Shape of ageing erythrocytes

Cj, Van Oss

doi:10.3233/bir-1982-19606

Cited by 7 publications

(2 citation statements)

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“…Some authors reported that erythrocytes be come more shperical as they age basing on observations in optical microscope [45,51] while others found no shape alterations [56, 163,194], Results of micropipette measure ments indicate that sphericity defined as 4.84 (volume)2/3/area increases by less than 2% for the densest, as compared with light est. erythrocytes in both human and rabbit; it would mean that red cells do not become spherical as they approach senescence [204], However, it has been argued that since the cells become smaller, the lack of changes in the surface/volume ratio is indicative of an increasingly shperical shape [205].…”

Section: Cellular Shape and Deformabilitymentioning

confidence: 99%

Erythrocyte Aging: Physical and Chemical Membrane Changes

Bartosz¹

1991

Gerontology

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The mammalian erythrocyte is an interesting model for studies of membrane aging. Experimental approaches to this problem involve, first of all, comparison of properties of erythrocytes separated by density because red blood cell age correlates with density in principle. Other approaches to study red cell membrane aging, such as hypertransfusion, are also discussed. A number of physical and chemical changes occur in erythrocytes with aging. Crucial to the elucidation of aging mechanisms is to determine which are primary and which are secondary. Immunoglobulin G binding triggers cellular removal and seems necessary for the recognition of senescent erythrocytes. Cellular deformability decreases while fragility to hemolytic factors generally increases and surface charge density does not alter. Perhaps more important are reactions of reactive oxygen species with membrane constituents and subsequent proteolysis. These are implicated as causative factors in red cell aging, immunoglobulin binding and recognition of senescent erythrocytes.

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Section: Cellular Shape and Deformabilitymentioning

confidence: 99%

Erythrocyte Aging: Physical and Chemical Membrane Changes

Bartosz¹

1991

Gerontology

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“…Studies have been conducted over the years addressing the peculiar characteristics of RBC subpopulations, from younger to older fractions. RBC aging has been reported to correlate with decreased cell volume, size and mean corpuscular volume [14,16], increased mean corpuscular haemoglobin concentration [14] and cell deformability [15].…”

Section: Introductionmentioning

confidence: 99%

A New Method of Assessing Sheep Red Blood Cell Types from Their Morphology

Žaja

Vince

Milas

et al. 2019

Animals

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Simple Summary: Haematological tests are an important diagnostic tool for animal diseases. However, little is known beyond the standardly used haematological methods in sheep, especially those in which the detection of sheep red blood cells (RBCs) shape changes is crucial. Our goal is to obtain sheep RBC morphometric parameters as well as RBC subpopulations based on their morphometric parameters. Morphometric parameters of RBC size and shape were determined from stained blood smears using SFORM, a computer-assisted program for automated cell morphometric measurements. Based on their morphometric parameters, three RBC subpopulations were obtained using principal component and cluster analysis: the smallest and most elongated RBCs, the biggest and most rounded ones, and RBCs of average size and shape. When the values of RBC haematological parameters were higher or above the physiological range, a significantly higher proportion of both average size and shape RBCs, as well as the biggest and most rounded RBCs, was obtained. Since they were obtained from healthy animals, these results indicated the importance of determining morphometric parameters of RBCs and the proportion of each RBC subpopulation, which could serve as a basis for future possibilities in the diagnostic interpretation of haematological disorders in sheep, especially those for which the detection of shape changes in ovine RBCs is crucial.Abstract: Data concerning the morphometric parameters of sheep red blood cells (RBCs) obtained using computer-assisted image analysis have not yet been investigated, and there are no data on any analyses of ovine RBC subpopulations based on their morphometric parameters. The aims of this study are to determine the values of RBC haematological and morphometric size and shape parameters, to form groups according to the obtained values of haematological parameters; to determine the differences in RBC morphometric parameters between the formed groups, and to determine RBC subpopulations and their respective proportions in the formed groups. Thirty-six blood samples were collected from the jugular vein of clinically healthy Lika pramenka sheep, aged between 2 and 5 years. Haematological parameters including haemoglobin (HGB), haematocrit (HTC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular Animals 2019, 9, 1130 2 of 15 haemoglobin concentration (MCHC), and RBC distribution width were analysed using a haematology analyser. Haematological parameters were categorized into two groups: those with lower values or values below the physiological range (Groups 1) and groups with higher values or values above the physiological range (Groups 2). Morphometric parameters of RBCs were determined from stained blood smears using SFORM, a computer-assisted program. Significantly higher values of RBC area, outline, convex, minimal and maximal radius, as well as length and breadth were established in Groups 2 compared to Groups 1 of HGB, HCT, MCV, MCH, and MCHC, respectively. Based on the morphometric p...

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Membrane Lipid Asymmetry in Aging and Apoptosis

Balasubramanian

Schroit

2011

Transmembrane Dynamics of Lipids

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Shape of ageing erythrocytes

Cited by 7 publications

References 0 publications

Erythrocyte Aging: Physical and Chemical Membrane Changes

Erythrocyte Aging: Physical and Chemical Membrane Changes

A New Method of Assessing Sheep Red Blood Cell Types from Their Morphology

Membrane Lipid Asymmetry in Aging and Apoptosis

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