Serum Protein Profiling of Adults and Children With Crohn Disease

Abstract

Objectives Crohn’s disease (CD) and ulcerative colitis (UC), known collectively as inflammatory bowel disease (IBD), are chronic immuno-inflammatory pathologies of unknown etiology. Despite the frequent utilization of biomarkers in medical practice, there is a relative lack of information regarding validated paediatric biomarkers for IBD. Further, biomarkers proved to be efficacious in adults are frequently extrapolated to the paediatric clinical setting without considering that the pathogenesis of many diseases is distinctly different in children. In the current study, proteomics technology was employed in order to monitor differences in protein expression among adult and children CD patients, in order to identify a panel of candidate protein biomarkers that might be used to improve prognostic-diagnostic accuracy and to advance paediatric medical care. Methods Male and female serum samples from 12 adults and 12 children with active CD were subjected to two-dimensional gel electrophoresis. Following the relative quantitation of protein spots exhibiting a differential expression between the two groups by densitometry, the spots were further characterized by MALDI-TOF-MS. Results were confirmed by Western blot analysis. Results Clusterin (CLUS) was found to be significantly over-expressed in adults with CD, whereas ceruloplasmin (CERU) and apolipoprotein B-100 (APOB) were found to be significantly over-expressed in children indicating that the expression of these proteins might be implicated in the onset or progression of CD in these two sub-groups of patients. Conclusions Interestingly, we found a differential expression of several proteins in adults versus paediatric CD patients. Undoubtedly, future experiments using a larger cohort of CD patients are needed to evaluate the relevance of our preliminary findings.

Urine Proteome Changes in a TNBS‐Induced Colitis Rat Model

“…They also found a differential expression of several proteins in adults versus pediatric CD patients. CLUS was found to be significantly overexpressed in adults with CD, whereas CERU and APOB were found to be significantly overexpressed in children . Compared to their results, the differential proteins we reported were not coincident with none of them.…”

“…Tsangaris et al. focused on identifying serum protein biomarkers of CD using 2D gel electrophoresis . They report on 15 proteins differentially present in the serum of CD patients following IFX treatment.…”

Current application of proteomics in biomarker discovery for inflammatory bowel disease

“…Similar studies using variants of mass spectrometry have yielded similar results where protein profiles could accurately distinguish between selected UC and CD cases [2427] . A recent study by Vaiopoulou et al [28] sought to investigate pediatric biomarkers for CD by comparing the proteomic profile between adult and pediatric CD patients. 3 proteins (ceruloplasmin, clusterin and apolipoprotein B100) were shown to be significantly different between the two cohorts.…”

“…Label free method developed could accurately distinguish synthetic spiked samples of serum CD: 15 SELDI-TOF Sumramanian et al [26] Serum CD: 48 Protein signature of 12 mass: Charge peaks could classify CD with approximately equal 95% sensitivity/specificity UC: 62 4 proteins identified as clinically useful Nanni et al [27] Serum [28] Serum CD: 24 (12 adults, 12 children)…”

Laboratory predictors of gastrointestinal tract diseases

“…В ис-следовании A. Vaiopoulou и соавт. у таких пациентов выявлена повышенная экспрессия белка кластерина (участвующего в иммунных реакциях), медьсодержа-щего гликопротеина церулоплазмина, а также аполи-протеина B-100 (отвечающего за синтез липопроте-инов очень низкой плотности) [4]. Изученные белки могут потенциально использоваться в качестве пре-дикторов рецидива и прогрессирования воспалитель-ных болезней кишечника.…”