Seventy years of the Chagas disease Bambui Project: celebration and perspectives

Dias, João Carlos Pinto; Matos, Christiane Santos

doi:10.1590/0037-8682-0175-2013

Cited by 3 publications

(2 citation statements)

References 4 publications

(13 reference statements)

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“…The Bambui cohort included in this study presented an overall seroprevalence of 37.5% for T. cruzi infection, including 35.6% for patients aged 60 to 69 years old, and 40.5% for both patients aged 70 to 79 and 80+ years old. According to previous studies carried out in the area of Bambui city, TcII is the predominant genotype being found in approximately 77.6% of T. cruzi infected patients [23].…”

Section: Study Design and Populationmentioning

confidence: 91%

CXCL9 and CXCL10 display an age-dependent profile in Chagas patients: a cohort study of aging in Bambui, Brazil

et al. 2020

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Background Chagas disease is endemic in Latin America and still represents an important public health problem in the region. Chronic cardiomyopathy is the most significant chronic form due to its association with morbidity and mortality. The last decade has seen increasing evidence that inflammatory cytokines and chemokines are responsible for the generation of inflammatory infiltrate and tissue damage, with chronic chagasic cardiomyopathy patients presenting a pro-inflammatory immune response. Although studies have evaluated the role of chemokines in experimental T. cruzi infection, few have addressed their systemic profile, especially for human infection and in aging populations. The present work aimed to use the data from a large population based study of older adults, conducted in an endemic area for Chagas disease, to examine the association between serum levels of cytokines and chemokines, T. cruzi infection and electrocardiogram (ECG) abnormality. Methods The present work evaluated serum levels of CCL2, CXCL9, CXCL10, CCL5, CXCL8, IL-1β, IL-6, TNF, IL-12 and IL-10 by Flow Cytometric Bead Array assay (CBA) and the results expressed in pg/ml. The baseline survey started in January 1st 1997, with 1284 participants of an aged population-based cohort. Participants signed an informed consent at baseline and at each subsequent visit and authorized death certificate and medical records verification. Results Our results demonstrated that Chagas disease patients had higher serum levels of CXCL9, CXCL10 and IL-1β and lower serum levels of CCL5 than non-infected subjects. Moreover, our data demonstrated that CXCL9 and CXCL10 increased in an age-dependent profile in Chagas disease patients. Conclusion Together, this study provided evidences that serum biomarkers increase along the age continuum and may have potential implications for establishing clinical management protocols and therapeutic intervention in Chagas disease patients.

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Section: Study Design and Populationmentioning

confidence: 91%

CXCL9 and CXCL10 display an age-dependent profile in Chagas patients: a cohort study of aging in Bambui, Brazil

et al. 2020

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“…The serum samples were obtained from biorepositories maintained under responsibility of our group (JDR, ML and OAM-F). The genotyping profiles of those samples have been present elsewhere in original publications, including: TcII 31 , TcVI and TcII 20 and TcI 32 – 34 . The serum samples from each participant was inactivated at 56 °C for 30 min and stored in aliquots at − 80 °C until use for Human Chagas-Flow ATE-IgG1 assay.…”

Section: Methodsmentioning

confidence: 99%

Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease

Alessio

Araújo

Silva

et al. 2020

Sci Rep

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The molecular and serological methods available for Discrete Typing Units (DTU)-specific diagnosis of Trypanosoma cruzi in chronic chagas disease present limitations. the study evaluated the performance of Human Chagas-Flow ATE-IgG1 for universal and DTU-specific diagnosis of Chagas disease. A total of 102 sera from Chagas disease patients (CH) chronically infected with TcI, TcVI or TcII DTUs were tested for IgG1 reactivity to amastigote/(A), trypomastigote/(T) and epimastigote/(E) antigens along the titration curve (1:250-1:32,000). The results demonstrated that "AI 250/40%", "EVI 250/30%", "AII 250/40%", "TII 250/40%" and "EII 250/30%" have outstanding accuracy (100%) to segregate CH from non-infected controls. The attributes "TI 4,000/50%", "EI 2,000/50%", "AVI 8,000/60%" and "TVI 4,000/50%" were selected for DTU-specific serotyping of Chagas disease. The isolated use of "EI 2,000/50%" provided the highest co-positivity for TcI patients (91%). The combined decision tree algorithms using the pre-defined sets of attributes showed outstanding full accuracy (92% and 97%) to discriminate "TcI vs TcVI vs TcII" and "TcI vs TcII" prototypes, respectively. The elevated performance of Human Chagas-Flow ATE-IgG1 qualifies its use for universal and TcI/TcVI/TcII-specific diagnosis of Chagas disease. These findings further support the application of this method in epidemiological surveys, post-therapeutic monitoring and clinical outcome follow-ups for Chagas disease. Chagas disease, caused by the parasite Trypanosoma cruzi, affects 8 million people worldwide mainly in Latin America 1. The short-term acute phase of the disease evolves to long-lasting chronic phase with distinct clinical manifestations ranging from asymptomatic to cardiac, digestive or cardiac/digestive clinical forms 2-4. Trypanosoma cruzi presents a remarkable genetic diversity and has been classified into at least six Discrete Typing Units (DTUs) and an emerging DTU named TcBat 5. Several studies have shown that besides selective

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Epidemiology of concurrent Chagas disease and ischemic stroke in a population attending a multicenter quaternary rehabilitation network in Brazil

et al. 2019

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Seventy years of the Chagas disease Bambui Project: celebration and perspectives

Abstract: Revista da Sociedade Brasileira de Medicina Tropical 46(5):533-535, Sep-Oct, 2013http://dx

Cited by 3 publications

References 4 publications

CXCL9 and CXCL10 display an age-dependent profile in Chagas patients: a cohort study of aging in Bambui, Brazil

CXCL9 and CXCL10 display an age-dependent profile in Chagas patients: a cohort study of aging in Bambui, Brazil

Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease

Epidemiology of concurrent Chagas disease and ischemic stroke in a population attending a multicenter quaternary rehabilitation network in Brazil

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