10.1002/eji.1830050309

Cited by 8 publications

(3 citation statements)

References 15 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A longstanding and extensive body of literature shows that antibody pI mainly reflects paratope biochemistry and epitope specificity (13,16,19,35,44,49,57). This fact is evident within a collection of anti-gp120 MAbs that have identical IgG1 Fc portions (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Signature Biochemical Properties of Broadly Cross-Reactive HIV-1 Neutralizing Antibodies in Human Plasma

Sajadi

Lewis

Seaman

et al. 2012

J Virol

View full text Add to dashboard Cite

The common properties of broadly cross-reactive HIV-1 neutralization antibodies found in certain HIV-1-infected individuals holds significant value for understanding natural and vaccine-mediated anti-HIV immunity. Recent efforts have addressed this question by deriving neutralizing monoclonal anti-envelope antibodies from memory B cell pools of selected subjects. However, it has been more difficult to identify whether broadly neutralizing antibodies circulating in plasma possess shared characteristics among individuals. To address this question, we used affinity chromatography and isoelectric focusing to fractionate plasma immunoglobulin from 10 HIV-1-infected subjects (5 subjects with broad HIV-1 neutralizing activity and 5 controls). We find that plasma neutralizing activity typically partitions into at least two subsets of antibodies. Antibodies with restricted neutralization breadth have relatively neutral isoelectric points and preferentially bind to envelope monomers and trimers versus core antigens from which variable loops and other domains have been deleted. In comparison, broadly neutralizing antibodies account for a minor fraction of the total anti-envelope response. They are consistently distinguished by more basic isoelectric points and specificity for epitopes shared by monomeric gp120, gp120 core, or CD4-induced structures. Such biochemical properties might be exploited to reliably predict or produce broad anti-HIV immunity.

show abstract

Section: Discussionmentioning

confidence: 99%

Signature Biochemical Properties of Broadly Cross-Reactive HIV-1 Neutralizing Antibodies in Human Plasma

Sajadi

Lewis

Seaman

et al. 2012

J Virol

View full text Add to dashboard Cite

show abstract

“…Indeed shared Id among IgM, IgA, IgG and IgE antibodies were reported in several experimental systems [13,15,32,33]. It has also been demonstrated that aId obtained from antibodies of one Ig class can suppress or enhance an immune response in other classes against the respective antigen [13,14, …”

Section: Discussionmentioning

confidence: 99%

Regulatory effects of isologous anti‐idiotypic antibodies on the formation of different immunoglobulin classes in the immune response to phosphorylcholine in BALB/c mice

Blaser

Heusser

1984

Eur J Immunol

View full text Add to dashboard Cite

BALB/c mice were immunized with purified phosphorylcholine (PC)-specific myeloma protein of the TEPC-15 tumor, which bears the major idiotype of the anti-PC response (T15 Id) in this mouse strain. Four months after establishing the anti-idiotypic response, animals were immunized with PC-keyhole limpet hemocyanin under conditions which normally lead to IgE antibody formation. The expression of anti-PC antibodies of each immunoglobulin class was compared to a group of matched control mice not immunized with T15. In BALB/c mice producing anti-idiotypic antibodies the formation of anti-PC IgM, IgG1, IgG2, IgG3 and IgE was suppressed to various extents and for different lengths of time. An exception to this observation was the formation of anti-PC IgA antibodies, in that no significant difference was measured between the two groups of mice. BALB/c mice immunized in the same way with the PC-specific myeloma proteins of the MOPC-167, MOPC-511 and MOPC-603 tumors produced normal levels of anti-PC IgE and IgG antibodies. These results suggest that T15 idiotype-positive antibodies are probably formed within all classes of specific antibodies expressed during an immune response to PC. The possibility of extending the phenomena of anti-idiotype-induced suppression to the level of various classes of specific antibodies, including IgE, might be of interest in the treatment of some allergic diseases.

show abstract

“…When the 19S antibodies were obtained from NIP-primed NPboosted mice, the affinity for the boosting hapten, NP-cap, was lower than for NIP-cap, The relative affinities varied from 3.7 In the 2-month challenge inter\'al group to 21 in the 12-month group. We did not have enough 19S secondary response antibody from NIP-NIP or NP-NP immunizations to be tested bj the HPI method, but the specificities of direct plaque-forming cells in NP-NP immunization have been tested in this laboratory by Imanishi et al (16). At that time the relative affinity was 0.37-that is, less than 10^ of the values in the NIP-primed groups.…”

Section: Pine-specificity Of 19s Antibodiesmentioning

confidence: 99%

The Role of B‐Cell Memory in Secondary IgG and IgM Responses

et al. 1976

Self Cite

View full text Add to dashboard Cite

Mice were primed with the hapten 3-nitro-4-hydroxy-5 iodophenacetic acid (NIP) conjugated to chicken globulin (cg) and were boosted 2, 6, or 12 months later with CG conjugates of the related haptens 3,5-diiodo-4-hydroxyphenacetic acid (DIP) or 3-nitro-4-hydroxphenacetic acid (NP). Accelerated secondary responses were demonstrated both in the 7S and 19S class. Fine-specificities of secondary-response antibodies were studied by the hapten inhibition method of haptenated bacteriophage inactivation. 7S antibodies were found to have the fine-specificity of anti-NIP antibodies regardless of whether DIP or NP was the booster hapten ('original antigenic sin'). 19S antibodies had the fine-specificity of anti-DIP when DIP was the booster hapten. NP as the booster hapten resulted in 19S antibodies whose fine-specificity was intermediate between anti-NIP and anti-NP. A strong B-cell memory could thus be demonstrated in the 7S antibody response and a weak B-cell memory in the 19S antibody response.

show abstract

Expression of a mouse Ig V gene in antibodies of three immunoglobulin classes

Cited by 8 publications

References 15 publications

Signature Biochemical Properties of Broadly Cross-Reactive HIV-1 Neutralizing Antibodies in Human Plasma

Signature Biochemical Properties of Broadly Cross-Reactive HIV-1 Neutralizing Antibodies in Human Plasma

Regulatory effects of isologous anti‐idiotypic antibodies on the formation of different immunoglobulin classes in the immune response to phosphorylcholine in BALB/c mice

The Role of B‐Cell Memory in Secondary IgG and IgM Responses

Contact Info

Product

Resources

About