Sex Determination in 58 Bird Species and Evaluation of <scp>CHD</scp> Gene as a Universal Molecular Marker in Bird Sexing

Vučićević, Miloš; Stevanov-Pavlović, Marija; Stevanović, Jevrosima; Bošnjak, None Jasna; Gajić, Bojan; Aleksić, Nevenka; Stanimirović, Zoran

doi:10.1002/zoo.21010

Cited by 63 publications

(69 citation statements)

References 33 publications

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“…The ambiguous results of the primers CHD1Wr/NP/ CHD1Zr compared to HPF/HPR and in the individuals of previously known sex are similar to those reported for the primers P2/P8 and 2550F/2718R [Vucicevic et al, 2012] and could be explained by the variability of length of the intronic region of CHD in some species or even in some individuals [Kahn et al, 1998;Fridolfsson and Ellegren, 1999] or by the absence of heteromorphic sex chromosomes [Wang et al, 2007]. However, possible modifications in the amplification protocols and even in the electrophoresis procedure (use of polyacrylamide gels and restriction enzymes) could improve band resolution with these markers.…”

Section: Discussionsupporting

confidence: 61%

Molecular Sexing of the White-Winged Guan (Penelope albipennis) and Other Wild Birds of the North of Peru

Casana

Ruiz

Sandoval

et al. 2018

Sex Dev

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The use of accurate and reliable techniques for sex determination of wild birds is of special importance in captive breeding programs, especially in birds with monogamous, aggressive behavior, with absence of copulation, and with a low hatching rate. Using PCR, we evaluated the relative efficacy of primers HPF/HPR and CHD1Wr/NP/CHD1Zr in the amplification of the chromo-helicase-DNA binding 1 (CHD1) gene for sex determination in Penelope albipennis and 8 other species of cracids, 4 species of falconids, 4 species of accipitrids, and 3 species of psittacines. Primer effectiveness was compared using previously sexed bird samples. The HPF/HPR primer set was found to demonstrate a better performance and reliability. Therefore, these primers should be used to determine the sex of juvenile birds to avoid or minimize incompatibilities during the selection of potential breeding pairs.

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Section: Discussionsupporting

confidence: 61%

Molecular Sexing of the White-Winged Guan (Penelope albipennis) and Other Wild Birds of the North of Peru

Casana

Ruiz

Sandoval

et al. 2018

Sex Dev

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“…In Platalea and also Accipitridae and Anatidae species previous attempts at molecular sexing gave ambiguous results (Wang et al, 2007;Ong and Vellayan, 2008). Also a larger number of samples was included for species where the protocol by Vucicevic et al (2012) was previously employed, confirming the reliability and robustness of the method.…”

Section: Visualization Of Pcr Productsmentioning

confidence: 97%

Molecular sex determination of 20 bird species protected in the Republic of Serbia

Stevanov-Pavlović¹,

Vučićević²,

Bošnjak³

et al. 2013

ACTA VET-BEOGRAD

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Considering that more than 50% of bird species are monomorphic, the identification of gender based on phenotypic characteristics is extremely difficult. The aim of this study is sex determination in species inhabiting the Republic of Serbia, all under the state protection and declared by IUCN as endangered. DNA was isolated from feathers. Sex determination was based on sex-specific CHD gene amplified by 2550F/2718R primer set. Sexing gave good results in all 91 samples from 20 species including 6 species where molecular sexing has not previously been successful. [Projekat Ministarstva nauke Republike Srbije, br. 46002

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“…; Vucicevic et al . ). This easy and accurate technique based on PCR (but see Robertson & Gemmell ) has been widely applied not only for molecular sexing to fresh tissue samples (e.g.…”

Section: Introductionmentioning

confidence: 97%

Sex determination in the wild: a field application of loop‐mediated isothermal amplification successfully determines sex across three raptor species

Centeno‐Cuadros

Abbasi

Nathan

2016

Molecular Ecology Resources

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18PCR-based methods are the most common technique for sex determination of birds. 19Although these methods are fast, easy and accurate, they still require special facilities 20 that preclude their application outdoors. Consequently, there is a time lag between 21 sampling and obtaining results that impedes researchers to take decisions in situ and in 22 real time considering individuals' sex. We present an outdoor technique for sex 23 determination of birds based on the amplification of the duplicated sex-chromosome-24 specific gene Chromo-Helicase-DNA binding protein using a Loop-Mediated 25Isothermal Amplification (LAMP). We tested our method on Griffon Vulture (Gyps 26 fulvus), Egyptian Vulture (Neophron percnopterus) and Black Kite (Milvus migrans) 27 (family Accipitridae). We introduce the first fieldwork procedure for sex determination 28 of animals in the wild, successfully applied to raptor species of three different 29 subfamilies using the same specific LAMP primers. This molecular technique can be 30 deployed directly in sampling areas since it only needs a voltage inverter to adapt a 31 thermo-block to a car lighter and results can be obtained by the unaided eye based on 32 colour change within the reaction tubes. Primers and reagents are prepared in advance 33 to facilitate their storage at room temperature. We provide detailed guidelines how to 34 implement this procedure, which is simpler (no electrophoresis required), cheaper and 35 faster (results in ca. 90 minutes) than PCR-based laboratory methods. Our successful 36 cross-species application across three different raptor subfamilies posits our set of 37 markers as a promising tool for molecular sexing of other raptor families and our field 38 protocol extensible to all bird species.

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Sex Determination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing

Cited by 63 publications

References 33 publications

Molecular Sexing of the White-Winged Guan (<b><i>Penelope albipennis</i></b>) and Other Wild Birds of the North of Peru

Molecular Sexing of the White-Winged Guan (<b><i>Penelope albipennis</i></b>) and Other Wild Birds of the North of Peru

Molecular sex determination of 20 bird species protected in the Republic of Serbia

Sex determination in the wild: a field application of loop‐mediated isothermal amplification successfully determines sex across three raptor species

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