Sex and organ differences in oxidative DNA and RNA damage due to treatment of Sprague-Dawley rats with acetoxime or 2-nitropropane

Nie, Guoxing; Conaway, C. Clifford; Hussain, Nalband; Fiala, Emerich S.

doi:10.1093/carcin/11.9.1659

Cited by 31 publications

(23 citation statements)

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“…( 17 ) and Pilger et al ., ( 18 ) and is compatible with the results of an animal study showing that female rats produce less 8‐OH‐dG in liver nucleic acids than male rats when treated with carcinogens. ( 30 ) A negative correlation between BMI and urinary 8‐OH‐dG level is consistent with the findings of Loft et al . ( 17 ) and ourselves, ( 20 ) but is inconsistent with those of Pilger et al .…”

Section: Discussionsupporting

confidence: 92%

Occupational and lifestyle factors and urinary 8‐hydroxydeoxyguanosine

et al. 2005

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The amount of 8-hydroxydeoxyguanosine (8-OH-dG) excreted in urine can be used not only as an indicator of DNA repair capacity, but also as a potential marker of oxidative DNA damage. To clarify the oxidation-related factors, in consideration of cancer risk, this study investigated how urinary 8-OH-dG was associated with occupational and lifestyle factors in 372 healthy workers. The creatinine-adjusted urinary 8-OH-dG level was significantly higher in male subjects, smokers and drinkers compared with their counterparts. There were significant positive correlations of the 8-OH-dG level with average number of working hours, involvement in work, average number of cigarettes smoked, average volume of alcohol consumed and serum cortisol level, and there were significant negative correlations of the 8-OH-dG level with body mass index (BMI) and consumption of soybean products, rice and light-colored vegetables. Multiple regression analysis showed that average number of working hours and average number of cigarettes smoked were significant predictors of increased 8-OH-dG levels, whereas being female and BMI were significant predictors of decreased 8-OH-dG levels. Working hours, BMI and smoking were significant predictors of urinary 8-OH-dG in male subjects, whereas age and BMI were significant predictors in female subjects. We suggest that several occupational and lifestyle factors, particularly long working hours and cigarette smoking, are linked to the formation of 8-OH-dG in workers. (Cancer Sci 2005; 96: 600-606) I t is well-known and documented that reactive oxygen species (ROS) of the cellular metabolism can directly lead to oxidative damage to DNA, and can play an important role in various biological phenomena, including mutation, carcinogenesis, inflammation, aging and degenerative diseases.(1) 8-hydroxydeoxyguanosine (8-OH-dG, 7,8-dihydro-8-oxoguanosine) was first reported as causing oxidative DNA damage in 1984.(2) In cellular DNA, 8-OH-dG causes both mutagenesis and carcinogenesis, (3,4) and furthermore, it has been investigated in relation to a variety of diseases and hazards, particularly cancer and its risk factors.(3,4) For example, the formation of 8-OH-dG in various tissues or peripheral leukocytes as a result of administered carcinogens and other hazardous substances has been reported in many studies, (3,4) and increased 8-OHdG has been found in cancerous tissues.(5,6) Activities that have been linked to cancer, such as smoking and drinking, have also been reported to affect leukocyte or tissue 8-OHdG.(7-10) We provided the first evidence of a relationship between psychosocial stress and leukocyte 8-OH-dG levels in healthy workers, although sex differences also had an effect. (11)(12)(13) Taking these findings into consideration, we conclude that 8-OH-dG could be a useful marker for assessing cancer risk due to multiple risk factors.Tissue or leukocyte 8-OH-dG has been most frequently measured using a high performance liquid chromatography and electrochemical detector (HPLC-ECD) system because of its se...

