Sex differential marker FD for rapid sex identification of Litsea cubeba

Abstract

ABSTRACT. Litsea cubeba is an important economic tree in China.Sex identification of the species is required to reduce breeding costs. Molecular biology is an ideal method to achieve this aim because of the lack of morphological differences between male and female plants, especially at the seedling stage. Sequence-related amplified polymorphism was used to amplify sex-related bands. Following sequencing, the amplified fragment Dwas used to create a sequencecharacterized amplified region (SCAR) marker, FD. The SCAR marker is approximately 750 bp, is female-specific, and is expected to be useful for L. cubeba breeding programs. Furthermore, the amplified fragment L had homology to sex-determining-related genes of other species. Quantitative real-time polymerase chain reaction analysis of this fragment during flower bud development identified expression differences between male and female plants.

Assessment of genetic diversity, population structure and sex identification in dioecious crop, Trichosanthes dioica employing ISSR, SCoT and SRAP markers

“…Prior to our study, SRAP markers have been used for sex identification only in few plants. Female sex specific SRAP markers have been identified in Buchloe dactyloides [20], Idesia polycarpa [21], Ficus pumila [74] and Litsea cubeba [75].…”

Sexual dimorphism, temporal niche differentiation, and evidence for the Jack Sprat effect in an annual dioecious plant

“…However, recent application of molecular approaches to studies of dioecious species has led to the development of genetic markers enabling unambiguous identification of males and females, even at the seed stage (Stehlik & Barrett, 2005). These markers have most often been developed for breeding programs on wild species of potentially important economic importance or in already domesticated species (e.g., Khadke et al, 2012; Cherif et al, 2013; Sun et al, 2014; Wu et al, 2015; Li & Ye, 2020; Wang et al, 2020). Less often they have been applied in ecological and evolutionary studies of dioecious populations (see Eppley et al, 1998; Korpelainen, 2002; Stehlik & Barrett, 2005; Morgan et al, 2020).…”

The use of soil pollen to determine the sex of overhead individuals of a temperate dioecious shrub

“…Besides using flowering and fruiting, sex chromosomes and genetic markers have been used to determine the sex of individuals for some species (e.g., Joseph et al, 2014; Wang et al, 2015; Wu et al, 2015). One major advantage of these methods is that the sex can be determined for prereproductive individuals, as early as seedlings (e.g., Joseph et al, 2014; Sun et al, 2014).…”