Objective: to determine the prevalence of sexual dysfunctions in pregnant women of the Coffee Axis, and to evaluate their impact on sexuality during each quarter. Material and methods: prospective observational study, descriptive in nature; carried out in three private level III clinics in the Coffee Region, Colombia. Women over 18 years of age, with a confirmed diagnosis of pregnancy in the first trimester, without sexually transmitted infections, pregnancy with a single fetus, stable partner and without contraindications for sexual activity were included. The sampling was consecutive for convenience. The Index of Female Sexual Function (IFSF) was applied as an instrument. Socio-demographic variables, sexual and reproductive health history, and by type of sexual dysfunction evaluated were measured. The data were processed with the statistical analysis program SPSS, version 19. Results: 1440 pregnant women were analyzed. The mean age was 24.9 ± 6.57 years. The mean IFSF score in the total population was 29.47 ± 5.48 points (minimum 9.71 and maximum 30.58). The prevalence of sexual dysfunctions in the studied group, in the first trimester, was 39.79%, in the second 44.37% and 71.87% in the third. The sexual dysfunction most frequently present in the total population was desire disorder (71.94%), followed by alteration of orgasm (39.79%) and, in third place, the presence of pain (26, Four. Five%). Sexual satisfaction decreased 20.62%. Conclusions: a revealing impact of pregnancy on sexuality is observed in women from the Coffee Region, which can have negative effects on general well-being and on the couple relationship. There is a prevalence of sexual dysfunctions close to ¾ parts of the population studied, characterized mainly by desire and orgasm disorders.