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Section: Discussionsupporting

confidence: 92%

Occupational and lifestyle factors and urinary 8‐hydroxydeoxyguanosine

et al. 2005

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“…[1][2][3] In spite of the positive associations between negative emotions and the 8-OH-dG levels, females may be more resistant to cancer initiation due to oxidative DNA damage than males, since female rats produce less 8-OH-dG than male rats when treated with carcinogens. 48) Several limitations must also be considered in discussing our findings. First, it has been reported that some aspects of lifestyle, such as physical exercise and dietary habits, are also related to the formation of 8-OH-dG, in addition to cigarette smoking and alcohol use.…”

Section: Discussionmentioning

confidence: 95%

Psychosocial Factors as a Potential Trigger of Oxidative DNA Damage in Human Leukocytes

Irie¹,

Asami²,

Shoji³

et al. 2001

Japanese Journal of Cancer Research

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Although numerous studies have been carried out on the stress‐cancer linkage, the results are still inconclusive. One of the useful, but rarely applied, methods to assess this linkage is to examine the relationship between psychosocial stress and cancer‐predisposing genetic alterations simultaneously. We investigated whether various psychosocial factors can be associated with the levels of 8‐hydroxydeoxyguanosine (8‐OH‐dG), a biomarker of cancer‐related oxidative DNA damage, in peripheral blood leukocytes in 362 healthy workers (276 males and 86 females). After adjustments for age, body mass index, cigarette smoking, and alcohol use, female subjects showed positive relationships between the amount of 8‐OH‐dG and the Tension‐Anxiety, Depression‐Rejection, Anger‐Hostility, Fatigue, and Confusion scores of the Profile of Mood States, respectively. The levels of 8‐OH‐dG also increased reliably in the female subjects who had poor stress‐coping behaviors, particularly wishful thinking strategy, in the NIOSH general job stress instrument. There were positive relationships of the 8‐OH‐dG levels to average working hours, a self‐blame coping strategy, and recent loss of a close family member in male subjects. These findings in a nonclinical sample of healthy adults not only provide evidence of a stress‐cancer linkage, but also suggest possible sex differences in the mechanisms of stress‐related cancer initiation.

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“…Consistent with this observation, less RNA and DNA modifications were detected in female rats than in male rats. Furthermore, RNA and DNA modifications were observed in liver, which represents the target tissue for 2-nitropropane [ 60 ], whereas they were very low in nontarget organs [ 63 ] and were minimal in rabbits [ 64 ], characterized by a refractory nature to 2-nitropropane carcinogenic effects [ 65 ].…”

Section: Oxidative Rna Damage In Chronic-degenerative Diseasesmentioning

confidence: 99%

Role of Oxidative RNA Damage in Chronic-Degenerative Diseases

Fimognari

2015

Oxidative Medicine and Cellular Longevity

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Normal cellular metabolism and exposure to ionizing and ultraviolet radiations and exogenous agents produce reactive oxygen species (ROS). Due to their reactivity, they can interact with many critical biomolecules and induce cell damage. The reaction of ROS with free nucleobases, nucleosides, nucleotides, or oligonucleotides can generate numerous distinct modifications in nucleic acids. Oxidative damage to DNA has been widely investigated and is strongly implicated in the development of many chronic-degenerative diseases. In contrast, RNA damage is a poorly examined field in biomedical research. In this review, I discuss the importance of RNA as a target of oxidative damage and the role of oxidative damage to RNA in the pathogenesis of some chronic-degenerative diseases, such as neurological disorders, atherosclerosis, and cancer. Furthermore, I review recent evidence suggesting that RNA may be the target for toxic agents and indicating RNA degradation as a powerful tool to treat any pathology in which there is an aberrant expression of mRNA and/or its gene products.

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Sex and organ differences in oxidative DNA and RNA damage due to treatment of Sprague-Dawley rats with acetoxime or 2-nitropropane

Cited by 31 publications

References 0 publications

Occupational and lifestyle factors and urinary 8‐hydroxydeoxyguanosine

Occupational and lifestyle factors and urinary 8‐hydroxydeoxyguanosine

Psychosocial Factors as a Potential Trigger of Oxidative DNA Damage in Human Leukocytes

Role of Oxidative RNA Damage in Chronic-Degenerative Diseases

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