Sex affects immunolabeling for histone 3 K27me3 in the trophectoderm of the bovine blastocyst but not labeling for histone 3 K18ac

Carvalheira, Luciano de Rezende; Tríbulo, Paula; Borges, Álan Maia; Hansen, Peter J.

doi:10.1371/journal.pone.0223570

Cited by 9 publications

(2 citation statements)

References 39 publications

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“…The X-chromosome bias of the SDMT and identification of factors related with DNA methylation machinery, reproduction, meiotic cell cycle and gamete generation strengthens the link between sexual transcriptomic and epigenetic dimorphism in blastocysts. Moreover, other epigenetic marks such as histone modifications have revealed epigenetic dimorphism recently, as it can be modified in the trophoctoderm by embryo sex [71]. Furthermore, there are other organisms in nature, as aphids, which in the absence of confounding genetic variation, have revealed that methylation regulates phenotypic plasticity and is intrinsically linked to sexual dimorphism [72].…”

Section: Discussionmentioning

confidence: 99%

Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos

Cánovas

Ivanova

Hamdi

et al. 2021

IJMS

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Assisted reproductive technologies impact transcriptome and epigenome of embryos and can result in long-term phenotypic consequences. Whole-genome DNA methylation profiles from individual bovine blastocysts in vivo- and in vitro-derived (using three sources of protein: reproductive fluids, blood serum and bovine serum albumin) were generated. The impact of in vitro culture on DNA methylation was analyzed, and sex-specific methylation differences at blastocyst stage were uncovered. In vivo embryos showed the highest levels of methylation (29.5%), close to those produced in vitro with serum, whilst embryos produced in vitro with reproductive fluids or albumin showed less global methylation (25–25.4%). During repetitive element analysis, the serum group was the most affected. DNA methylation differences between in vivo and in vitro groups were more frequent in the first intron than in CpGi in promoters. Moreover, hierarchical cluster analysis showed that sex produced a stronger bias in the results than embryo origin. For each group, distance between male and female embryos varied, with in vivo blastocyst showing a lesser distance. Between the sexually dimorphic methylated tiles, which were biased to X-chromosome, critical factors for reproduction, developmental process, cell proliferation and DNA methylation machinery were included. These results support the idea that blastocysts show sexually-dimorphic DNA methylation patterns, and the known picture about the blastocyst methylome should be reconsidered.

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Section: Discussionmentioning

confidence: 99%

Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos

Cánovas

Ivanova

Hamdi

et al. 2021

IJMS

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“…Another important determinant of the function of the preimplantation embryo is embryo sex. In cattle, for example, male blastocysts differ from females in terms of gene expression, mitochondrial DNA content, telomere length, DNA methylation, histone methylation and secretion of the signaling molecule, interferon-τ ( Siqueira and Hansen 2016 ; Bermejo-Alvarez et al 2010 ; Bermejo-Alvarez et al 2008 ; Larson et al 2001 ; Carvalheira et al 2019 ). Female embryos have been reported to develop slower, undergo more apoptosis and have lower cell number than male embryos ( Ghys et al 2015 ; Oliveira et al 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Determinants of survival of the bovine blastocyst to cryopreservation stress: treatment with colony stimulating factor 2 during the morula-to-blastocyst transition and embryo sex

et al. 2020

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Background: Colony-stimulating factor 2 (CSF2) is an important maternal regulator of embryonic development. Earlier research indicates that CSF2 can regulate genes involved in cellular stress responses and block apoptosis. Here, we tested whether addition of 10 ng/mL CSF2 at day 5 of development would increase the survival of blastocysts harvested at day 7 and subjected to vitrification. Additional objectives were to determine whether embryo sex affected survival or whether effects of CSF2 interacted with sex. Results: Survival after vitrification was measured as the percent of warmed blastocysts that re-established a blastocoele after culture and that underwent hatching from the zona pellucida. In the first experiment, blastocysts were vitrified, warmed, cultured for 24 h, and DNA embryo sexing performed by PCR. There was no effect of CSF2, sex, or the interaction on the percent of blastocysts that re-expanded or that were hatching or hatched. In the second experiment, vitrified blastocysts were warmed and cultured for 24, 48, and 72 h. Treatment with CSF2 increased (P = 0.021) the percent of blastocysts that re-expanded as compared to the vehicle group (overall, 77.8 ± 4.7% vs 73.3 ± 4.7%). Percent re-expansion was highest at 24 h and declined slightly thereafter (P = 0.024). Although the interaction was not significant, the effect of CSF2 was greater at 48 and 72 h than at 24 h because CSF2 reduced the incidence of embryos collapsing after re-expansion. Furthermore, the proportion of re-expanded blastocysts at 24 h that experienced blastocoel collapse by 72 h was lower (P = 0.053) for CSF2 (3.6%; 7/195) than for vehicle (8.2%; 16/195). The percent of warmed blastocysts that were hatching or hatched increased with time (P < 0.0001) but there was no effect of CSF2 or the interaction with time on hatching. Conclusion: Treatment with CSF2 from day 5 to 7 of development did not cause a significant effect on the percent of blastocysts that re-established the blastocoele after 24 h of culture but CSF2 reduced the collapse of the blastocoele that occurred for a portion of the embryos that had experienced re-expansion at 24 h. Thus, CSF2 can provide protection to a proportion of blastocysts from cryodamage caused by vitrification. Further work is needed to evaluate whether CSF2 increases survival of vitrified blastocysts after embryo transfer.

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Epigenetic Bearing on Fertility in Farm Animals

Datta¹,

Kumar²,

Verma³

et al. 2022

Current Concepts in Bovine Reproduction

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Sex affects immunolabeling for histone 3 K27me3 in the trophectoderm of the bovine blastocyst but not labeling for histone 3 K18ac

Cited by 9 publications

References 39 publications

Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos

Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos

Determinants of survival of the bovine blastocyst to cryopreservation stress: treatment with colony stimulating factor 2 during the morula-to-blastocyst transition and embryo sex

Epigenetic Bearing on Fertility in Farm Animals

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