Set up of a serum-free culture system for bovine embryos: Embryo development and quality before and after transient transfer

George, Fabienne; Daniaux, Christel; Genicot, G.; Verhaeghe, Benjamin; Lambert, Philippe; Donnay, Isabelle

doi:10.1016/j.theriogenology.2007.11.008

Cited by 58 publications

(54 citation statements)

References 68 publications

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“…It has been suggested that BSA can bind many low-molecular-weight compounds of varied origins, including heavy metal ions, free radicals, citrate and steroids (George et al, 2008). Particularly, serum albumin exerts important antioxidant activities in which the molecule acts through its multiple binding sites and free radical-trapping properties (Roche et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin

Yasmin

Otoi

Setiadi

et al. 2015

Acta Veterinaria Hungarica

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Sericin is a water-soluble component of silk and has been used as a biomaterial due to its antibacterial and ultraviolet radiation-resistant properties. This study was designed to evaluate the effect of sericin supplementation in a maturation medium on the meiotic competence and fertilisability of sheep oocytes. Cumulus-oocyte complexes (COCs) were cultured in TCM199 supplemented with sericin at various concentrations of 0 (control), 0.1, 0.25 and 0.5%, either with or without bovine serum albumin (BSA). When the COCs were matured without BSA, the supplementation of 0.1% sericin significantly increased the rates of maturation to metaphase II and the total fertilisation of oocytes compared with the other concentrations of sericin. When the COCs were matured with BSA, the beneficial effects of 0.1% sericin supplementation on the maturation and fertilisation of oocytes were not observed. Our findings indicate that supplementation with 0.1% sericin during maturation culture may improve the nuclear maturation and fertilisability of sheep oocytes. Moreover, it may be possible to replace BSA with sericin in chemically defined media without the risk of disease transmission.

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Section: Discussionmentioning

confidence: 99%

Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin

Yasmin

Otoi

Setiadi

et al. 2015

Acta Veterinaria Hungarica

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“…Based on differential cell staining of fresh blastocysts, we inferred that ICSI-derived expanding blastocysts and their IVF-derived counterparts were of comparable quality (in total cell number and the ICM ratio); therefore, differences in embryo quality before vitrification apparently did not account for the higher sensitivity of ICSI-derived expanding blastocysts to MVC vitrification. The relationship between the size of blastocysts and their cryotolerance has been investigated; larger blastocysts tolerated cryopreservation better than the smaller ones [9,25,29,30], with the exception of one report in cattle [3]. For speciesspecific reasons, there was opposite size-dependent cryotolerance in human [31] and horse [32] blastocysts.…”

Section: Discussionmentioning

confidence: 99%

“…For speciesspecific reasons, there was opposite size-dependent cryotolerance in human [31] and horse [32] blastocysts. It has been reported that tolerance to vitrification of in vitro-produced bovine blastocysts was improved when they were larger than 150 to 160 μm in diameter [6,9,29].…”

Section: Discussionmentioning

confidence: 99%

Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age

et al. 2010

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“…These embryos have a high lipid content in the trophoblast and inner cell mass (ICM) cells [1]; this cytoplasmic accumulation of lipids can affect the cryotolerance of embryos [2]. In some cases, this resulted in large offspring syndrome, characterized by high birth weight, prolonged gestation, frequent dystocia, and elevated abortion rates [3].…”

Section: Introductionmentioning

confidence: 99%

“…This latter culture medium is commonly used as an alternative serum-free culture system [2]. For each part of the study, the media were compared in terms of development and hatching rates.…”

Section: Introductionmentioning

confidence: 99%

In vitro bovine embryo production in a synthetic medium: Embryo development, cryosurvival, and establishment of pregnancy

et al. 2015

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a b s t r a c tThe aim of this study was to develop an in vitro embryo culture medium without either fetal calf serum or BSA, using various growth factors and cytokines (GFs-CYKs; IGF-I, IGF-II, bFGF, LIF, GM-CSF, TGF-b1, and PDGF-BB), and other molecules with surfactant and embryotrophic properties, such as recombinant albumin (RA) and hyaluronan (HA). The first part of the study was dedicated to define the best combination of GFs-CYKs þ RA þ HA for optimal embryonic development. Next, we compared development rates and embryo quality (inner cell mass [ICM]-to-total cell number [TCN] ratio), and postthaw survival and hatching rates using this synthetic medium (T1) and a control medium: synthetic oviduct fluid þ BSA þ ITS (insulin, transferrin, and selenium). The blastocyst rates were significantly higher with T1 than those with the control at 7 and 8 days after fertilization. There was no significant difference in TCN or the ICM/TCN ratio between the two treatments. Survival and hatching rates 48 hours after thawing were similar for both treatments. Finally, nine embryo transfers were conducted using fresh and previously frozen Day-7 blastocysts to evaluate the in vivo viability of embryos produced in this synthetic medium; four gestations were obtained from six fresh embryos and one gestation from three frozen embryos. In conclusion, the fetal calf serum and BSA-free medium, supplemented with GFs-CYKs þ RA þ HA, improved embryo development and gave comparable ICM/TCN ratios and postthaw survival rates to the control with BSA. Fresh and frozen embryos produced in this medium are viable for embryo transfer. This fully synthetic method of embryo culture is a useful means of reducing the risk of disease transmission via embryo transfer.

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Set up of a serum-free culture system for bovine embryos: Embryo development and quality before and after transient transfer

Cited by 58 publications

References 68 publications

Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin

Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin

Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age

In vitro bovine embryo production in a synthetic medium: Embryo development, cryosurvival, and establishment of pregnancy

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