Shared signatures and divergence in skin microbiomes of children with atopic dermatitis and their caregivers

Sharing is caring? Skin microbiome insights into staphylococci in patients with atopic dermatitis and caregivers

“…Consistent with other human microbiome studies, in the study by Chia et al, 7 members of the same household had similar skin microbiota. In comparison with other studies on S aureus culture-positive rates in patients with AD and in general population cohorts, S aureus culture positivity was relatively low in this cohort of patients with AD (55% [n 5 10 of 18] of those with lesional skin and 57% [n 5 17 of 30] of those with nonlesional skin) and relatively high for control households (40%).…”

“…Prior shotgun metagenomics studies investigating staphylococcal strains in patients with AD showed that the predominant S aureus strains were often clonal whereas S epidermidis strains were much more diverse. 2,3 In this issue of the Journal of Allergy and Clinical Immunology, Chia et al 7 have built on their prior AD microbiome work to investigate skin microbiomes in children with AD and their healthy primary caregivers. Chia et al 7 have used shotgun metagenomics and whole genome sequencing of cultivated S aureus isolates from pairs of Singaporean patients with AD and their caregivers (n 5 30) and control-caregiver pairs (n 5 30) to demonstrate that individuals from households containing 1 or more members with AD had skin microbiomes different from those in control households (Fig 1).…”

“…2,3 In this issue of the Journal of Allergy and Clinical Immunology, Chia et al 7 have built on their prior AD microbiome work to investigate skin microbiomes in children with AD and their healthy primary caregivers. Chia et al 7 have used shotgun metagenomics and whole genome sequencing of cultivated S aureus isolates from pairs of Singaporean patients with AD and their caregivers (n 5 30) and control-caregiver pairs (n 5 30) to demonstrate that individuals from households containing 1 or more members with AD had skin microbiomes different from those in control households (Fig 1). Chia et al 7 have examined the ratio of S aureus to Staphylococcus hominis, which distinguished households containing 1 or more members with AD from control households (area under the curve > 0.80; fair to good).…”

“…Chia et al 7 have used shotgun metagenomics and whole genome sequencing of cultivated S aureus isolates from pairs of Singaporean patients with AD and their caregivers (n 5 30) and control-caregiver pairs (n 5 30) to demonstrate that individuals from households containing 1 or more members with AD had skin microbiomes different from those in control households (Fig 1). Chia et al 7 have examined the ratio of S aureus to Staphylococcus hominis, which distinguished households containing 1 or more members with AD from control households (area under the curve > 0.80; fair to good). Interestingly, the stronger correlation between severity based on Scoring Atopic Dermatitis (SCORAD) score and an S aureus-to S-hominis ratio in contrast to normalized relative abundances of S aureus raises the concept that looking beyond S aureus may be important in AD.…”

No evidence for a common blood microbiome based on a population study of 9,770 healthy humans

“…After application of the presence/absence filter, we identified and removed putative contaminants using established decontamination heuristics 26 that have been validated in previous studies 27, 28 , prior to our downstream analyses. These rules were applied using eight types of batch information: source cohort, DNA extraction kit type, library preparation kit type, and lot numbers for sequencing-by-synthesis kit (box 1, box 2), paired-end cluster kit (box 1, box 2) and sequencing flow cell used.…”