Sex- and tissue-specific effects of waterborne estrogen on estrogen receptor subtypes and E2-mediated gene expression in the reproductive axis of goldfish

Effects of steroid hormones on reproduction- and detoxification-related gene expression in adult male mosquitofish, Gambusia affinis

“…Moreover, it is interesting to note that the three subtypes of the Vtg gene were also induced in the testis in E2-treated mosquitofish, although not to significant levels in some treatments, which may be due to the relatively great variation that was found among replicates. Nevertheless, previous studies have shown that E2 induced an active transcription of Vtg gene in the testis of fish (Wang et al, 2005;Marlatt et al, 2010;Del Giudice et al, 2011). In the present study, P and T stimulated Vtg mRNA expression levels in the liver and testis of mosquitofish, but not to a significant level, which is in agreement with the results of Mori et al (1998), who reported that not only high but also low concentrations of P and T could stimulate Vtg gene expression in immature male rainbow trout hepatocytes.…”

“…The presence of these steroid hormones may affect aquatic organisms (Miracle et al, 2006;Davis et al, 2007;Milla et al, 2011;Zucchi et al, 2012). Up to now, most studies in the literature focus on the biological effects caused by estrogens (Teles et al, 2006;Carrera et al, 2007;Davis et al, 2007;Henry et al, 2009;Marlatt et al, 2010;Del Giudice et al, 2011), with relatively few studies having examined the biological effects of other steroid hormones such as androgens and progestins.…”

Hormonal effects of tetrabromobisphenol A using a combination of in vitro and in vivo assays

“…This is in agreement with the previously reported ER-dependent accumulation of Vtg mRNA (Huang et al, 2012). However, our results have some discrepancies with several previous studies in teleosts including mosquitofish showing that hepatic ERα is most involved in the transcriptional regulation of Vtg genes (Esterhuyse et al, 2010;Marlatt et al, 2010;Huang et al, 2012). In addition, only ERβ mRNA expression was found in the testis of mosquitofish exposed to 50 nM TBBPA preceding significant induction of Vtg mRNA expression, suggesting a key role of ERβ in controlling Vtg mRNA expression in the testis.…”

Regulation of reproduction- and biomarker-related gene expression by sex steroids in the livers and ovaries of adult female western mosquitofish (Gambusia affinis)

“…Many previous studies in teleosts showed that the expression of hepatic ERα mRNA is significantly induced by E2 treatment (Sabo-Attwood et al, 2004;Esterhuyse et al, 2010;Marlatt et al, 2010). In contrast to ERα, ERβ transcriptional regulation by estrogen seems to be more variable depending on the species and ERβ subtypes (Sabo-Attwood et al, 2004;Pinto et al, 2006;Esterhuyse et al, 2010).…”