Serum vitamin A and zinc levels of healthy people in northeast Thailand

Boonsiri, Patcharee; Pooart, Jureerut; Tangrassameeprasert, Roongpet; Hongsprabhas, Pranithi; Khampitak, Tueanjit; Yongvanit, Puangrat

doi:10.1016/j.cca.2006.05.020

Cited by 13 publications

(16 citation statements)

References 39 publications

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“…Reference values of carotenoids are not yet established for Cameroonian population. But our values were closely related to those obtained on healthy subjects elsewhere by previous workers (Boonsiri, Pooart, Tangrassameeprasert, & Hongsprabhas, 2007;Ribaya-Mercado, Maramag, Tengco, Blumberg, & Solon, 2008;Valtuena, Breidenassel, Folle, & Gonzá lez-Gross, 2011;Kiokias et al, 2016). Alpha-tocopherol values were lower than that of reference while other parameters fitted with their respective references (Gouado et al, 2005).…”

Section: Characteristics Of the Subjects Before Each Interventionsupporting

confidence: 71%

Bioavailability of Palm Oil Carotenoids Consumed from Different Foods

Dongho

Ndomou

Ngono

et al. 2017

JFR

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Crude palm oil (CPO), an ingredient with a high content in provitamins A, is usually consumed associated with foods that could affect carotenoids metabolism. This work aimed to evaluate the influence of foodstuffs prepared with the incorporation of CPO on carotenoids absorption. Thus, we studied carotenoids bioavailability in Cameroonian local meals based on CPO and three different foodstuffs: maize (Zea mais), cassava (Manihot esculente) or black eyed peas (Vigna unguiculata). Modified 'yellow sauce' prepared mainly with CPO was used as control meal. Eleven healthy volunteers with age range between 20-30 year and similar body mass index were submitted to the study. A total of four interventions (i.e., meal ingestion) were performed every six days. After meal ingestion blood samples were collected at 0, 2, 4 and 6 h, and carotenoids content were analyzed by HPLC. Results showed that control meal had the highest carotenoids absorption (0.479±0.063 µg.h/ml.), followed by maize (0.329±0.115 µg.h/ml) and cassava (0.141±0.075 µg.h/ml) cakes. Food based on black eyed peas incorporated with CPO showed the smallest increase in blood carotenoids (0.053±0.062 µg.h/ml). Notably, CPO consumption leads to a short term significant increase of blood carotenoids (p<0.05) that declines over time. Our results suggest that the association of CPO with different foodstuffs significantly affects carotenoids bioavailability. This effect seems to be more important for leguminous, followed by tubers and cereals.

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Section: Characteristics Of the Subjects Before Each Interventionsupporting

confidence: 71%

Bioavailability of Palm Oil Carotenoids Consumed from Different Foods

Dongho

Ndomou

Ngono

et al. 2017

JFR

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“…6,22,25 Many studies have indicated that it is necessary to use a gradient elution for the complete chromatographic separation of vitamins 1,3,11,15,19,20,22,25 and other works have described an isocratic elution. [4][5][6][12][13][14]16,17,21,23 Gradient elution involves a substantially longer chromatographic time, compared to the isocratic method, and also requires a rebalance of the HPLC system after injections. 12 So, methods that use isocratic elution are faster and less laborious.…”

Section: Introductionmentioning

confidence: 99%

“…6,[7][8][9] High-performance liquid chromatographic (HPLC) methods are considered the best choice for the determination of lipid-soluble vitamins. The first method developed for the quantification of these vitamins was described in 1973 10 and up to now several studies have reported new methods for the quantification of these compounds with the use of various mobile phases, 1,[3][4][5][6]11,[12][13][14][15] the adoption of chromatographic columns with either normal 15,16 or reverse phases, 3,4,6,11,[17][18][19][20][21] and also different detectors, such as Vis, UV (DAD), 1,[3][4][5][6]11,[12][13][14][15][17][18][19][20][22][23][24][25] fluorescence (FLD), 4,6,15,…”

