Serumfreie Kultivierung von Osteoprogenitorzellen und Osteoblasten zur Testung von Biomaterialien

Abstract

The aim of the study is to describe a model for testing biocompatibility of implant materials. Usually cells do not bind the biomaterial surface itself via integrins but adsorbed proteins of blood or interstitial fluids. To eliminate the influence of serum proteins on cell adhesion to the test materials we cultivated osteoprogenitor cells and osteoblasts with a serum replacement or with fetal calf serum, but seeded them likewise without serum or serum replacement on cell culture polystyrene, sandblasted titanium and titanium coated with the peptide c(RGDfK) or hydroxyapatite (Bonemaster) and determined cell adhesion. In addition, the surfaces were preincubated with the serum proteins albumin, fetuin, fibronectin and vitronectin to examine specifically their influence on cell adhesion. Clearly cell adhesion depended on cell culture conditions and state of differentiation, especially with prominent differences in adhesion to c(RGDfK). Precoating with serum proteins demonstrated that besides fibronectin and vitronectin fetuin can function as an adhesion protein, whereas albumin demonstrated an antiadhesive effect. Depending on the material they affected cell adhesion differently. Although osteoprogenitor cells and osteoblasts could bind to tissue culture polystyrene, titanium and especially hydroxyapatite without mediation of proteins, it has to be taken into consideration that cell spreading and proliferation of cells on a scaffold are more important than adhesion alone and may not be ensured in the absence of adhesion proteins.

Derivatization of Plasma Polymerized Thin Films and Attachment of Biomolecules to Influence HUVEC‐Cell Adhesion

“…Beside their importance in cell–cell interactions, the other major function of membrane integrins is their contribution toward the anchoring of the cell to the proteins of the extracellular matrix. The interactions are based on short peptide sequences that are constituents of many proteins of the extra cellular matrix and that interact with the membrane integrins 2, 3. This short sequence is found in proteins such as fibronectin, vitronectin, collagen, and osteopontin and is typically denoted the short form RGD.…”

In Vitro Investigation on Degradable Mg-Based Biomaterial under the Impact of the Serum Glycoprotein Fetuin

“…Therefore, a serum-free pOB culture was generated, which provided a continuous, acceptable viability of more than 85%. A serum-free osteoblast progenitor cell model for biomaterial testing was introduced by Duewelhenke and Eysel (2007) [ 38 ]. It has been shown that fetuin, likewise to the well-known adhesion proteins fibronectin and vitronectin, can function as an adhesion promoter on titanium materials.…”