Sesquiterpenoids from Farfugium japonicum and their inhibitory activity on NO production in RAW264.7 cells

Zhao, Jiang-He; Shen, Tong; Xia, Yang; Zhao, Hong; Li, Xia; Xie, Wenrui

doi:10.1007/s12272-012-0705-7

Cited by 28 publications

(13 citation statements)

References 24 publications

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“…However, metabolic activity may be changed by different conditions or chemical treatments which can cause considerable variation in results reported from these assays [21]. Based on our knowledge, seven eremophilane type sesquiterpenes have been reported for their NO production inhibitory activities with the highest activity of 0.55 µg/mL (IC 50 ), and 6-hydroxy group was considered critical for increasing the ability to inhibit NO production [22,23]. In our study, all four compounds (1-4) exhibited nitric oxide inhibitory activities in a dose dependent manner without cytotoxic effects.…”

Section: Discussionmentioning

confidence: 99%

Eremophilane Sesquiterpenes from a Deep Marine-Derived Fungus, Aspergillus sp. SCSIOW2, Cultivated in the Presence of Epigenetic Modifying Agents

Wang

Tang

et al. 2016

Molecules

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Chemical epigenetic manipulation was applied to a deep marine-derived fungus, Aspergillus sp. SCSIOW2, resulting in significant changes of the secondary metabolites. Three new eremophilane-type sesquiterpenes, dihydrobipolaroxin B (2), dihydrobipolaroxin C (3), and dihydrobipolaroxin D (4), along with one known analogue, dihydrobipolaroxin (1), were isolated from the culture treated with a combination of histone deacetylase inhibitor (suberohydroxamic acid) and DNA methyltransferase inhibitor (5-azacytidine). 1-4 were not produced in the untreated cultures. 2 and 3 might be artificial because 1 could form 2 and 3 spontaneously in water by intracellular acetalization reaction. The absolute configurations of 1 and 2 were assigned based on ECD spectroscopy combined with time-dependent density functional theory calculations. All four compounds exhibited moderate nitric oxide inhibitory activities without cytotoxic effects.

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Section: Discussionmentioning

confidence: 99%

Eremophilane Sesquiterpenes from a Deep Marine-Derived Fungus, Aspergillus sp. SCSIOW2, Cultivated in the Presence of Epigenetic Modifying Agents

Wang

Tang

et al. 2016

Molecules

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“…Cytotoxicity of compounds to RAW264.7 cells was tested by MTT assays similar to our previous research. 39 Briefly, the cells were placed at 5 × 10 3 per well in 96-well plates, overnight, and then exposed to tested compounds at given concentrations. DMSO was used as a negative control.…”

Section: Cytotoxicity Assaysmentioning

confidence: 99%

Lignans and Terpenoids From the Fruits of Vitex kwangsiensia and Their Inhibitory Activity on Nitric Oxide Production in Macrophages

Shen

Wang

Zhu

et al. 2019

Natural Product Communications

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Phytochemical investigation of the fruits from Vitex kwangsiensia led to the isolation of 18 constituents, including 6 lignans (1-6), 3 diterpenoids (7-9), 2 sesquiterpenoids (10, 11), 2 triterpenoids (16, 17), and 5 other compounds. Their structures were identified by spectroscopic methods (mass spectroscopy, infrared, UV, 1-dimensional and 2-dimensional nuclear magnetic resonance) and comparison with reported data in the literatures. Among these constituents, compound 1 was a new lignan and named as vitekwangin A. The spectroscopic data of 2 was reported herein for the first time, and given a trivial name vitekwangin B. The isolated lignans, diterpenoids, and sesquiterpenoids were evaluated for their inhibitory activity to prevent nitric oxide production in lipopolysaccharide-stimulated RAW264.7 macrophages.

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“…Accumulation of nitrite, a metabolite of nitric oxide (NO) in the culture media was 2 measured by Griess assay (Zhao et al, 2012).Briefly, cells were plated onto 96-well plates 3 (5×10 4 cell/well) and pretreated with 0-50 μM of LCfor 1h before stimulation with 100 ng/ml (for RAW 264.7) or 1000 ng/ml (for murine peritoneal macrophages)of LPS. After incubation for 24h, an equal volumn (50 μL) of the supernatant was mixed with Griess Reagent in a 96-well plate.…”

Section: Bioassay For No Productionmentioning

confidence: 99%

Licocoumarone isolated from Glycyrrhiza uralensis selectively alters LPS-induced inflammatory responses in RAW 264.7 macrophages

Fan

et al. 2017

European Journal of Pharmacology

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The effects of licocoumarone (LC) isolated from Glycyrrhiza uralensis were studied in LPS-stimulated RAW 264.7 macrophages. Our study demonstrated that LC dose-dependently attenuated LPS-induced NO production by down-regulating iNOS expression. Additionally, the treatment with LC inhibited LPS-induced expression of cytokines including IL-1β, IL-6 and IL-10, but not TNF-α, at both mRNA and protein levels. Similar suppressive effects of LC were observed on LPS-stimulated murine peritoneal macrophages as well. Furthermore, LC significantly reduced LPS-stimulated NF-κB activation by inhibition of IκBα degradation and p65 phosphorylation. The results from NF-κB-luc reporter gene assay further support the inhibitory effect of LC on NF-κB activation. Further studies showed that LC also interfered with the MAPKs and STAT3 signaling pathways, which are typical inflammatory signaling pathways triggered by LPS. Taken together, these results show that LC attenuates LPS-induced cytokine gene expression in RAW 264.7 macrophages through mechanisms that involve NF-κB, MAPKs and STAT3 signaling pathways, but the pattern of inhibition differs from that of a global immunosuppresant. Our study indicates that LC is a functional constituent of Glycyrrhiza uralensis with potential implications in infectious and immune-related diseases.

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Sesquiterpenoids from Farfugium japonicum and their inhibitory activity on NO production in RAW264.7 cells

Cited by 28 publications

References 24 publications

Eremophilane Sesquiterpenes from a Deep Marine-Derived Fungus, Aspergillus sp. SCSIOW2, Cultivated in the Presence of Epigenetic Modifying Agents

Eremophilane Sesquiterpenes from a Deep Marine-Derived Fungus, Aspergillus sp. SCSIOW2, Cultivated in the Presence of Epigenetic Modifying Agents

Lignans and Terpenoids From the Fruits of Vitex kwangsiensia and Their Inhibitory Activity on Nitric Oxide Production in Macrophages

Licocoumarone isolated from Glycyrrhiza uralensis selectively alters LPS-induced inflammatory responses in RAW 264.7 macrophages

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