Sesamin and sesamolin rescues Caenorhabditis elegans from Pseudomonas aeruginosa infection through the attenuation of quorum sensing regulated virulence factors

Anju, V. T.; Siddhardha, Busi; Ranganathan, Sampathkumar; Ampasala, Dinakara Rao; Kumar, Sandeep; Suchiang, Kitlangki; Kumavath, Ranjith; Dyavaiah, Madhu

doi:10.1016/j.micpath.2021.104912

Cited by 33 publications

(18 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to the tight regulation of biofilm and antibiotic resistance by the QS system, efforts have been made to find QS inhibitors or quenchers that can overcome antibiotic resistance and combat biofilm formation [5,[7][8][9][10][11][12][818][819][820][821] (Table 4). QS inhibitors interfere with the action of one or more of the components of the QS system, thereby reducing the virulence and biofilm formation of the bacteria with consequently increased sensitivity to antibiotics.…”

Section: Quorum Sensing Inhibitors and Quenchersmentioning

confidence: 99%

“…In a Pseudomonas aeruginosa infection model in Caenorhabditis elegans, the lignans Sesamin and Sesamolin isolated from the Sesamum indicum (L.) plant were found to prevent the infection of pre-infected worms through attenuation of QS-regulated virulence factors of the bacteria [818]. Both the lignans exerted anti-QS activity at 75 µg/mL without affecting the bacterial growth.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Targeting the Holy Triangle of Quorum Sensing, Biofilm Formation, and Antibiotic Resistance in Pathogenic Bacteria

Sionov

Steinberg

2022

Microorganisms

View full text Add to dashboard Cite

Chronic and recurrent bacterial infections are frequently associated with the formation of biofilms on biotic or abiotic materials that are composed of mono- or multi-species cultures of bacteria/fungi embedded in an extracellular matrix produced by the microorganisms. Biofilm formation is, among others, regulated by quorum sensing (QS) which is an interbacterial communication system usually composed of two-component systems (TCSs) of secreted autoinducer compounds that activate signal transduction pathways through interaction with their respective receptors. Embedded in the biofilms, the bacteria are protected from environmental stress stimuli, and they often show reduced responses to antibiotics, making it difficult to eradicate the bacterial infection. Besides reduced penetration of antibiotics through the intricate structure of the biofilms, the sessile biofilm-embedded bacteria show reduced metabolic activity making them intrinsically less sensitive to antibiotics. Moreover, they frequently express elevated levels of efflux pumps that extrude antibiotics, thereby reducing their intracellular levels. Some efflux pumps are involved in the secretion of QS compounds and biofilm-related materials, besides being important for removing toxic substances from the bacteria. Some efflux pump inhibitors (EPIs) have been shown to both prevent biofilm formation and sensitize the bacteria to antibiotics, suggesting a relationship between these processes. Additionally, QS inhibitors or quenchers may affect antibiotic susceptibility. Thus, targeting elements that regulate QS and biofilm formation might be a promising approach to combat antibiotic-resistant biofilm-related bacterial infections.

show abstract

Section: Quorum Sensing Inhibitors and Quenchersmentioning

confidence: 99%

mentioning

confidence: 99%

Targeting the Holy Triangle of Quorum Sensing, Biofilm Formation, and Antibiotic Resistance in Pathogenic Bacteria

Sionov

Steinberg

2022

Microorganisms

View full text Add to dashboard Cite

show abstract

“…Numerous dietary molecules with diverse structures have been shown to interfere with bacterial virulence, including inhibition of QS [ 91 , 92 , 93 , 94 ]. They can compete with bacterial QS molecules for receptor binding as a result of structural similarities (pyrogallol) [ 95 ], sequester bacterial QS molecules (polyphenols and lignans) [ 96 , 97 ], inhibit QS molecule synthesis (naringenin) [ 98 , 99 ], accelerate QS molecule or receptor degradation (halogenated furanones) [ 100 ], and modulate receptor activity (cinnamaldehyde and luteolin) [ 101 , 102 ]. Additionally, plant products interfere with bacterial membranes and their metabolism leading to a decrease in QS molecule production.…”

Section: Factors Influencing Qs In the Gastrointestinal Tractmentioning

confidence: 99%

Making Sense of Quorum Sensing at the Intestinal Mucosal Interface

Uhlig

Hyland

2022

Cells

View full text Add to dashboard Cite

The gut microbiome can produce metabolic products that exert diverse activities, including effects on the host. Short chain fatty acids and amino acid derivatives have been the focus of many studies, but given the high microbial density in the gastrointestinal tract, other bacterial products such as those released as part of quorum sensing are likely to play an important role for health and disease. In this review, we provide of an overview on quorum sensing (QS) in the gastrointestinal tract and summarise what is known regarding the role of QS molecules such as auto-inducing peptides (AIP) and acyl-homoserine lactones (AHL) from commensal, probiotic, and pathogenic bacteria in intestinal health and disease. QS regulates the expression of numerous genes including biofilm formation, bacteriocin and toxin secretion, and metabolism. QS has also been shown to play an important role in the bacteria–host interaction. We conclude that the mechanisms of action of QS at the intestinal neuro–immune interface need to be further investigated.