Section: Introductionmentioning

confidence: 99%

“…Several procedures for sample extraction have been reported for vitamins, some of them based on protein precipitation using an organic solvent such as ethanol, methanol, isopropanol or acetonitrile, followed by liquid-liquid extraction and/or evaporation of the organic solvent under nitrogen stream and posterior reconstitution, 1,[3][4][5][11][12][13]16,17,[19][20][21][22][23][24]26 but these methods are normally very time-consuming. Extraction reagents include n-hexane, petroleum ether, dichloro methane-methanol, chloroform, ethyl acetate and n-butanol 1,[3][4][5][6][11][12][13]16,17,[19][20][21][22][23][24][25][26] with the addition of antioxidants such as butylated hydroxytoluene (BHT) 4,6,13,16,[19][20][21][22][23] or ascorbic acid 5 to avoid oxidation of lipidsoluble vitamins.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous quantification of lycopene, β-carotene, retinol and α -tocopherol in plasma after a simple extraction procedure: stability study and application to human volunteers

Charão¹,

Moro²,

Brucker³

et al. 2012

J. Braz. Chem. Soc.

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Um método para quantificação simultânea de licopeno, β-caroteno, retinol e α-tocoferol por cromatografia líquida de alta eficiência (HPLC) com detecção no visível/fluorescente e eluição isocrática foi otimizado e validado. O método consiste de extração líquido-líquido rápida e simples e posterior quantificação do sobrenadante extraído por HPLC. Alíquotas de plasma foram estocadas a -20°C por três meses para estudo da estabilidade. Aplicação metodológica foi realizada em amostras fornecidas por pintores e indivíduos não expostos a tintas (n = 75). O ensaio foi linear para todas as vitaminas analisadas (r > 0,99). Precisões intradia e interdia apresentaram coeficiente de variação (CV) menor que 5%. Exatidões variaram de 0,29 a -5,80% e recuperações entre 92,73 e 101,97%. Amostras de plasma e sobrenadante extraído foram estáveis por até 60 dias a -20°C. Foi demonstrada uma diminuição significativa nas concentrações de licopeno, β-caroteno e retinol em indivíduos expostos quando comparados com os não-expostos (p < 0,05). O método é simples, reprodutível, preciso, exato e sensível, e pode ser utilizado na rotina de laboratórios clínicos.A method for the simultaneous quantification of lycopene, β-carotene, retinol and α-tocopherol by high-performance liquid chromatography (HPLC) with Vis/fluorescence detection with isocratic elution was optimized and validated. The method consists of a rapid and simple liquid-liquid extraction procedure and a posterior quantification of extracted supernatants by HPLC. Aliquots of plasma were stored at -20°C for three months for stability study. The methodology was applied to samples from painters and individuals not exposed to paints (n = 75). The assay was linear for all vitamins (r > 0.99). Intra-and inter-run precisions were obtained with coefficient of variation smaller than 5%. The accuracies ranged from 0.29 to -5.80% and recoveries between 92.73 and 101.97%. Plasma samples and extracted supernatants were stable for 60 days at -20°C. A significant decrease of lycopene, β-carotene and retinol concentrations in plasma from exposed individuals compared to non-exposed individuals (p < 0.05) was observed. The method is simple, reproducible, precise, accurate and sensitive, and can be routinely utilized in clinical laboratories.

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“…Only 2 studies reported higher mean zinc concentrations in female than male cases (25,26). In other study, the prevalence of zinc deficiency was 31% (27).…”

Section: Discussionmentioning

confidence: 99%

Reference Range for Zinc Level in Young Healthy Population in Southeast of Iran

et al. 2016

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Background: There are different controversial reports about Zinc status in healthy and normal population. The aim of this study is to evaluate Zinc status in normal and healthy population. Methods: This cross-sectional study conducted on 320 subjects aged between 10 -30 years without any underlying diseases or history of mineral therapy in Zahedan, south east of Iran. Zinc level was measured in all subjects after 12 hours fasting. All blood samples were centrifuged following 45 minutes spontaneous coagulation and then stored at -20°C. Zinc level was measured using graphite furnace atomic absorption spectrometry, Varian, Australia (spectr AA 240fs, 2009, USA).

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Serum vitamin A and zinc levels of healthy people in northeast Thailand

Cited by 13 publications

References 39 publications

Bioavailability of Palm Oil Carotenoids Consumed from Different Foods

Bioavailability of Palm Oil Carotenoids Consumed from Different Foods

Simultaneous quantification of lycopene, β-carotene, retinol and α -tocopherol in plasma after a simple extraction procedure: stability study and application to human volunteers

Reference Range for Zinc Level in Young Healthy Population in Southeast of Iran

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