Sexual function in women of fertile age with epilepsy

Santos, Ana Maria Cruz; Filho, Humberto Castro Lima; Siquara, Gustavo Marcelino; Lopes, Josiane Mota; Bastos, Claudilson; Brito, Milena Bastos

doi:10.1016/j.yebeh.2021.108399

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…The impact of anti-epileptic drugs on the concentrations of reproductive hormones could indicate an association between the occurrence of reproductive disorders and the consumption of these medications. These correlations in epileptic patients are primarily based on several studies showing that alterations in SHBG are linked with sexual dysfunction 25 . Evidence shows that VPA effectively contributes to the metabolic pathways of estrogens and androgens.…”

Section: Discussionmentioning

confidence: 99%

Assessment of circulating miRNA-218, miRNA-222, and miRNA-146 as biomarkers of polycystic ovary syndrome in epileptic patients receiving valproic acid

Rajabi,

Kalantar,

Mojodi

et al. 2023

Biomed. Res. Ther.

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Introduction:This study was designed to evaluate the relationship between taking sodium valproate (VPA) and the onset of polycystic ovarian syndrome (PCOS) in women with epilepsy (WWE) and analyze the biochemical factors and expression levels of three miRNAs as diagnostic or predictive biomarkers. These miRNAs target numerous genes and molecular pathways involved in hyperandrogenism, insulin resistance, and obesity in PCOS patients. Methods: The study was conducted on 120 WWE aged 18-35 years before and after monotherapy with VPA and 120 women with PCOS (WWP). After collecting the plasma samples of patients, the total RNA was extracted. Then, the miRNA-218, miRNA-222, and miRNA-146 expression levels were determined via qRT-PCR. Results: In this study, the relative expression levels of miRNA-146 and miRNA-218 showed a significant increase in the WWP and treated epileptic patients (P ≤ 0.01). However, the expression level of miRNA-222 significantly (P ≤ 0.05) reduced. Our finding showed a significant increase in the concentrations of anti-Müllerian hormone (AMH), free testosterone, and estradiol and an increased LH/FSH ratio after treatment compared with pre-treatment with VPA (P ≤ 0.05). Conclusion: Significant changes were observed in the expression of the examined miRNAs after receiving VPA, especially miRNA-218. In addition, a significant correlation was found between PCOS and AMH, free testosterone, estradiol, and the LH/FSH ratio. Therefore, the miRNA-218 expression and these biochemical factors are valuable biomarkers for predicting PCOS symptoms. They are cost-effective for controlling side effect and timely medication adjustments in patients receiving VPA.

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Section: Discussionmentioning

confidence: 99%

Assessment of circulating miRNA-218, miRNA-222, and miRNA-146 as biomarkers of polycystic ovary syndrome in epileptic patients receiving valproic acid

Rajabi,

Kalantar,

Mojodi

et al. 2023

Biomed. Res. Ther.

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“…However, women's sexual response process is not staged and unidirectional, there is no sequence that sexual desire, sexual arousal and orgasm are often mixed together, so this model cannot re ect the real process of sexual response. At present, most researchers are accustomed to using ovariectomized female rats as the objection of PGAD 22 , this only represents the sexual dysfunction caused by menopause, and cannot represent the characteristics of premenopausal 30,31 . Because of these, we need a comprehensive animal model which can fully re ects the sexual characteristics of PGAD patients.…”

Section: Discussionmentioning

confidence: 99%

Established a comprehensive mice model of persistent genital arousal disorder and investigated the mechanism of VEGF-ERK signal pathing in vaginal microvascular injury

