Molecular sex determination of 20 bird species protected in the Republic of Serbia

Stevanov-Pavlović, Marija; Vučićević, Miloš; Bošnjak, None Jasna; Stevanović, Jevrosima; Dimitrijević, Vojin; Rеsаnоvić, Rаdmilа; Stanimirović, Zoran

doi:10.2298/avb1301045s

Cited by 9 publications

(5 citation statements)

References 19 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When it comes to sex determination in sampled birds using a dye-labelled CHD1F/CHD1R primer set, capillary electrophoresis proved to be an accurate, fast, and reliable method. Our results confi rmed that the specifi ed method enabled accurate sex determination in all tested species, although end point PCR approach was also highly successful, as shown here, but also in previous studies [7,9,10,26,27].…”

Section: Discussionsupporting

confidence: 89%

“…To reduce wildlife traffi cking and illegal hunting, international organizations and national agencies highly depend on forensic investigations which include species and sex identifi cation of animals [7][8][9]. In the last decades, molecular techniques have become very valuable forensic tools that enable, among other things, identifi cation of species from a very low quantity of samples [2,10,11] of various types (e.g.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Potential of Inter- and Intra-Species Variability of CHD1 Gene in Birds as a Forensic Tool

et al. 2021

Self Cite

View full text Add to dashboard Cite

Nowadays, illegal trafficking and smuggling of animals are among the greatest threats to many avian species. Most commonly smuggled birds belong to parrots, song birds, raptor species and owls. All of these species are protected by national and international legislations. In order to prevent and reduce wildlife trafficking, DNA methods have become an important forensic tool in species and sex identification. In this study, CHD1R/CHD1F primer pair was used to amplify a part of the CHD1 gene from 65 birds that belong to 43 species. For 36 species this is the first time that the length of CHD1 amplicons was measured for the purpose of species determination. The results were visualized using capillary electrophoresis and enabled simultaneous determination of sex and species. Based on the number of amplicons (two in females, one in males) sex was successfully determined in all species, even in cases where gel electrophoresis failed to give results. Moreover, the species was successfully determined in most bird species based on the species-specific sizes of CHD1 amplicon. The method used in this study is of great importance for veterinary forensic medicine and the prevention of wildlife smuggling. Still, further work is necessary to confirm the effectiveness of the method in all bird species.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Potential of Inter- and Intra-Species Variability of CHD1 Gene in Birds as a Forensic Tool

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…There is a report of infection of 4.6% stray domestic cats in Dubai with this acanthocephalan [17]. Investigations on protected bird species are of interest for other authors [18].…”

Section: Discussionmentioning

confidence: 97%

First Report on Centrorhynchus Aluconis in Common Buzzard (Buteo Buteo) in Northwest Iran

et al. 2014

View full text Add to dashboard Cite

In July 2012 a male Common Buzzard (Bute obuteo) from the Department of Environment of East Azerbaijan was sent to the Parasitology Laboratory at the School of Specialized Sciences of Veterinary Medicine, Science and Research Unit, Tehran Islamic Azad University. Gastrointestinal parasites were isolated and sent to the Iranian National Parasitology Museum in order to specify the diagnosis. It was determined that the bird was infected with the acanthocephalan Centrorhynchu saluconis. This is the fi rst report of Common Buzzard infestation with this parasite in Iran.

show abstract

“…In that experiment, C-banding was performed using the peripheral blood lymphocyte division phase, and the W chromosome was successfully identified, allowing the sex of the early embryos of intergeneric hybrids to be determined. In the present study, we used C-banding to identify W chromosomes, in combination with the morphological identification of Z chromosomes, which greatly improved the accuracy of sex determination of birds, providing a safe and reliable sex identification test for certain rare avian species [21,22] .…”

Section: G-banding Is a Chromosomal Banding Techniquementioning

confidence: 99%

Tavuk, Bıldırcın ve Hibritlerinin Kromozom Karyotipleri ve Bantlanma Modeli Analizi

Zhu¹,

Xie²,

Heng³

et al. 2018

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

To explore the incompatibility of hybrids between chickens and quails at the chromosome level, in the present study, chickens, quails, and their hybrids were selected and their chromosome karyotype and banding patterns were analyzed. The methods used comprised pre paring chromosomes from air-dried peripheral blood lymphocytes, the embryo method, G-banding, and C-banding techniques. The result revealed that the number of chromosomes (2n) of chicken, quail, and their hybrids was 78, with 10 pairs of macrochromosomes and 29 pairs of microchromosomes; however, there were some remarkable differences in chromosome morphology. There were significant differences in G-banding patterns between chickens, quails, and their hybrids, among which chickens chromosomes were divided into 32 zones with 155 bands, including 71 positive bands. The quails were divided into 28 zones, with 138 bands, including 61 positive bands. C-band analysis showed that the C-band of chickens, quails, and their hybrids were present on all W-sex chromosomes in all female fission phases and were deeply stained. The combined analysis of the karyotypes and different genotypes of chickens, quails, and their hybrids could provide a reference to accelerate the breeding process.

show abstract

Molecular sex determination of 20 bird species protected in the Republic of Serbia

Cited by 9 publications

References 19 publications

Potential of Inter- and Intra-Species Variability of CHD1 Gene in Birds as a Forensic Tool

Potential of Inter- and Intra-Species Variability of CHD1 Gene in Birds as a Forensic Tool

First Report on Centrorhynchus Aluconis in Common Buzzard (Buteo Buteo) in Northwest Iran

Tavuk, Bıldırcın ve Hibritlerinin Kromozom Karyotipleri ve Bantlanma Modeli Analizi

Contact Info

Product

Resources

About