Shab K<sup>+</sup>channel slow inactivation

Carrillo, Elisa; Arias‐Olguín, Imilla I.; Islas, León D; Gómez-Lagunas, Froylán

doi:10.4161/chan.23569

Cited by 10 publications

(11 citation statements)

References 52 publications

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“…Hence, as observed in the Shaker C-type inactivation, 5,6 and in Kv2. 1,12 but in contrast to the fast C-type inactivation of HERG channels, where the channels open from the inactivated state, [13][14][15] recovery from Shab inactivation bypasses the open state.…”

Section: Resultsmentioning

confidence: 99%

“…As it is visually apparent, compared with Na o , both K o + and TEA o significantly slow down (p < 0.05) recovery. In summary, K o + and TEA o speed the entry to 1 and slow the exit from inactivation.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, considering that in Shab both OI and CI are facilitated by K o + and TEA o , we hypothesized that the same inactivated state is reached from either pathway. 1 Hence, in order to support this hypothesis and to further characterize Shab inactivation, we present additional data on the recovery from inactivation. …”

Section: Introductionmentioning

confidence: 97%

“…Curves were compared with the extra-sum-of-squares F-test, as reported. 1 Significance level was set to 0.05.…”

Section: Methodsmentioning

confidence: 99%

“…1 Shab inactivates from both open (OI) and closed states (CI) with a mechanism that is facilitated by external K + (K o + ) and TEA (TEA o ), 1 suggesting that its inactivation qualitatively differs from the C-type inactivation of Shaker, in which these ions exert a characteristic inhibitory effect [2][3][4][5][6] (for a recent commentary, see ref. 7).…”

Section: Introductionmentioning

confidence: 99%

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Recovery from slow inactivation of Shab K⁺channels

Arias‐Olguín

Carrillo²,

Islas³

et al. 2013

Channels

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

“…Curves were compared with the extra-sum-of-squares F-test, as reported. 1 Significance level was set to 0.05.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Recovery from slow inactivation of Shab K⁺channels

Arias‐Olguín

Carrillo²,

Islas³

et al. 2013

Channels

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Gating Modulation of the Tumor‐Related Kv10.1 Channel by Mibefradil

Gómez-Lagunas

Carrillo

Pardo

et al. 2017

Journal Cellular Physiology

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Several reports credit mibefradil with tumor suppressing properties arising from its known inhibition of Ca currents. Given that mibefradil (Mb) is also known to inhibit K channels, we decided to study the interaction between this organic compound and the tumor-related Kv10.1 channel. Here we report that Mb modulates the gating of Kv10.1. Mb induces an apparent inactivation from both open and early closed states where the channels dwell at hyperpolarized potentials. Additionally, Mb accelerates the kinetics of current activation, in a manner that depends on initial conditions. Our observations suggest that Mb binds to the voltage sensor domain of Kv10.1 channels, thereby modifying the gating of the channels in a way that in some, but not all, aspects opposes to the gating effects exerted by divalent cations. J. Cell. Physiol. 232: 2019-2032, 2017. © 2016 Wiley Periodicals, Inc.

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Identification and pharmacological characterization of the voltage‐gated potassium channel Shab in diamondback moth, Plutella xylostella

Huo

Zhu

et al. 2022

Pest Management Science

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Background: Voltage-gated potassium channel Kv2 is the primarily delayed rectifier in insect nerves and muscles involved in several crucial biological processes, including action potential regulation, photoreceptor performance, and larval locomotor. It is a potential molecular target for developing a novel pesticide for mosquitos. However, there are few studies on the Kv2 channel in agricultural pests.Results: The only ⊍-subunit gene of the Kv2 channel in Plutella xylostella (L.), PxShab, was cloned, and its expression profile was analyzed. The relative expression level of PxShab was highest in the pupal stage of both sexes and male adults but lowest in female adults. Meanwhile, PxShab had the highest expression in the head in both larvae and adults. Then, PxShab was stably expressed in the HEK-293 T cell line. Whole cell patch clamp recordings showed an outward current whose current-voltage relationship conformed to a typical delayed-rectifier potassium channel. 20 ∼M quinidine could effectively inhibit the potassium current, while the channel was insensitive to 4-AP even at 10 mM. Several potential compounds and botanical pesticides were assessed, and carvedilol (IC 50 = 0.53 ∼M) and veratrine (IC 50 = 2.22 ∼M) had a good inhibitory effect on the channel. Conclusion:This study revealed the pharmacological properties of PxShab and screened out several high potency inhibitors, which laid the foundation for further functional research of PxShab and provides new insight into designing novel insecticides.

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Shab K⁺channel slow inactivation

Cited by 10 publications

References 52 publications

Recovery from slow inactivation of Shab K⁺channels

Recovery from slow inactivation of Shab K⁺channels

Gating Modulation of the Tumor‐Related Kv10.1 Channel by Mibefradil

Identification and pharmacological characterization of the voltage‐gated potassium channel Shab in diamondback moth, Plutella xylostella

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Shab K+channel slow inactivation

Cited by 10 publications

References 52 publications

Recovery from slow inactivation of Shab K+channels

Recovery from slow inactivation of Shab K+channels

Gating Modulation of the Tumor‐Related Kv10.1 Channel by Mibefradil

Identification and pharmacological characterization of the voltage‐gated potassium channel Shab in diamondback moth, Plutella xylostella

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Resources

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Shab K⁺channel slow inactivation

Recovery from slow inactivation of Shab K⁺channels

Recovery from slow inactivation of Shab K⁺channels