Severe sepsis and diabetes mellitus have additive effects on red blood cell deformability

Moutzouri, Antonia G.; Athanassiou, G.; Dimitropoulou, Dimitra; Skoutelis, Athanassios; Gogos, Charalambos

doi:10.1016/j.jinf.2008.04.004

Cited by 33 publications

(23 citation statements)

References 21 publications

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“…Septic shock and multiple organ dysfunction syndrome (MODS) are the most severe outcomes in patients who develop sepsis [1,2]. Additionally, sepsis and septic shock are common causes of death in intensive care units (ICU), particularly affecting elderly, diabetic, immunocompromised, and seriously ill patients [3,4]. Studies in humans have shown that diabetes mellitus (DM) causes immune dysfunction and reduces secretion of pro-inflammatory cytokines and chemokines such as interleukin (IL)-8, which may explain the susceptibility to infections observed in patients with DM [5,6].…”

Section: Introductionmentioning

confidence: 99%

Insulin Modulates Liver Function in a Type I Diabetes Rat Model

Nolasco¹,

Zanoni²,

Nunes³

et al. 2015

Cell Physiol Biochem

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Background/Aims: Several studies have been performed to unravel the association between diabetes and increased susceptibility to infection. This study aimed to investigate the effect of insulin on the local environment after cecal ligation and puncture (CLP) in rats. Methods: Diabetic (alloxan, 42 mg/kg i.v., 10 days) and non-diabetic (control) male Wistar rats were subjected to a two-puncture CLP procedure and 6 h later, the following analyses were performed: (a) total and differential cell counts in peritoneal lavage (PeL) and bronchoalveolar lavage (BAL) fluids; (b) quantification of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-10 and cytokine-induced neutrophil chemoattractant (CINC)-1 and CINC-2 in the PeL and BAL fluids by enzyme-linked immunosorbent assay (ELISA); (c) total leukocyte count using a veterinary hematology analyzer and differential leukocyte counts on stained slides; (d) biochemical parameters (urea, creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) by colorimetric analyses); and (e) lung, kidney, and liver morphological analyses (hematoxylin and eosin staining). Results: Relative to controls, non-diabetic and diabetic CLP rats exhibited an increased in the concentration of IL-1β, IL-6, IL-10, CINC-1, and CINC-2 and total and neutrophil in the PeL fluid. Treatment of these animals with neutral protamine Hagedorn insulin (NPH, 1IU and 4IU, respectively, s.c.), 2 hours before CLP procedure, induced an increase on these cells in the PeL fluid but it did not change cytokine levels. The levels of ALT, AST, ALP, and urea were higher in diabetic CLP rats than in non-diabetic CLP rats. ALP levels were higher in diabetic sham rats than in non-diabetic sham rats. Treatment of diabetic rats with insulin completely restored ALT, AST, and ALP levels. Conclusion: These results together suggest that insulin attenuates liver dysfunction during early two-puncture CLP-induced peritoneal inflammation in diabetic rats.

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Section: Introductionmentioning

confidence: 99%

Insulin Modulates Liver Function in a Type I Diabetes Rat Model

Nolasco¹,

Zanoni²,

Nunes³

et al. 2015

Cell Physiol Biochem

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“…High blood pressure causes elevated hematocrit, plasma viscosity and fibrinogen (Ajmani, 1997;Bogar, 2002;Cicco et al, 1999;Hoieggen et al, 2003;Klein et al, 1995). Severe septic events include misdistribution of blood flow and marked disturbances of microvascular flow leading to tissue hypoperfusion and impaired aggregation and deformability of RBC (Baskurt et al, 1997;Bateman et al, 2003;Condon et al, 2007;Kirschenbaum et al, 2000;Moutzouri et al, 2008;Piagnerelli et al, 2003a;Piagnerelli et al, 2003b;Voerman et al, 1989). Increased aggregation and deformability of RBC is also seen in cerebral and myocardial ischemic conditions as well (Bhavsar & Rosenson, 2010;Bolokadze et al, 2006;Francis, 1991;Huang et al, 1998a;Huang et al, 1998b;Kuke et al, 2001;McHedlishvili et al, 2004).…”

Section: Rheological Parameters and Pathological Conditionsmentioning

confidence: 99%

How Ozone Treatment Affects Erythrocytes

Aydoğan¹,

Artis²

2012

Hemodynamics - New Diagnostic and Therapeutic Approaches

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“…The abnormalities associated with each of these parameters have been shown to markedly increase both plasma and whole blood viscosity (WBV) (Cho et al, 2008). Studies have shown that vascular damage is one of the major characteristic of diabetes (Elishkevitz et al, 2002), which is accompanied by rheological abnormalities that can cause hyperviscosity syndrome (Moutzouri et al, 2008), that is, inadequate metabolic control associated with changing values of blood viscosity. Studies however have also shown that long term diabetes mellitus is associated with increased whole blood viscosity [Vigilance and Reid, 2005;Kaymaz et al, 2005) and decreased haematocrit (Thomas et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of sleep deprivation on hemorheological properties in alloxan induced diabetic rats

Oluranti¹,

N.E²,

A.A³

2017

EJMR

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Diabetes mellitus with abnormal glucose concentration is associated with changes in hemorheological properties, endothelial function, and platelets hyperactivity. Disturbances may significantly be responsible for diabetes-related vascular complications. People with diabetes are also more likely to have sleep problem/poor sleep. This study therefore investigated the hemorheologic effect of sleep deprivation on alloxan induced diabetic rats. Sixteen healthy male Wistar rats were used for the study randomly divided into non diabetic non sleep deprived, diabetic non sleep deprived, non diabetic sleep deprived and diabetic sleep deprived groups with four animals each. The animals were paradoxically sleep deprived for fourteen days with a sleep deprivation chamber. The result revealed an increase in whole blood viscosity, hematocrit, fibrinogen concentration and relative plasma viscosity in diabetic rats. However, these blood rheological properties decline in sleep deprived diabetic rats. This suggests that diabetes increase hemorheological indices which could lead to vascular complications; while sleep deprivation in normal and diabetic state reduces the hemorheological indices. It is therefore possible that paradoxical sleep deprivation did not synergistically with diabetic condition to alter blood rheological properties but otherwise.

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Severe sepsis and diabetes mellitus have additive effects on red blood cell deformability

Cited by 33 publications

References 21 publications

Insulin Modulates Liver Function in a Type I Diabetes Rat Model

Insulin Modulates Liver Function in a Type I Diabetes Rat Model

How Ozone Treatment Affects Erythrocytes

Effect of sleep deprivation on hemorheological properties in alloxan induced diabetic rats

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