Serum protein profiling in diffuse large B‐cell lymphoma

Riby, Jacques; Mobley, James A.; Bracci, Paige M.; Skibola, Christine F.

doi:10.1002/prca.201600074

Cited by 8 publications

(6 citation statements)

References 41 publications

(41 reference statements)

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“…ECM1 can regulate M1 macrophage polarization through the granulocyte-macrophage colony-stimulating factor/ STAT5 signaling pathway in in ammatory bowel disease (IBD) 16 . It has also been reported that ECM1 was statistically signi cantly elevated in diffuse large B-cell lymphoma patient sera as compared to controls 17 . Here we report that ECM1 is also involved in B-ALL.…”

Section: Discussionmentioning

confidence: 82%

High Expression of ECM1 At Diagnosis Predicated Poor Prognosis in Adult B-Cell Acute Lymphoblastic Leukemia

Wang

et al. 2021

Preprint

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Identifying new prognostic biomarkers and selecting treatment regimens based on them can improve outcomes of adult patients with B-cell acute lymphoblastic leukemia (B-ALL). Extracellular matrix protein 1(ECM1) is a glycoprotein involved in many biologic processes. Here we aimed to determine the expression and the prognostic value of ECM1 in B-ALL. One hundred and six newly-diagnosed B-ALL patients (seventy-one adult patients and thirty-five children patients) were enrolled from March 2013 to December 2018. The ECM1 transcript levels were significantly different among different disease state. Among 71 adult B-ALL patients, ECM1-high group has a higher initial platelet count( p=0.004)，more BCR-ABL fusion positive patients (p=0.004) and poor risk patients (p=0.021) than ECM1-low group, whereas no significant relationship with any other clinical characteristics was observed. ECM1-high group had significantly lower 3-year OS (11.4% vs. 36.1%, p=0.015) and 3-year RFS (9.7% vs. 29.4%, p=0.047) compared to ECM1-low group. In the multivariate Cox regression model, high ECM1 expression was an independent adverse prognostic factor for OS(HR=3.0(1.4-6.6), p=0.007). Our data demonstrated that the expression of ECM1 could serve as a novel prognostic biomarker for adult patients with B-ALL.

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Section: Discussionmentioning

confidence: 82%

High Expression of ECM1 At Diagnosis Predicated Poor Prognosis in Adult B-Cell Acute Lymphoblastic Leukemia

Wang

et al. 2021

Preprint

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“…The mass spectrometric analysis of hippocampal proteins was done as previously described 31 . Briefly, using a split flow configuration, peptide digests were injected into a dSurveyor HPLC plus (Thermo Fisher Scientific, MA, USA) and data were collected in CID mode.…”

Section: Methodsmentioning

confidence: 99%

Supraphysiological Levels of Oxygen Exposure During the Neonatal Period Impairs Signaling Pathways Required for Learning and Memory

Ramani

Kumar

Halloran

et al. 2018

Sci Rep

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Preterm infants often require prolonged oxygen supplementation and are at high risk of neurodevelopmental impairment. We recently reported that adult mice exposed to neonatal hyperoxia (postnatal day [P] 2 to 14) had spatial navigation memory deficits associated with hippocampal shrinkage. The mechanisms by which early oxidative stress impair neurodevelopment are not known. Our objective was to identify early hyperoxia-induced alterations in hippocampal receptors and signaling pathways necessary for memory formation. We evaluated C57BL/6 mouse pups at P14, exposed to either 85% oxygen or air from P2 to 14. We performed targeted analysis of hippocampal ligand-gated ion channels and proteins necessary for memory formation, and global bioinformatic analysis of differentially expressed hippocampal genes and proteins. Hyperoxia decreased hippocampal mGLU7, TrkB, AKT, ERK2, mTORC1, RPS6, and EIF4E and increased α3, α5, and ɤ2 subunits of GABAA receptor and PTEN proteins, although changes in gene expression were not always concordant. Bioinformatic analysis indicated dysfunction in mitochondria and global protein synthesis and translational processes. In conclusion, supraphysiological oxygen exposure reduced proteins necessary for hippocampus-dependent memory formation and may adversely impact hippocampal mitochondrial function and global protein synthesis. These early hippocampal changes may account for memory deficits seen in preterm survivors following prolonged oxygen supplementation.

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“…The detailed procedure of host cell protein extraction and digestion was described in previous publications [18, 19]. In brief, the host cell proteins were first extracted from cell pellets using M-PER, denatured and run into a 10% SDS Bis-Tris PAGE.…”

Section: Methodsmentioning

confidence: 99%

“…LC-MS/MS was applied to acquire the high-quality peptide precursor and fragment ion data as described in literature [19]. Each proteomics sample was injected to LC-MS/MS with triplication.…”

Section: Methodsmentioning

confidence: 99%

Comparative proteomic analysis of three Chinese hamster ovary (CHO) host cells

Sun

et al. 2017

Biochemical Engineering Journal

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Chinese hamster ovary (CHO)1 cells have been widely used to express heterologous genes and produce therapeutic proteins in biopharmaceutical industry. Different CHO host cells have distinct cell growth rates and protein expression characteristics. In this study, the expression of about 1,307 host proteins in three sublines, i.e. CHO K1, CHO S and CHO/dihydrofolate reductase (dhfr)−, were investigated and compared using proteomic analysis. The proteins involved in cell growth, glycolysis, tricarboxylic acid cycle, transcription, translation and glycosylation were quantitated using Liquid chromatography tandem-mass spectrometry (LC-MS/MS). The key host cell proteins that regulate the kinetics of cell growth and the magnitude of protein expression levels were identified. Furthermore, several rational cell engineering strategies on how to combine the desired features of fast cell growth and efficient production of therapeutic proteins into one new super CHO host cell have been proposed.

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Serum protein profiling in diffuse large B‐cell lymphoma

Abstract: These preliminary data when combined with other cancer-related data regarding these proteins warrant continued research in clinical and large prospective studies to clarify the role for these biomarkers in DLBCL pathogenesis and/or prognosis.

Cited by 8 publications

References 41 publications

High Expression of ECM1 At Diagnosis Predicated Poor Prognosis in Adult B-Cell Acute Lymphoblastic Leukemia

High Expression of ECM1 At Diagnosis Predicated Poor Prognosis in Adult B-Cell Acute Lymphoblastic Leukemia

Supraphysiological Levels of Oxygen Exposure During the Neonatal Period Impairs Signaling Pathways Required for Learning and Memory

Comparative proteomic analysis of three Chinese hamster ovary (CHO) host cells

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