Serum Proteins by Capillary Zone Electrophoresis: Approaches to the Definition of Reference Values

Petrini, Concetta; Alessio, Maria Grazia; Scapellato, Luisa; Brambilla, Simona; Franzini, Carlo

doi:10.1515/cclm.2000.144

Cited by 5 publications

(6 citation statements)

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“…All these data were found to compare well with those reported by Ihara et al [170]. Petrini et al [171] addressed the definition of reference values, pointing out the importance for a correct definition of the population and statistical evaluation from which reference values are derived. The data of 167 serum samples from individuals that were classified as normal by high-resolution cellulose acetate electrophoresis were employed to define the reference values for the five serum protein fractions.…”

Section: General Screening For Serum Proteinssupporting

confidence: 77%

Advances of capillary electrophoresis in clinical and forensic analysis (1999-2000)

et al. 2001

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In this paper, capillary electrophoresis in clinical and forensic analysis is reviewed on the basis of the literature of 1999, 2000 and the first papers in 2001. An overview of progress relevant examples for each major field of application, namely (i) analysis of drug seizures, explosives residues, gunshot residues and inks, (ii) monitoring of drugs, endogenous small molecules and ions in biofluids and tissues, (iii) general screening for serum proteins and analysis of specific proteins (carbohydrate deficient transferrin, alpha1-antitrypsin, lipoproteins and hemoglobins) in biological fluids, and (iv) analysis of nucleic acids and oligonucleotides in biological samples, including oligonucleotide therapeutics, are presented.

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Section: General Screening For Serum Proteinssupporting

confidence: 77%

Advances of capillary electrophoresis in clinical and forensic analysis (1999-2000)

et al. 2001

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“…Despite good correlation with the Hydrasys-Hyrys for albumin and ␥-globulins (r ϭ 0.99), there was an ϳ200% positive difference vs AGE for ␣ 1 -globulins and a 15% negative difference for albumin. Reports in the literature indicate similar results for the Paragon CZE2000 (ϳ100% difference for ␣ 1 -globulins, but lower difference for albumin) (8,9 ) and the Capillarys (14 ), highlighting the need for an adjustment of the protein fraction reference interval (9,18 ). Comparisons with immunonephelometry and colorimetry suggest that quantification of albumin and ␣ 1 -globulin in the low-UV range is more accurate than protein staining (8,9 ), especially for ␣ 1 -acid glycoprotein because of its high sialic acid content.…”

Section: Discussionmentioning

confidence: 55%

Automated Multicapillary Electrophoresis for Analysis of Human Serum Proteins

et al. 2003

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Background:We evaluated a new, automated multicapillary zone electrophoresis (CE) instrument (Capillarys ® , 4.51 software version; Sebia) for human serum protein analysis. Methods: With the Capillarys ␤1-␤2؉® reagent set, proteins were separated at 7 kV for 4 min in 15.5 cm ؋ 25 m fused-silica capillaries (n ‫؍‬ 8) at 35.5°C in a pH 10 buffer with online detection at 200 nm. Serum samples with different electrophoretic patterns (n ‫؍‬ 265) or potential interference (n ‫؍‬ 69) were analyzed and compared with agarose gel electrophoresis (AGE; Hydrasys ® -Hyrys ® , Hydragel protein(e) 15/30 ® reagent set; Sebia). Results: CVs were <3.5% for albumin, <11% for ␣ 1 -globulin, <4.1% for ␣ 2 -globulin, <7.4% for ␤-globulin, and <5.8% for ␥-globulin (3 control levels); measured throughput was 60 samples/h. In patients without paraprotein (n ‫؍‬ 116), the median differences between CE and AGE were ؊5.4 g/L for albumin, 4.0 g/L for ␣ 1 -globulin, 0.7 g/L for ␣ 2 -globulin, 0.6 g/L for ␤-globulin (P <0.001 for all fractions), and ؊0.1 g/L for ␥-globulin (not significant). More samples had at least one ␥-migrating peak detected by CE (n ‫؍‬ 135 vs 130; paraprotein detection limit, ϳ0.5-0.7 g/L), but fewer were quantified (n ‫؍‬ 84 vs 91) because of ␥-to ␤-migration shifts. There was a 1.2 g/L median difference between CE and AGE for ␥-migrating paraprotein quantification (n ‫؍‬ 69; P <0.001). Several ultraviolet-absorbing substances (lipid

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“…However, by appropriate adjustment of the normal ranges to account for these differences, the clinical interpretation will not be affected. To better determine the normal range expected for CE, Petrini et al [46] ran a small number of samples (N = 167) that were identified as normal by CAE. Using the results from this small study they then validated the range using 1000 normal and abnormal serum samples.…”

Section: Serum Proteinsmentioning

confidence: 99%

Capillary electrophoresis and the clinical laboratory

et al. 2006

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Over the past 15 years, CE as an analytical tool has shown great promise in replacing many conventional clinical laboratory methods, such as electrophoresis and HPLC. CE's appeal was that it was fast, used very small amounts of sample and reagents, was extremely versatile, and was able to separate large and small analytes, whether neutral or charged. Because of this versatility, numerous methods have been developed for analytes that are of clinical interest. Other than molecular diagnostic and forensic laboratories CE has not been able to make a major impact in the United States. In contrast, in Europe and Japan an increasing number of clinical laboratories are using CE. Now that automated multicapillary instruments are commercially available along with cost-effective test kits, CE may yet be accepted as an instrument that will be routinely used in the clinical laboratories. This review will focus on areas where CE has the potential to have the greatest impact on the clinical laboratory. These include analyses of proteins found in serum and urine, hemoglobin (A1c and variants), carbohydrate-deficient transferrin, forensic and therapeutic drug screening, and molecular diagnostics.

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Serum Proteins by Capillary Zone Electrophoresis: Approaches to the Definition of Reference Values

Cited by 5 publications

References 0 publications

Advances of capillary electrophoresis in clinical and forensic analysis (1999-2000)

Advances of capillary electrophoresis in clinical and forensic analysis (1999-2000)

Automated Multicapillary Electrophoresis for Analysis of Human Serum Proteins

Capillary electrophoresis and the clinical laboratory

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