Seven‐day storage of apheresis platelets: report of an in vitro study

Dumont, Larry J.; VandenBroeke, Tania

doi:10.1046/j.1537-2995.2003.00286.x

Cited by 52 publications

(62 citation statements)

References 29 publications

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“…Thirdly, in order to mimic the proper clinical setting, we first used increasing amounts of blood-banked platelet concentrates in order to evaluate platelet recovery. However, in accordance with previously published reports, we observed an important decrease in platelet aggregation ability during the storage time in the platelet concentrate form [39] mainly as a result of metabolic changes in platelets maintained in that medium [40] which prevented us from continuing with this approach. However, it has recently been published that decreased platelet aggregation in stored platelet concentrate improves in the body after transfusion, and transfused platelets have similar aggregation ability compared with the platelets derived from the patient [41], so we used a pool of platelets donated directly from five healthy volunteers.…”

Section: Discussionsupporting

confidence: 92%

Normalization of platelet reactivity in clopidogrel‐treated subjects

Vilahur

Choi

Zafar

et al. 2007

Journal of Thrombosis and Haemostasis

169

112

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Background: Aspirin (ASA) + clopidogrel are commonly used in acute coronary syndrome (ACS), but persistent antiplatelet effects may complicate surgery. Methods and Results: To study the possibility of normalizing platelet reactivity after ASA + clopidogrel treatment, 11 healthy subjects received a 325-mg ASA + clopidogrel loading dose (300 or 600 mg dependent on study arm), followed by 81 mg of ASA + 75 mg of clopidogrel daily for 2 days. Platelet reactivity was assessed by light transmittance aggregometry (LTA) [challenged by adenosine diphosphate (ADP), arachidonic acid (AA), collagen, and thrombin receptor activating peptide (TRAP)] and flow cytometry for platelet activation by GPIIb/ IIIa receptor exposure pretreatment, 4 and 72 h postload. To normalize platelet reactivity, increasing amounts of pooled platelets from five untreated volunteers [volunteers (V)-platelet-rich plasma (PRP)] were added ex vivo to the subject's PRP (S-PRP). At both 4 and 72 h, 40% and 50% V-PRP were needed to overcome platelet disaggregation in the 300 or 600 mg arms, respectively, after ADP challenge; an additional 10% V-PRP fully normalized aggregation. Recovery of function was linear with each incremental increase of V-PRP. ADP-induced GPIIb/IIIa activation showed the same pattern as LTA (r ¼ 0.74). Forty percent V-PRP was required to normalize platelet function to AA, collagen, and TRAP. Conclusion: Our results suggest that the pre-operative transfusion of 10 platelet concentrate units (the equivalent of 40% V-PRP) after a 300-mg clopidogrel loading or 12.5 units (50% V-PRP) after a 600 mg loading may adequately reverse clopidogrel-induced platelet disaggregation to facilitate postoperative hemostasis. An additional 2.5 units fully normalized platelet function. The potential clinical implications of our observations could include shorter hospitalizations and reduced bleeding complications. But these observations should be fully explored in an in vivo clinical setting with clopidogrel-treated patients before and after surgery.

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Section: Discussionsupporting

confidence: 92%

Normalization of platelet reactivity in clopidogrel‐treated subjects

Vilahur

Choi

Zafar

et al. 2007

Journal of Thrombosis and Haemostasis

169

112

View full text Add to dashboard Cite

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“…If this observation is due to the technique of cell separation needs further investigation. A previous in vitro study has found impaired function of PC after a storage time exceeding 7 days [32]. Additionally, Schrezenmeier et al mentioned in a recent review article, that several factors might influence the quality of PC such as extent of cell separation (residual leukocytes), plasma content or additive solution which in turn might affect also lipid concentration of PC [33].…”

