SFTA1P, LINC00968, GATA6‑AS1, TBX5‑AS1, and FEZF1‑AS1 are crucial long non‑coding RNAs associated with the prognosis of lung squamous cell carcinoma

Xiong, You-Wen; Zhang, Xinyi; Lin, Zongkai; Xiong, Aizhen; Xie, Shanshan; Liang, Jia; Zhang, Weifang

doi:10.3892/ol.2019.10744

Cited by 20 publications

(25 citation statements)

References 35 publications

(36 reference statements)

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“…Long non-coding RNA (lncRNA) is a type of noncoding RNA with over 200 nucleotides in length and has no protein coding ability and [13,14]. As for the GATA6-AS1 mentioned in this study, it has been con rmed that it is close associated with lung squamous cell carcinoma [15,16]. However, its function and mechanism in LUAD are still not studies until now.…”

Section: Discussionmentioning

confidence: 78%

Long Non-Coding RNA GATA6-AS1 Inhibits Proliferation and Promotes Apoptosis of Lung Adenocarcinoma Through Sponging miR-331-3p and Regulating SOCS1/JAK2/STAT3 Pathway

Huo

Wang

Jiang

et al. 2020

Preprint

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Abstract Background: Accumulating evidence has indicated the remarkable roles of long non-coding RNAs (lncRNAs) as oncogenes or tumor suppressors in many malignancies. The involvement of lncRNA GATA6-AS1 in cancers remains largely undiscovered. Herein, our research was aimed at elucidating the function and mechanism of GATA6-AS1 in lung adenocarcinoma (LUAD).Methods: Gene expression was measured through qRT-PCR and WB. Cell proliferation ratio was determined using CCK-8 and EdU assays. Cell apoptosis ratio was determined using TUNEL and flow cytometry assays. Molecular interactions were examined through RIP, RNA pull-down and luciferase reporter assays.Results: GATA6-AS1 expression was markedly down-regulated in LUAD cell lines. GATA6-AS1 could inhibit LUAD cell proliferation and promote cell apoptosis. Mechanistically, GATA6-AS1 was identified as the molecular sponge for miR-331-3p, whose knockdown in LUAD cells could reinforce the tumor-suppressing effects of GATA6-AS1 overexpression. Moreover, GATA6-AS1 functions as a competing endogenous RNA (ceRNA) through sequestering miR-331-3p to deregulate SOCS1, thus inhibiting JAK2/STAT3 signaling pathway and suppressing LUAD cell viability.Conclusions: These results demonstrate the tumor-suppressing function and mechanism of lncRNA GATA6-AS1 in LUAD cells. The axis of GATA6-AS1/miR-331-3p/SOCS1/JAK2/STAT3 can be adopted as a novel approach for LUAD treatment.

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Section: Discussionmentioning

confidence: 78%

Long Non-Coding RNA GATA6-AS1 Inhibits Proliferation and Promotes Apoptosis of Lung Adenocarcinoma Through Sponging miR-331-3p and Regulating SOCS1/JAK2/STAT3 Pathway

Huo

Wang

Jiang

et al. 2020

Preprint

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“…Extensive studies reported that most of the lncRNAs are abnormally expressed in tumor tissues, and each lncRNA exerts its function through diverse mechanisms in different cancers. According to the previous studies, LINC00968 was upregulated in osteosarcoma and lung squamous cell carcinoma [13,30].…”

Section: Discussionmentioning

confidence: 86%

“…The top 48 hub genes (node degree>=30) were obtained based on degree methodSupplementary FilesThis is a list of supplementary les associated with this preprint. Click to download.…”

mentioning

confidence: 99%

Potential role of long non-coding RNA LINC00968 in luminal A and B breast cancers

