SH3BGRL3 Protein as a Potential Prognostic Biomarker for Urothelial Carcinoma: A Novel Binding Partner of Epidermal Growth Factor Receptor

Chiang, Chengyao; Pan, Chin Chen; Chang, Hong; Lai, Ming Derg; Tzai, Tzong-Shin; Tsai, Yuh Shyan; Ling, Pin; Liu, Hsiao Sheng; Lee, Bi Fang; Cheng, Hong; Ho, Chung Liang; Chen, Shu Hui; Chow, Nan Haw

doi:10.1158/1078-0432.ccr-14-3308

Cited by 35 publications

(41 citation statements)

References 43 publications

(48 reference statements)

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“…SH3BGRL3 was upregulated in glioblastoma multiformand primary bladder cancers as compared to non-tumor tissues, and was detected inurothelial carcinoma patient urine [43, 44]. SH3BGRL3 appears to promote cancer cell proliferation, epithelial-mesenchymal transition (EMT), and cell migration.…”

Section: Discussionmentioning

confidence: 99%

“…SH3BGRL3 appears to promote cancer cell proliferation, epithelial-mesenchymal transition (EMT), and cell migration. We found that SH3BGRL3 interacts with epidermal growth factor receptor (EGFR) at Y1068, Y1086, and Y1173 through Grb2 via its proline-rich motif,and activates the Akt-associated signaling pathway [44]. This protein had not been previously associated with lung cancer.…”

Section: Discussionmentioning

confidence: 99%

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Identification of urine biomarkers associated with lung adenocarcinoma

Wang

Zhang

2017

Oncotarget

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Lung adenocarcinoma (LAC) progression is accompanied by changes in protein levels that may be reflected in body fluids, such as urine. Urine collected from LAC patients (n=34) and healthy controls (n=36) was analyzed via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) combined with weak cationic exchange magnetic beads. The results revealed 76 urinary polypeptides significantly different between LAC patients and normal controls (P<0.05). Twenty-two of these peptides were up-regulated and 54 were down-regulated. Thirteen peptides had average peak intensities >600. Twelve of these 13 peptides were successfully identified using nano-liquid chromatography-tandem MS. Receiver operating characteristic analyses identified seven peptides with superior LAC diagnostic performances. Immunohistochemical staining in 20 paired LAC and adjacent normal tissues showed that IGKC, AAT, SH3BGRL3, osteopontin and gelsolin levels were higher in LAC tissues than in adjacent tissuesand were closely associated with LAC. Urinary peptides assessments may thus provide a novel, noninvasive, repeatable method for detecting and monitoring LAC. New, low-cost detection methods and bioinformatics tools are therefore urgently needed for the analysis of low abundance proteins and peptides in body fluids.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification of urine biomarkers associated with lung adenocarcinoma

Wang

Zhang

2017

Oncotarget

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“…Transcription elongation factor A protein-like 3 1.58 0.030152857 Xq22.2 May be involved in transcriptional regulation (Chiang et al, 2015).…”

Section: Tceal3mentioning

confidence: 99%

Proteomics analysis indicated the protein expression pattern related to the development of fetal conotruncal defects

Zhang

Wang

et al. 2019

Journal Cellular Physiology

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Abnormal development of embryonic conus arteriosus could lead to conotruncal defects in fetal heart, and increase the incidence of fetal congenital heart disease. Tetralogy of Fallot (TOF) is one of the most common forms of congenital heart disease. It may be helpful for us to solve this clinical problem through exploring the molecular mechanisms of development in embryonic congenital heart disease. Proteomics has attracted much attention in understanding the development of human diseases during the past decades. However, there is still little information about the relationship between protein expression pattern and TOF. In this study, we aimed to explore the potential linkage of proteomics and TOF development. Briefly, 121 differentially expressed proteins were identified from a TOF group, compared with a control group. The expression levels of 34 of these proteins were significantly different (>1.5 absolute fold change, p < 0.05) between the two groups. Gene ontology (GO) and pathway analysis showed that these proteins were mainly associated with carbon metabolism, biosynthesis of antibodies, positive regulation of transcription from RNA polymerase II promoter, nucleus, ATP binding, and so on. The ingenuity pathway analysis (IPA) results indicated that 435 of upstream regulators were identified of these differentially expressed proteins, which might be involved in the development of TOF. Data of string analysis showed the protein–protein interaction network among the differentially expressed proteins and regulators, which are related to TOF. In conclusion, our study explored the protein expression pattern of TOF, which might provide new insights into understanding the mechanism of TOF development and afford potential targets for TOF diagnosis and therapy.

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“…Chiang et al performed LC-MS/MS to characterize the urinary proteome of patients with urothelial carcinoma. SH3BGRL3 was found to be positively associated with higher histologic grading and muscle invasiveness [78]. A large number of urinary peptide markers identified for assessment of aggressive disease appear to be fragments of abundant proteins.…”

Section: Clinical Application In Cancermentioning

confidence: 99%

Proteomics and peptidomics: moving toward precision medicine in urological malignancies

2016

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Urological malignancies are a major cause of morbidity and mortality worldwide. Advances in early detection, diagnosis, prognosis and prediction of treatment response can significantly improve patient care. Proteomic and peptidomic profiling studies are at the centre of kidney, prostate and bladder cancer biomarker discovery and have shown great promise for improved clinical assessment. Mass spectrometry (MS) is the most widely employed method for proteomic and peptidomic analyses. A number of MS platforms have been developed to facilitate accurate identification of clinically relevant markers in various complex biological samples including tissue, urine and blood. Furthermore, protein profiling studies have been instrumental in the successful introduction of several diagnostic multimarker tests into the clinic. In this review, we will provide a brief overview of high-throughput technologies for protein and peptide based biomarker discovery. We will also examine the current state of kidney, prostate and bladder cancer biomarker research as well as review the journey toward successful clinical implementation.

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SH3BGRL3 Protein as a Potential Prognostic Biomarker for Urothelial Carcinoma: A Novel Binding Partner of Epidermal Growth Factor Receptor

Cited by 35 publications

References 43 publications

Identification of urine biomarkers associated with lung adenocarcinoma

Identification of urine biomarkers associated with lung adenocarcinoma

Proteomics analysis indicated the protein expression pattern related to the development of fetal conotruncal defects

Proteomics and peptidomics: moving toward precision medicine in urological malignancies

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