Serum tsRNA as a novel molecular diagnostic biomarker for lupus nephritis

Zhang, Xiaoshan; Yang, Ping; Khan, Adeel; Xu, Dongjie; Chen, Shanshan; Zhai, Junbin; Zhang, Bin; Xiong, Tao; Li, Han; Li, Zhiyang

doi:10.1002/ctm2.830

Cited by 3 publications

(3 citation statements)

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“…More importantly, the content of tsRNAs in vesicles were significantly higher than miRNAs (39)(40)(41). tsRNAs in serum as biomarkers of SLE and LN patients were confirmed in our previous studies (14,15).Based on the perspective of non-invasive liquid biopsy, this was the first report to explore whether urinary exosome derived tsRNA signatures could be considered as biomarkers for distinguishing LN from SLE patients…”

Section: Discussionsupporting

confidence: 73%

“…Furthermore, tsRNAs exert an essential role in the pathophysiology of biological processes by connecting with proteins or mRNA, regulating gene expression, and inhibiting translation (13). Mounting evidence has indicated that the aberrated expression of tsRNAs are deeply implicated in cancer, parasitic disease (12)(13)(14)(15)(16). Recently, a few studies including our previous research pay attention to the relationship between SLE and tsRNA (14,(17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

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Urinary exosome tsRNAs as novel markers for diagnosis and prediction of lupus nephritis

et al. 2023

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ObjectiveLupus nephritis (LN) is one of the most severe organ manifestations of systemic lupus erythematosus (SLE). Early identification of renal disease in SLE is important. Renal biopsy is currently recognized as the gold standard for diagnosing LN, however, it is invasive and inconvenient for dynamic monitoring. Urine has been considered more promising and valuable than blood in identifying inflamed kidney tissue. Here, we determine whether the signatures of tRNA-derived small noncoding RNA (tsRNA) in urinary exosomes can serve as novel biomarkers for the diagnosis of LN.MethodstsRNA sequencing was performed in exosome extracted from pooled urine of 20 LN patients and 20 SLE without LN, and the top 10 upregulated tsRNAs were screened as candidate markers of LN. The candidate urinary exosomal tsRNAs were primarily elected by TaqMan probe-based quantitative reverse transcription-PCR (RT-PCR) in 40 samples (20 LN and 20 SLE without LN) in the training phase. In the validation phase, selected tsRNAs from the training phase were further confirmed in a larger cohort (54 LN patients and 39 SLE without LN). Receiver operating characteristic curve (ROC) analysis was conducted to evaluate the diagnostic efficacy.ResultsUpregulated levels of tRF3-Ile-AAT-1 and tiRNA5-Lys-CTT-1 in the urinary exosomes were observed in LN compared with SLE without LN (P < 0.0001 and P < 0.001) and healthy controls (P < 0.01 and P < 0.01), with the area under the curve (AUC) of 0.777 (95% CI: 0.681-0.874, sensitivity 79.63%, specificity 66.69%) and 0.715 (95% CI: 0.610-0.820, sensitivity 66.96%, specificity 76.92%) for discriminating LN from SLE without LN patients. SLE patients with mild activity and moderate to severe activity had higher levels of urinary exosome derived tRF3-Ile AAT-1 (P = 0.035 and P < 0.001) and tiRNA5-Lys-CTT-1 (P = 0.021 and P < 0.001) compared with patients with no activity. Moreover, bioinformatics analysis revealed that both of the tsRNAs regulate the immune process by modulating metabolism and signal pathway.ConclusionIn this study, we demonstrated that urinary exosome tsRNAs can be served as noninvasive biomarkers for the efficient diagnosis and prediction of nephritis in SLE.

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Section: Discussionsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Urinary exosome tsRNAs as novel markers for diagnosis and prediction of lupus nephritis

et al. 2023

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“…Ping Yang 1,2 Yifan Sun 1,3 Chenlan Wang 2 Zhibo Li 4 Yiyuan Han 4 Jianming Gong 1 Adeel Khan 5 Jin Wang 2 Yanbo Wang 2 Fangfang Jin 4 Zhiyang Li 1,3…”

Section: A U T H O R C O N T R I B U T I O N Smentioning

confidence: 99%

Serum exosomal tsRNA biomarkers: A novel strategy for identifying lupus nephritis

Yang,

Sun,

Wang

et al. 2024

Clinical & Translational Med

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Dear Editor,For effective treatment and control of systemic lupus erythematosus (SLE), it is pertinent to be able to precisely identify and predict lupus nephritis (LN). 1 The current gold standard for this is renal biopsy, but this is invasive, making it less attractive for dynamic monitoring of disease progression. 2,3 tRNA-derived small non-coding RNAs (tsRNAs), as a novel RNA biomarker, intricately involved in regulating various stages of gene expression, from TA B L E 1 Clinical and immunological characteristics of the systemic lupus erythematosus (SLE) participants. Training Verification Index SLE (LN-) LN SLE (LN-) LN HC Cases 17 23 105 108 80 Gender (male/female) 1/16 1/22 7/98 5/103 17/63

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Biological function and clinical application prospect of tsRNAs in digestive system biology and pathology

Du,

Huang,

Zheng

et al. 2023

Cell Commun Signal

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AbstracttsRNAs are small non-coding RNAs originating from tRNA that play important roles in a variety of physiological activities such as RNA silencing, ribosome biogenesis, retrotransposition, and epigenetic inheritance, as well as involvement in cellular differentiation, proliferation, and apoptosis. tsRNA-related abnormalities have a significant influence on the onset, development, and progression of numerous human diseases, including malignant tumors through affecting the cell cycle and specific signaling molecules. This review introduced origins together with tsRNAs classification, providing a summary for regulatory mechanism and physiological function while dysfunctional effect of tsRNAs in digestive system diseases, focusing on the clinical prospects of tsRNAs for diagnostic and prognostic biomarkers.

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Serum tsRNA as a novel molecular diagnostic biomarker for lupus nephritis

Cited by 3 publications

References 10 publications

Urinary exosome tsRNAs as novel markers for diagnosis and prediction of lupus nephritis

Urinary exosome tsRNAs as novel markers for diagnosis and prediction of lupus nephritis

Serum exosomal tsRNA biomarkers: A novel strategy for identifying lupus nephritis

Biological function and clinical application prospect of tsRNAs in digestive system biology and pathology

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