Seryl-tRNA Synthetase Shows a Noncanonical Activity of Upregulating Laccase Transcription in
            <i>Trametes hirsuta</i>
            AH28-2 Exposed to Copper Ion

Gan, Zhiwei; Zhang, Xueping; Li, Mengke; Li, Xing; Zhang, Xinlei; Wang, Chenkai; Xiao, Yazhong; Liu, Juanjuan; Fang, Zemin

doi:10.1128/spectrum.00768-23

Cited by 3 publications

(3 citation statements)

References 65 publications

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“…S4). Despite the lack of change in ThserRS expression levels, serine in C. unicolor 87613 did show the same negative impact on laccase production as TherRS reported in T. hisuta AH28-2 [51]. Hence, it remains a possible way to negatively influence fungal laccase production through the interaction between serine and TherRS (or other TFs).…”

Section: Cysteine and Methionine Metabolism And Its Biomarkers Involv...mentioning

confidence: 85%

“…ferulae [50]. Moreover, Gan and collaborators uncovered a novel role for seryl-tRNA synthetase (TherRS) as a TF in the repression of laccase production through its interaction with the promoter region of the laccase gene in T. hisuta AH28-2 [51]. These findings suggest a potential mechanism by which serine might regulate laccase production through interaction with TFs, such as TherRS.…”

Section: Cysteine and Methionine Metabolism And Its Biomarkers Involv...mentioning

confidence: 96%

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A complex metabolic network and its biomarkers regulate laccase production in white-rot fungus Cerrena unicolor 87613

Zhang,

Qiu,

Qiu

et al. 2024

Microb Cell Fact

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Background White-rot fungi are known to naturally produce high quantities of laccase, which exhibit commendable stability and catalytic efficiency. However, their laccase production does not meet the demands for industrial-scale applications. To address this limitation, it is crucial to optimize the conditions for laccase production. However, the regulatory mechanisms underlying different conditions remain unclear. This knowledge gap hinders the cost-effective application of laccases. Results In this study, we utilized transcriptomic and metabolomic data to investigate a promising laccase producer, Cerrena unicolor 87613, cultivated with fructose as the carbon source. Our comprehensive analysis of differentially expressed genes (DEGs) and differentially abundant metabolites (DAMs) aimed to identify changes in cellular processes that could affect laccase production. As a result, we discovered a complex metabolic network primarily involving carbon metabolism and amino acid metabolism, which exhibited contrasting changes between transcription and metabolic patterns. Within this network, we identified five biomarkers, including succinate, serine, methionine, glutamate and reduced glutathione, that played crucial roles in co-determining laccase production levels. Conclusions Our study proposed a complex metabolic network and identified key biomarkers that determine the production level of laccase in the commercially promising Cerrena unicolor 87613. These findings not only shed light on the regulatory mechanisms of carbon sources in laccase production, but also provide a theoretical foundation for enhancing laccase production through strategic reprogramming of metabolic pathways, especially related to the citrate cycle and specific amino acid metabolism.

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Section: Cysteine and Methionine Metabolism And Its Biomarkers Involv...mentioning

confidence: 85%

Section: Cysteine and Methionine Metabolism And Its Biomarkers Involv...mentioning

confidence: 96%

A complex metabolic network and its biomarkers regulate laccase production in white-rot fungus Cerrena unicolor 87613

Zhang,

Qiu,

Qiu

et al. 2024

Microb Cell Fact

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“…The C-terminal extension specific for vertebrate SerRSs, the unique domain UNE-S, harbors robust NLS that redirects the protein from the cytosol into the nucleus, where it exhibits non-canonical functions unrelated to translation [63]. Interestingly, the SerRS from the fungus Trametes hirsuta also shows nuclear localization and acts as a transcriptional repressor [64]. An evolutionary addition to higher eukaryote cytosolic SerRSs are two insertions (Figure 1).…”

Section: Seryl-trna Synthetasesmentioning

confidence: 99%

Protein–Protein Interactions of Seryl-tRNA Synthetases with Emphasis on Human Counterparts and Their Connection to Health and Disease

Dulic,

Godinic-Mikulcic,

Kekez

et al. 2024

Life

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Seryl-tRNA synthetases (SerRSs), members of the aminoacyl-tRNA synthetase family, interact with diverse proteins, enabling SerRSs to enhance their role in the translation of the genetic message or to perform alternative functions in cellular processes beyond translation. Atypical archaeal SerRS interacts with arginyl-tRNA synthetase and proteins of the ribosomal P-stalk to optimize translation through tRNA channeling. The complex between yeast SerRS and peroxin Pex21p provides a connection between translation and peroxisome function. The partnership between Arabidopsis SerRS and BEN1 indicates a link between translation and brassinosteroid metabolism and may be relevant in plant stress response mechanisms. In Drosophila, the unusual heterodimeric mitochondrial SerRS coordinates mitochondrial translation and replication via interaction with LON protease. Evolutionarily conserved interactions of yeast and human SerRSs with m3C32 tRNA methyltransferases indicate coordination between tRNA modification and aminoacylation in the cytosol and mitochondria. Human cytosolic SerRS is a cellular hub protein connecting translation to vascular development, angiogenesis, lipogenesis, and telomere maintenance. When translocated to the nucleus, SerRS acts as a master negative regulator of VEGFA gene expression. SerRS alone or in complex with YY1 and SIRT2 competes with activating transcription factors NFκB1 and c-Myc, resulting in balanced VEGFA expression important for proper vascular development and angiogenesis. In hypoxia, SerRS phosphorylation diminishes its binding to the VEGFA promoter, while the lack of nutrients triggers SerRS glycosylation, reducing its nuclear localization. Additionally, SerRS binds telomeric DNA and cooperates with the shelterin protein POT1 to regulate telomere length and cellular senescence. As an antitumor and antiangiogenic factor, human cytosolic SerRS appears to be a promising drug target and therapeutic agent for treating cancer, cardiovascular diseases, and possibly obesity and aging.