Liu

et al. 2022

Preprint

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Aim: (1) Simulating vaginal delivery and menopause to establish a mouse model of PGAD; (2) screen out a set of detection methods for quantifying and evaluating mouse sexual function; (3) further explore the relationship and possible mechanism of vaginal microvascular injury and PGAD.Methods: Animal study about established a PGAD mouse model with vaginal dilation and ovariectomized, and ligated vaginal microvascular to compared effects of blood flow on sexual behavior with sham. Animals: C57/B6J mice. Intervention: 8-week-old post-childbirth mice were vaginal dilation and ovariectomy and reared for 1 or 2 months. In addition, ligated microvascular around the vagina to build a vaginal ischemia model.Main Outcome Measures: Video cameras continuously recorded the mice's sexual behavior, vaginal temperature and lubricating were observed by thermometer and water absorption test paper, vagina surface tension was observed by multi-channel physiological recorder. Various stains were used to observe the vaginal morphology, blood vessels, nerves, muscles and fibers of vaginal tissue.Results: (1) Different modeling methods and time will cause atrophy in the vagina and uterus in different groups (F=169.4, p<0.01; F=114.3, p<0.01), among which VD2H has less effect, while OVX and VD4H have more. The influence of the two methods combination makes the atrophy more obvious. (2) Vaginal dilation had no significant effect on E2, T and DA (F=147.6, p>0.05), but the contents of these three hormones decreased after ovariectomy (F=480.9, p<0.01). (3) Except for VD2H, the acceptance of sexual response in other groups were decreased (F=58.4, p<0.01), the aggressive was increased (F=121.1, p<0.01), and the lordosis were also decreased (F=67.9, p<0.01). Besides, the vaginal lubrication and vaginal contractions were decreased (F=61.3, p<0.01), but the vaginal temperature did not change significantly in each group (F=38.4, p>0.05). (4) Different modeling methods caused different damage and atrophy in the vaginal tissue, which showed that the thickness of epithelial and muscle layer was reduced, the fibrosis was deepened, the content of muscle, nerve fibers and glycogen were reduced. The above phenomenon was most obvious in the OVX&VD4H group, and the VD2H group was the least. Compared with 1 month, the vaginal tissue had restored at 2 months in most groups. (5) Ovariectomy and vaginal dilation both decreased the number of vaginal microvascular (F=5.4, p<0.05), and the contents of VEGF and MMP-9 decreased (F=367.9, p<0.01; F=121.3, p<0.01), particularly, VD4H decreased more than VD2H (F=117.3, p<0.01), OVX&VD4H was more than OVX&VD2H (F=413.3, p<0.01), and in 2 months, their levels all increased to varying degrees. (6) Vaginal atrophy occurred after blood flow occlusion, manifested as uniform atrophy of the entire vaginal thickness and aggravated fibrosis. The weights of vagina and uterus were both lower than sham group (F=13.7, p<0.01; F= 12.1, p<0.01), the estrous cycle was disturbed, and these manifestations were more obvious in bilateral blood flow occlusion than unilateral occlusion. (7) The number of microvascular in the vaginal tissue were decreased after blood flow occlusion (F=16.4, p<0.01), and the contents of VEGF and MMP-9 also decreased (F=669.1, p<0.01; F=793.1, p<0.01), in which bilateral blockade was more obvious than unilateral blockade. After WB experiment, it was found that the contents of VEGF and ERK decreased after blood flow blockade, and the decrease in bilateral blockade group was more than that in unilateral blockade group.Conclusion: By simulating different modeling methods and time, we believe that a stable and novel PGAD mouse model can be established after ovariectomy combined with vaginal dilation for 2 hours (OVX&VD2H) and rearing for 1 month. In addition, through a large number of experiments, we screened that sexual behavior, vaginal lubricating, vagina surface tension can be used to detect sexual function in mice objectively and accurately, but vaginal temperature not suitable for the detection of mouse sexual function. Finally, we further verified that microvascular injury can significantly appeared in the model, and the VEGF-ERK signaling pathway is a possible mechanism involved in microvascular injury.

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Prevalence and Diagnosis of Sexual Dysfunction in People with Epilepsy

Rathore

Radhakrishnan

2022

Neurologic Clinics

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Sexual function in women of fertile age with epilepsy

Cited by 4 publications

References 17 publications

Assessment of circulating miRNA-218, miRNA-222, and miRNA-146 as biomarkers of polycystic ovary syndrome in epileptic patients receiving valproic acid

Assessment of circulating miRNA-218, miRNA-222, and miRNA-146 as biomarkers of polycystic ovary syndrome in epileptic patients receiving valproic acid

Established a comprehensive mice model of persistent genital arousal disorder and investigated the mechanism of VEGF-ERK signal pathing in vaginal microvascular injury

Prevalence and Diagnosis of Sexual Dysfunction in People with Epilepsy

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