Section: Discussionmentioning

confidence: 99%

Effects of Blood Products on Inflammatory Response in Endothelial Cells In Vitro

et al. 2012

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Background Transfusing blood products may induce inflammatory reactions within the vascular compartment potentially leading to a systemic inflammatory response. Experiments were designed to assess the inflammatory potential of different blood products in an endothelial cell-based in vitro model and to compare baseline levels of potentially activating substances in transfusion products. Methods The inflammatory response from pre-activated (endotoxin-stimulated) and non-activated endothelial cells as well as neutrophil endothelial transmigration in response to packed red blood cells (PRBC), platelet concentrates (PC) and fresh frozen plasma (FFP) was determined. Baseline inflammatory mediator and lipid concentrations in blood products were evaluated. Results Following incubation with all blood products, an increased inflammatory mediator release from endothelial cells was observed. Platelet concentrates, and to a lesser extent also FFP, caused the most pronounced response, which was accentuated in already pre-stimulated endothelial cells. Inflammatory response of endothelial cells as well as blood product-induced migration of neutrophils through the endothelium was in good agreement with the lipid content of the according blood product. Conclusion Within the group of different blood transfusion products both PC and FFP have a high inflammatory potential with regard to activation of endothelial cells. Inflammation upon blood product exposure is strongly accentuated when endothelial cells are pre-injured. High lipid contents in the respective blood products goes along with an accentuated inflammatory reaction from endothelial cells.

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“…The expression of CD62P on day 0 averaged 1094.7 percent after activation with TRAP-6 and it dropped to 547.3 percent on day 5 after activation with TRAP-6, as compared with the control values. CD62P expression at the platelet surface is one of the most common markers of platelet activation in PCs and there were standardized protocols established to compare the activation of platelets during storage with increased CD62P level over time (37,(278)(279)(280) In the present study, this criterion was fixed as a gold standard for the evaluation of PL-EV levels as a quality control approach.…”

Section: Discussionmentioning

confidence: 99%

Platelet‐derived extracellular vesicles in plateletpheresis concentrates as a quality control approach

et al. 2015

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BACKGROUND Platelet‐derived extracellular vesicles (PL‐EVs) are present in plateletpheresis concentrates (PCs) and may influence the quality of PCs. The aim of the study was to analyze PC‐derived PL‐EVs and to correlate them with standard quality control (QC) variables of PCs and with donor‐specific laboratory variables. STUDY DESIGN AND METHODS PL‐EVs were analyzed by standard as well as advanced high‐sensitivity flow cytometry (FCM) and nanoparticle tracking analysis. A hematology analyzer was applied to the determination of platelet (PLT) count and immature PLT fraction (IPF). Functional capacity of PLTs (CD62P in response to thrombin receptor‐activating peptide 6 activation) was measured by FCM. All in vitro measurements were carried out on Day 0 and on Day 5. Altogether, a total of 42 PC samples, 15 irradiated on Day 0, were investigated. RESULTS Externalization of CD62P, as an indicator of intact PLT function, significantly decreased during in vitro PLT senescence and CD62P expression inversely correlated with increased PL‐EV levels. Interestingly, in fresh PCs a significant correlation was found between PL‐EVs and different hemapheresis instruments, duration of apheresis, and IPF count in peripheral blood of the donor before apheresis. In senescent PCs, the body mass index of donors inversely correlated with the PL‐EV counts. CONCLUSION Loss of PLT function in PCs was associated with increased PL‐EV levels. Shedding of PL‐EVs depends on shear stress influenced by different hemapheresis settings and diverse preanalytical conditions of donors. PL‐EV analysis may stimulate new quality and apheresis strategies for more vital PLTs for transfusion.

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Seven‐day storage of apheresis platelets: report of an in vitro study

Cited by 52 publications

References 29 publications

Normalization of platelet reactivity in clopidogrel‐treated subjects

Normalization of platelet reactivity in clopidogrel‐treated subjects

Effects of Blood Products on Inflammatory Response in Endothelial Cells In Vitro

Platelet‐derived extracellular vesicles in plateletpheresis concentrates as a quality control approach

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