show abstract

“…Lignans are reported for their ability to reduce the production of exopolysaccharides important for biofilm formation, in addition to downregulation of QS gene expression in P. aeruginosa. As a consequence, the production of virulence factors and enzymes is suppressed [47]. Phenolics could kill pathogens and inhibit biofilm via different mechanisms: disruption of the bacterial membrane (with subsequent leakage of cellular content) and inhibition of cell-cell adhesion, inhibition of extracellular matrix formation and inhibition of Staphylococcal protein A, thus preventing bacterial adhesion and attachment required for biofilm production [45].…”

Section: Anti-virulent Activity Of the Tested Extractsmentioning

confidence: 99%

The Untargeted Phytochemical Profile of Three Meliaceae Species Related to In Vitro Cytotoxicity and Anti-Virulence Activity against MRSA Isolates

et al. 2022

View full text Add to dashboard Cite

Background: A high mortality rate is associated with about 80% of all infections worldwide, mainly due to antimicrobial resistance. Various antimicrobial and cytotoxic activities have been proposed for Meliaceae species. This study aimed to evaluate the in vitro anti-virulence and cytotoxic effect of the leaf extracts of Aphanamixis polystachya, Toona ciliata and Melia azedarach against five MRSA strains and on three cancer cell lines, followed by biological correlation to their encompassed phytoconstituents. Material and Methods: We explored three plants of this family against a panel of Methicillin-resistant Staphylococcus aureus (MRSA) strains and several cancer cell lines to select the most promising candidates for further in vivo and preclinical studies. The phytochemical composition was evaluated by UHPLC–QTOF–MS untargeted profiling. Cell viability was assessed by SRB assay. Minimum Inhibitory Concentration was carried out by using the agar micro-dilution technique. Inhibition of biofilm formation and preformed biofilm disruption were assessed spectrophotomertically, according to the Sultan and Nabil method (2019). Results: A total of 279 compounds were putatively annotated to include different phytochemical classes, such as flavonoids (108), limonoids/terpenoids (59), phenolic acids (49) and lower-molecular-weight phenolics (39). A. polystachya extract showed the most potent cytotoxic activity against Huh-7, DU-145 and MCF-7 cell lines (IC50 = 3, 3.5 and 13.4 µg mL−1, respectively), followed by M. azedarach, with no effect recorded for T. ciliata extract. Furthermore, both A. polystachya and M. azedarach extracts showed promising anti-virulence and antimicrobial activities, with A. polystachya being particularly active against MRSA. These two latter extracts could inhibit and disrupt the biofilm, formed by MRSA, at sub-lethal concentrations. Interestingly, the extracts inhibited hemolysin-α enzyme, thus protecting rabbit RBCs from lysis. A. polystachya extract reduced the pigmentation and catalase enzyme activity of tested pigmented strains better than M. azedarach at both tested sub-MICs. Consequently, susceptibility of the extract-treated cells to oxidant killing by 200 mM H2O2 increased, leading to faster killing of the cells within 120 min as compared to the extract-non-treated cells, likely due to the lower antioxidant-scavenging activity of cells exhibiting less staphyloxanthin production. Conclusion: These findings suggested that both A. polystachya and M. azedarach natural extracts are rich in bioactive compounds, mainly limonoids, phenolics and oxygenated triterpenoids, which can combat MRSA biofilm infections and could be considered as promising sources of therapeutic cytotoxic, antibiofilm and anti-virulence agents.

show abstract

Sesamin and sesamolin rescues Caenorhabditis elegans from Pseudomonas aeruginosa infection through the attenuation of quorum sensing regulated virulence factors

Cited by 33 publications

References 50 publications

Targeting the Holy Triangle of Quorum Sensing, Biofilm Formation, and Antibiotic Resistance in Pathogenic Bacteria

Targeting the Holy Triangle of Quorum Sensing, Biofilm Formation, and Antibiotic Resistance in Pathogenic Bacteria

Making Sense of Quorum Sensing at the Intestinal Mucosal Interface

The Untargeted Phytochemical Profile of Three Meliaceae Species Related to In Vitro Cytotoxicity and Anti-Virulence Activity against MRSA Isolates

Contact Info

Product

Resources

About