Serum ribonucleases in cancer: Relation to tumor histology

Chretien, Paul B.; Matthews, Wilbert; Twomey, Patrick

doi:10.1002/1097-0142(197301)31:1<175::aid-cncr2820310124>3.0.co;2-c

Cited by 34 publications

(10 citation statements)

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“…The results of the present study agree with those of other investigators who have reported increases in the level of serum RNase in cancer patients (Zytko and Cantero, 1962;Chretien et al, 1973;Catalona et al, 1973;Drake et al, 1975;Reddi and Holland, 1976;Sheid et al, 1977). For the samples from Scripps Clinic, the percentage of abnormal sera in individual cancers ranged from 62% for leukaemias to 89% for Hodgkin's disease.…”

Section: Rlnase Assaysupporting

confidence: 93%

“…Zytko and Cantero (1962) reported that 600% of patients with malignant diseases demonstrated serum RNase levels that were significantly higher than those of normal individuals. Chretien et al (1973) suggested a relationship between serum RNase levels and tumour histology, finding that sera from individuals with adenocarcinomas and squamous carcinomas yielded elevated activities, while those from sera of patients with sarcomas and melanomas were not significantly different from normal values. Catalona et al (1973) observed increased RNase levels in a majority of the sera from patients with various urological cancers.…”

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confidence: 99%

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Serum ribonuclease activity in cancer patients

Kottel¹,

Hoch²,

Parsons³

et al. 1978

Br J Cancer

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Section: Rlnase Assaysupporting

confidence: 93%

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confidence: 99%

Serum ribonuclease activity in cancer patients

Kottel¹,

Hoch²,

Parsons³

et al. 1978

Br J Cancer

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“…In each study, control subjects were apparently normal and did not include patients with nonmalignant diseases. Correlation of the degree of increase of RNase activity with the extent of neoplasia or thera- peutic response was variable; five studies (8-10, 12, 14) found a correlation and two (11,12) (19), and allowed use of a higher pH, emphasizing possible contributions of pancreatic RNase with its higher pH optimum (20) to serum activity. Serum RNase activity is not "absolutely dependent" on phosphate or citrate as stated by Reddi (20).…”

Section: Discussionmentioning

confidence: 99%

Serum RNase in the diagnosis of pancreatic carcinoma.

Peterson¹

1979

Proc. Natl. Acad. Sci. U.S.A.

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Serum RNase (RNase I; ribonucleate 3'-pyrimidino-oligonucleotidohydrolase, EC 3.1.4.22) activity (mean ± SD) with polycytidine as substrate was determined in normal individuals (24.9 ± 3.0 units/ml) and in patients with pancreatic cancer (37.3 + 14.8), pancreatitis (38.5 ± 12.6), nonpancreatic diseases (48.7 ± 14.8), or renal failure (175.8 : 92.8 Blood drawn from patients and controls was allowed to clot and then was centrifuged; serum was removed within 2 hr of collection. The serum was stored at -20'C. Freezing, thawing, and frozen storage for at least 1 month do not affect RNase activity.The assay for serum RNase activity was based on the method for bovine pancreatic RNase first reported by Zimmerman and Sandeen (2) with the synthetic substrate polycytidine. Serum was diluted 1:1000 with 5 mM Tris at pH 7 containing 0.01% bovine serum albumin, and a 50-sl aliquot was added to a Microfuge tube containing 150 Al of 0.1 M Tris at pH 8 and 50 /1l of polycytidine at 1 mg/ml. After incubation for 20 min at 370C, 250-A.l of cold 1.2 M perchloric acid/20 mM La203 was added. After 10 min at 00C, the mixture was centrifuged at 8000 X g for 5 min at room temperature. The supernatant, containing acid-soluble nucleotide, was diluted with water and the A280 was measured in a Beckman spectrophotometer. All serum dilutions and assays were performed in duplicate. Substrate blanks containing all reagents but no enzyme (serum) were analyzed in an identical manner and the values were subtracted from each assay. An enzyme (serum) blank proved unnecessary because serum did not contribute to the absorbance at these low dilutions. Sera assayed previously or bovine pancreatic RNase served as standards and their analysis was repeated with each group of sera of unknown activity. One RNase unit is the amount of enzyme activity that produces 1 ,mol of acid-soluble nucleotide per min at 37°C in 0.06 M Tris at pH 8. Molarity of acid-soluble nucleotide was calculated on the basis of an extinction coefficient of 13.0 X 103 at pH 2.0 (3).In 17 determinations on aliquots of one serum sample, performed consecutively, the mean (±SD) was 22.4 ± 0.7 units/ml. In 6 to 17 determinations on sera of four normal individuals obtained and performed at three different times, the mean was 21.3 ± 0.8 units/ml. Overall, 117 determinations were performed on the three samples of these four individuals; the results demonstrate remarkable precision of the procedure. As would be desired, the methodological variation is significantly less than the biologic variability: the mean of analyses on sera from 20 female and 20 male normal individuals was 24.9 + 3.0 units/ ml.The activity of bovine pancreatic RNase was the same when assayed with the polycytidine substrate used in my laboratory or with that used by Zimmerman and Sandeen (2) (Fig. 1A).When increasing amounts of three different sera were added to substrate, the rate of appearance of reaction products was linear (Fig. 1B).Patients were divided into five groups according to the following criteria. 263...

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“…The activities of various other enzymes involved in the biosynthesis of pyrimidines and nucleic acids also increase in malignancy (Ferdinandus et al 1971). Investigations into other possible serum markers have indicated increases of several enzymes involved in nucleic acid degradation, particularly increases in alkaline deoxyribonucleases in patients with oral cancer (Scully et al 1982) and ribonucleases in patients with head and neck cancer (Chretien et al 1973). Such enzymes may provide the nucleoside substrates for TdR kinase activity.…”

Section: Discussionmentioning

confidence: 99%