<p>Long Non-Coding RNA GATA6-AS1 Sponges miR-324-5p to Inhibit Lung Cancer Cell Proliferation and Invasion</p>

Abstract

Background: Long non-coding RNAs (lncRNAs), which are key regulators of gene expression, are involved in lung cancer progression. Although numerous differentially expressed lncRNAs have been reported, merely a limited number of studies have been performed to verify their functions in lung cancer. Methods: RNA sequencing data were re-analyzed to investigate the GATA6-AS1 expression in lung cancer. RT-qPCR was performed to verify the expression of GATA6-AS1 in collected tissue samples and cell lines. CCK-8 and transwell assays were carried out to evaluate the role of GATA6-AS1 in lung cancer cells. Dual-luciferase reporter assay and bioinformatic analysis were used to explore the miRNA which can be sponged by GATA6-AS1 in lung cancer cells. Results: Currently, we focused on exploring the role and mechanisms of GATA6-AS1 in lung cancer. Expression of GATA6-AS1 was decreased in lung cancer based on the analysis of RNA sequencing dataset, TCGA data and RT-qPCR of clinical tissue samples. Via overexpression of GATA6-AS1, it was revealed that GATA6-AS1 inhibited lung cancer cell proliferation and invasion. Oncogene miR-324-5p was predicted to interact with GATA6-AS1. RT-qPCR and dual-luciferase reporter assay verified the regulation of miR-324-5p by GATG6-AS1 in lung cancer cells. Overexpression of GATA6-AS1 increased the expression of FBXO11 and SP1, two target genes of miR-324-5p. We further showed that miR-324-5p mimic reversed the effect of GATA6-AS1 overexpression in lung cancer cells. Conclusion: Overall, our findings demonstrated GATA6-AS1 as a novel tumor suppressor in lung cancer.

RETRACTED ARTICLE: Long non-coding RNA GATA6-AS1 upregulates GATA6 to regulate the biological behaviors of lung adenocarcinoma cells

“…Overall, these findings exhibited that GATA6-AS1 exerted tumor suppressive function in the cellular processes of LUAD. Previously, GATA6-AS1 was indicated to suppress proliferation and migration of LUAD cells by binding with miR-543 to upregulate RKIP [ 36 ] and by sponging miR-324-5p to increase the expression of FBXO11 and SP1 [ 37 ]. GATA6-AS1 is associated with the favorable prognosis of lung squamous cell carcinoma [ 38 ].…”

Computational Ensemble Gene Co-Expression Networks for the Analysis of Cancer Biomarkers

“…The GATA6-AS1 lncRNA has been linked to the expression of F-box proteins [85], which play an important role in the degradation of key proteins in cellular regulation and tumorigenesis [86]. It has been studied in the context of lung, gastric, and ovarian cancer in association with the inhibition of signaling pathways and prevention of epithelialmesenchymal transition [87,88]. The hub gene GATA6-AS1 was in the green cluster and had 26 interactions.…”

The functions of long noncoding RNAs on regulation of F-box proteins in tumorigenesis and progression

“…LincRNA GATA6-AS1 promoted GATA6 expression and controlled the behaviors of lung cancer cells ( 146 ). In lung cancer cells, lincRNA GATA6-AS1 suppressed cell proliferation and invasive ability ( 147 ). Using several approaches, including RNA sequencing dataset, RT-qPCR and TCGA data, one group found that GATA6-AS1 expression levels were downregulated in lung cancer tissues ( 147 ).…”

“…In lung cancer cells, lincRNA GATA6-AS1 suppressed cell proliferation and invasive ability ( 147 ). Using several approaches, including RNA sequencing dataset, RT-qPCR and TCGA data, one group found that GATA6-AS1 expression levels were downregulated in lung cancer tissues ( 147 ). Moreover, GATA6-AS1 overexpression increased the expression of FBXO11 and SP1 via sponging miR-324-5p, contributing to enhancement of invasion and proliferation in lung cancer cells.…”

“…Moreover, GATA6-AS1 overexpression increased the expression of FBXO11 and SP1 via sponging miR-324-5p, contributing to enhancement of invasion and proliferation in lung cancer cells. Furthermore, miR-324-5p overexpression abolished the effects of GATA6-AS1 upregulation in lung cancer ( 147 ). In a word, lincRNA GATA6-AS1 might regulate miR-324-5p/FBXO11 axis and facilitated lung cancer development.…”