Sexually dimorphic expression of sexual differentiation genes in the internal genital organs of Japanese quail embryos

Tsukahara, Shinji; Morishita, Masahiro; Sasaki, Shiho; Wakayama, Kanta; Kobayashi, Kazuyo; Ohno, Koichi; Kawashima, Takaharu

doi:10.1016/j.ygcen.2021.113917

Cited by 8 publications

(8 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RT‐qPCR analysis was performed as reported previously (Tsukahara et al, 2021). In brief, total RNA was extracted by using an RNeasy Mini Kit (Qiagen GmbH, Hilden, Germany) or an RNeasy Micro Kit (Qiagen), and cDNA was synthesized by using a TaKaRa Prime Script RT Reagent kit (TaKaRa Bio, Inc., Otsu, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…Plasma concentrations of estradiol and testosterone in embryos were measured using a previously described procedure with slight modifications (Tsukahara et al, 2021). Briefly, the lipophilic fraction of plasma samples was extracted with diethyl ether.…”

Section: Methodsmentioning

confidence: 99%

“…The genes encoding the steroidogenic enzymes needed for estrogen synthesis—cholesterol side‐chain cleavage enzyme (P450 scc ), 17α‐hydroxylase/C17–20 lyase (P450 17α, lyase ), 3β‐hydroxysteroid dehydrogenase (3β‐HSD), 17β‐hydroxysteroid dehydrogenase (17β‐HSD), and aromatase (P450 arom )—are highly expressed in the left ovary of female Japanese quail embryos but moderately or scarcely expressed in the testes of male quail embryos (Mattsson, Olsson, & Brunström, 2008; Tsukahara et al, 2021). This suggests that sex differences in the expressions of these genes could be responsible for the female‐biased sex difference in estrogen synthesis.…”

Section: Introductionmentioning

confidence: 99%

“…Androgen‐mediated activation of androgen receptor (AR) is important for the morphogenesis of the left ovary in the embryonic period (Katoh et al, 2006). In the late embryonic period, the left ovary and left MD express these receptors in greater numbers than do the testes and right MD, respectively (Tsukahara et al, 2021), suggesting that such lopsided receptor expression shapes the organization of the female reproductive organs. Anti‐Müllerian hormone (AMH), which is synthesized by the gonads and affects the gonads and MDs by binding to AMH receptor type 2 (AMHR2) during embryo development (Tsukahara et al, 2021), is involved in gonadal growth as well as in the regression of the MDs (Lambeth et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…In the late embryonic period, the left ovary and left MD express these receptors in greater numbers than do the testes and right MD, respectively (Tsukahara et al, 2021), suggesting that such lopsided receptor expression shapes the organization of the female reproductive organs. Anti‐Müllerian hormone (AMH), which is synthesized by the gonads and affects the gonads and MDs by binding to AMH receptor type 2 (AMHR2) during embryo development (Tsukahara et al, 2021), is involved in gonadal growth as well as in the regression of the MDs (Lambeth et al, 2015). Thus, sexual differentiation and development of the reproductive organs in birds require expression of the genes involved in steroidogenesis as well as the action of sex steroids and AMH.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

In ovo o,p′‐DDT exposure induces malformation of reproductive organs and alters the expression of genes controlling sexual differentiation in Japanese quail embryo

Win‐Shwe,

Abe,

Sakiyama

et al. 2023

J of Applied Toxicology

Self Cite

View full text Add to dashboard Cite

In ovo exposure to o,p′‐dichloro‐diphenyl‐trichloroethane (o,p′‐DDT) impairs reproduction by inducing malformation of the reproductive organs in birds, although the mechanism remains unclear. Here, we examined the effects of o,p′‐DDT on the development of the reproductive organs, the expression of genes controlling sexual differentiation, and the plasma concentrations of testosterone and estradiol in Japanese quail embryos. o,p′‐DDT‐containing sesame oil was injected into the yolk sac on Embryonic Day (E) 3 at a dose of 500, 2,000, or 8,000 μg per egg. On E15, the reproductive organs were observed; the gonads and Müllerian ducts (MDs) were sampled to measure the mRNA of steroidogenic enzymes, sex steroid receptors, anti‐Müllerian hormone (AMH), and AMH receptor 2 (AMHR2); blood samples were collected to assay plasma testosterone and estradiol levels; and the gonads were used for histological analysis. o,p′‐DDT dose‐dependently increased the prevalence of hypertrophic MDs in females and residual MDs in males. In female MDs, o,p′‐DDT dose‐dependently decreased estrogen receptor (ER) α, ERβ, and AMHR2 mRNA expression. o,p′‐DDT dose‐dependently induced left‐biased asymmetry of testis size, and ovary‐like tissue was found in the left testis after exposure to 8,000 μg per egg o,p′‐DDT, although asymmetric gene expression did not occur. o,p′‐DDT did not affect ovarian tissue but did decrease 17α‐hydroxylase/C17‐20 lyase mRNA expression and dose‐dependently increased ERβ mRNA expression. o,p′‐DDT decreased plasma testosterone concentrations in females. These findings suggest that o,p′‐DDT induces hypertrophy of the MDs and ovarian tissue formation in the left testis. Abnormal MD development may be linked to altered gene expression for sensing estrogens and AMH signals.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%