Arabpour

Layeghi

Majidzadeh‐A

et al. 2020

Preprint

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Purpose Breast Cancer (BC) is the most frequent malignancy among women worldwide. ER+ breast cancers (luminal A and B subtypes) comprise up to 70% of all BC patients. Long non-coding RNAs (lncRNAs) are regulatory non-coding transcripts and longer than 200 nucleotides. LncRNAs can affect many biological and pathological processes and dysregulation of them is related to many human cancers. The potential role of LINC00968 lncRNA in luminal A and B breast cancer pathogenesis is still unclear. Methods Seventy-one pairs of tumor and adjacent non-tumor tissue specimens of luminal A and B breast cancer were used to analyze the expression of LINC00968. Furthermore, two luminal A cell lines, MCF7 and T47D, were used to evaluate the expression of LINC00968 compared with a non-malignant breast cell line, MCF10A. Moreover, we have done multiple bioinformatic analyses for a better understanding of the potential roles of LINC00968 in luminal BC. Results Our data revealed the significant downregulation of LINC00968 in luminal tumor tissues and cell lines. LINC00968 expression was negatively associated with tumor stage and lymph node metastasis. Bioinformatic analyses indicated that LINC00968 might be involved in blood vessel development, angiogenesis, and might be participated in interaction of ECM constituents with cancer cells. LINC00968 might have functions in some cancer-related signaling pathways, like PI3K/Akt, ECM-receptor interaction signaling pathway, and PPAR signaling pathway. Conclusions Downregulation of LINC00968 might promote carcinogenesis of luminal BC. LINC00968 might act as a tumor suppressor gene and might also promote invasion and metastasis of luminal BC.

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“…17 RNA sequencing data indicated that GATA6-AS1 was one of the most significantly downregulated lncRNAs in LUSC. 18 We firstly used online tool to analyze the expression of GATA6-AS1 in TCGA data of various cancer types and GTEx project using GEPIA software. Interestingly, GATA6-AS1 was downregulated in multiple cancer types (11 out of 33) including LUAD and LUSC.…”

Section: Discussionmentioning

confidence: 99%

“…15,16 LncRNA GATA6-AS1 is a recently discovered cancer-related lncRNA. 17,18 However, its function in lung cancer remains unknown.…”

Section: Introductionmentioning

confidence: 99%

<p>Long Non-Coding RNA GATA6-AS1 Sponges miR-324-5p to Inhibit Lung Cancer Cell Proliferation and Invasion</p>

Wang

Pan

Yang

et al. 2020

OTT

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Background: Long non-coding RNAs (lncRNAs), which are key regulators of gene expression, are involved in lung cancer progression. Although numerous differentially expressed lncRNAs have been reported, merely a limited number of studies have been performed to verify their functions in lung cancer. Methods: RNA sequencing data were re-analyzed to investigate the GATA6-AS1 expression in lung cancer. RT-qPCR was performed to verify the expression of GATA6-AS1 in collected tissue samples and cell lines. CCK-8 and transwell assays were carried out to evaluate the role of GATA6-AS1 in lung cancer cells. Dual-luciferase reporter assay and bioinformatic analysis were used to explore the miRNA which can be sponged by GATA6-AS1 in lung cancer cells. Results: Currently, we focused on exploring the role and mechanisms of GATA6-AS1 in lung cancer. Expression of GATA6-AS1 was decreased in lung cancer based on the analysis of RNA sequencing dataset, TCGA data and RT-qPCR of clinical tissue samples. Via overexpression of GATA6-AS1, it was revealed that GATA6-AS1 inhibited lung cancer cell proliferation and invasion. Oncogene miR-324-5p was predicted to interact with GATA6-AS1. RT-qPCR and dual-luciferase reporter assay verified the regulation of miR-324-5p by GATG6-AS1 in lung cancer cells. Overexpression of GATA6-AS1 increased the expression of FBXO11 and SP1, two target genes of miR-324-5p. We further showed that miR-324-5p mimic reversed the effect of GATA6-AS1 overexpression in lung cancer cells. Conclusion: Overall, our findings demonstrated GATA6-AS1 as a novel tumor suppressor in lung cancer.

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SFTA1P, LINC00968, GATA6‑AS1, TBX5‑AS1, and FEZF1‑AS1 are crucial long non‑coding RNAs associated with the prognosis of lung squamous cell carcinoma

Cited by 20 publications

References 35 publications

Long Non-Coding RNA GATA6-AS1 Inhibits Proliferation and Promotes Apoptosis of Lung Adenocarcinoma Through Sponging miR-331-3p and Regulating SOCS1/JAK2/STAT3 Pathway

Long Non-Coding RNA GATA6-AS1 Inhibits Proliferation and Promotes Apoptosis of Lung Adenocarcinoma Through Sponging miR-331-3p and Regulating SOCS1/JAK2/STAT3 Pathway

Potential role of long non-coding RNA LINC00968 in luminal A and B breast cancers

<p>Long Non-Coding RNA GATA6-AS1 Sponges miR-324-5p to Inhibit Lung Cancer Cell Proliferation and Invasion</p>

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