Set-up of a method using LC-UV to assay mometasone furoate in pharmaceutical dosage forms

Ourique, Aline Ferreira; Contri, Renata Vidor; Guterres, Sílvia Stanisçuaski; Beck, Ruy Carlos Ruver; Pohlmann, Adriana Raffin; Melero, Ana; Schaefer, Ulrich F.

doi:10.1590/s0100-40422012000400030

Cited by 14 publications

(5 citation statements)

References 15 publications

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“…LC-UV is widely applied in pharmaceutical analysis for drug assay both in academic laboratories and in the pharmaceutical industry. [33][34][35][36][37][38] The composition of the mobile phase was the rst parameter studied. It was based on the composition described by the USP method, 29 which was also reported by Rieutord and co-workers in 1999.…”

Section: Resultsmentioning

confidence: 99%

A LC-UV method to assay N-acetylcysteine without derivatization: analyses of pharmaceutical products

et al. 2013

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Section: Resultsmentioning

confidence: 99%

A LC-UV method to assay N-acetylcysteine without derivatization: analyses of pharmaceutical products

et al. 2013

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“…The drug was assayed by HPLC according to Ourique et al [25]. The system consisted of a UV detector, pump and autoinjector (S200; PerkinElmer), and a Gemini RP-18 column (150 × 4.60 mm, 5 μm; Phenomenex, Torrance, Calif., USA).…”

Section: Methodsmentioning

confidence: 99%

“…The MF assay in hydrogels was carried out by HPLC after extraction of drug in methanol, according to a previously validated method [25]. Then, 1 g of the formulation (0.5 mg·g -1 of MF) was transferred to a 50-ml volumetric flask (final concentration of 10 µg·ml -1 of the drug), and methanol was added then.…”

Section: Methodsmentioning

confidence: 99%

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Nanoencapsulation in Lipid-Core Nanocapsules Controls Mometasone Furoate Skin Permeability Rate and Its Penetration to the Deeper Skin Layers

Melero

Ourique²,

Guterres³

et al. 2014

Skin Pharmacol Physiol

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Aims: The influence of nanoencapsulation of mometasone furoate (MF) in poly(ε-caprolactone) lipid-core nanocapsules (LNC) on its in vitro human skin permeation and penetration was evaluated. Methods: Semisolid formulations were prepared by increasing the viscosity of LNC using a carbomer (Carbopol® Ultrez at 0.5% w/v). Two complementary techniques (the static Franz diffusion cell model and the Saarbrücken penetration model) were used to evaluate skin permeation/penetration. Results: The drug release rate was decreased by nanoencapsulation. The skin permeability of MF was controlled by the nanoencapsulation as well as by increasing the viscosity. Furthermore, the formulation containing the nanoencapsulated MF controlled the amount of drug reaching the deeper skin layers without changing its accumulation in the stratum corneum. Conclusion: This formulation is suitable for prolonged treatment of skin disorders which should avoid systemic absorption.

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“…Also, MF was determined in its dosage form by HPLC (Ourique et al, 2012). Kinetic study of MF in aqueous system (Teng et al, 2003) and supercritical fluid chromatographic impurity analysis (Wang et al, 2011) were also carried out.…”

Section: Introductionmentioning

confidence: 99%

Forced degradation of mometasone furoate and development of two RP-HPLC methods for its determination with formoterol fumarate or salicylic acid

El‐Bagary

Fouad

El-Shal

et al. 2016

Arabian Journal of Chemistry

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Two simple, selective and precise stability-indicating reversed-phase liquid chromatographic methods were developed and validated for the determination of mometasone furoate in two binary mixtures, with formoterol fumarate (Mixture 1) and salicylic acid (Mixture 2). Also, a forced degradation study of mometasone furoate was carried out including acid and alkali hydrolysis, oxidation, thermal and photo-degradation. For mixture 1, the method was based on isocratic elution using a mobile phase consisting of (Acetonitrile: 3 mM Sodium lauryl sulfate) (60:40, v/v) at a flow rate of 1 ml min À1 . Quantitation was achieved applying dual wavelength detection where mometasone furoate and its degradation products were detected at 247 nm and formoterol fumarate and its degradation product were detected at 214 nm at 30°C. For mixture 2 and for the forced degradation study, separation was based on isocratic elution of mometasone furoate, its degradation products and salicylic acid on a reversed phase C8 column using a mobile phase consisting of acetonitrile:water:methanol:glacial acetic acid (60:30:10:0.1, v/v) at a flow rate of 2 mL min À1 . Quantitation was achieved with UV detection at 240 nm. In addition, products from alkaline forced degradation of mometasone furoate were verified by LC-MS. Linearity, accuracy and precision were found to be acceptable over the concentration range of 10-800 lg mL À1 and 5-60 lg mL À1 for mometasone furoate and formoterol fumarate, respectively and over the concentration range of 5-320 lg mL À1 and 20-1280 lg mL À1 for mometasone furoate and salicylic acid,

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Set-up of a method using LC-UV to assay mometasone furoate in pharmaceutical dosage forms

Cited by 14 publications

References 15 publications

A LC-UV method to assay N-acetylcysteine without derivatization: analyses of pharmaceutical products

A LC-UV method to assay N-acetylcysteine without derivatization: analyses of pharmaceutical products

Nanoencapsulation in Lipid-Core Nanocapsules Controls Mometasone Furoate Skin Permeability Rate and Its Penetration to the Deeper Skin Layers

Forced degradation of mometasone furoate and development of two RP-HPLC methods for its determination with formoterol fumarate or salicylic acid

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