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Two Zn ₂ Cys ₆ -type transcription factors respond to aromatic compounds and regulate the expression of laccases in the white-rot fungus Trametes hirsuta

Wang,

Zhang,

et al. 2024

Appl Environ Microbiol

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White-rot fungi differentially express laccases when they encounter aromatic compounds. However, the underlying mechanisms are still being explored. Here, proteomics analysis revealed that in addition to increased laccase activity, proteins involved in sphingolipid metabolism and toluene degradation as well as some cytochrome P450s (CYP450s) were differentially expressed and significantly enriched during 48 h of o -toluidine exposure, in Trametes hirsuta AH28-2. Two Zn 2 Cys 6 -type transcription factors (TFs), TH8421 and TH4300, were upregulated. Bioinformatics docking and isothermal titration calorimetry assays showed that each of them could bind directly to o -toluidine and another aromatic monomer, guaiacol. Binding to aromatic compounds promoted the formation of TH8421/TH4300 heterodimers. TH8421 and TH4300 silencing in T. hirsuta AH28-2 led to decreased transcriptional levels and activities of LacA and LacB upon o -toluidine and guaiacol exposure. EMSA and ChIP-qPCR analysis further showed that TH8421 and TH4300 bound directly with the promoter regions of lacA and lacB containing CGG or CCG motifs. Furthermore, the two TFs were involved in direct and positive regulation of the transcription of some CYP450s. Together, TH8421 and TH4300, two key regulators found in T. hirsuta AH28-2, function as heterodimers to simultaneously trigger the expression of downstream laccases and intracellular enzymes. Monomeric aromatic compounds act as ligands to promote heterodimer formation and enhance the transcriptional activities of the two TFs. IMPORTANCE White-rot fungi differentially express laccase isoenzymes when exposed to aromatic compounds. Clarification of the molecular mechanisms underlying differential laccase expression is essential to elucidate how white-rot fungi respond to the environment. Our study shows that two Zn 2 Cys 6 -type transcription factors form heterodimers, interact with the promoters of laccase genes, and positively regulate laccase transcription in Trametes hirsuta AH28-2. Aromatic monomer addition induces faster heterodimer formation and rate of activity. These findings not only identify two new transcription factors involved in fungal laccase transcription but also deepen our understanding of the mechanisms underlying the response to aromatics exposure in white-rot fungi.

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Seryl-tRNA Synthetase Shows a Noncanonical Activity of Upregulating Laccase Transcription in Trametes hirsuta AH28-2 Exposed to Copper Ion

Abstract: Seryl-tRNA synthetase is well known for the attachment of serine to the corresponding cognate tRNA during protein translation. In contrast, its functions beyond translation in microorganisms are underexplored.

Cited by 3 publications

References 65 publications

A complex metabolic network and its biomarkers regulate laccase production in white-rot fungus Cerrena unicolor 87613

A complex metabolic network and its biomarkers regulate laccase production in white-rot fungus Cerrena unicolor 87613

Protein–Protein Interactions of Seryl-tRNA Synthetases with Emphasis on Human Counterparts and Their Connection to Health and Disease

Two Zn ₂ Cys ₆ -type transcription factors respond to aromatic compounds and regulate the expression of laccases in the white-rot fungus Trametes hirsuta

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Seryl-tRNA Synthetase Shows a Noncanonical Activity of Upregulating Laccase Transcription in Trametes hirsuta AH28-2 Exposed to Copper Ion

Abstract: Seryl-tRNA synthetase is well known for the attachment of serine to the corresponding cognate tRNA during protein translation. In contrast, its functions beyond translation in microorganisms are underexplored.

Cited by 3 publications

References 65 publications

A complex metabolic network and its biomarkers regulate laccase production in white-rot fungus Cerrena unicolor 87613

A complex metabolic network and its biomarkers regulate laccase production in white-rot fungus Cerrena unicolor 87613

Protein–Protein Interactions of Seryl-tRNA Synthetases with Emphasis on Human Counterparts and Their Connection to Health and Disease

Two Zn 2 Cys 6 -type transcription factors respond to aromatic compounds and regulate the expression of laccases in the white-rot fungus Trametes hirsuta

Contact Info

Product

Resources

About

Two Zn ₂ Cys ₆ -type transcription factors respond to aromatic compounds and regulate the expression of laccases in the white-rot fungus Trametes hirsuta