Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite

Abstract

The objective of our study was to determine the effects of clinoptilolite supplemented in colostrum on the blood serum protein electrophoretic pattern of new-born calves. Methods: Romanian Black and White new-born calves involved in the study were divided into 3 groups: the control group (C) that received colostrum without clinoptilolite, and experimental groups I (E1) and II (E2) that received colostrum supplemented with 0.5% and 2% clinoptilolite, respectively. The concentration of total protein and protein fractions (albumin, α1-globulin, α2-globulin, β-globulin and γ-globulin) were analyzed by electrophoresis on cellulose acetate. Results: At hour 30 after birth, concentrations of γ-globulins, β-globulin and total protein in E1 group of calves were higher than in control group by 42.11% (p < 0.05), 28.48% (p > 0.05) and 18.52% (p > 0.05), respectively, and were higher, but not significantly, in group E2 compared to the control group. This was in accordance with a significant lower albumin/globulin ratio in groups E1 and E2 (29.35%, p < 0.05 and 35.87%, p < 0.05, respectively) than in control group at 30 h postpartum, which indicates an obvious increase of the globulins fraction in experimental groups. The conclusion: Clinoptilolite was effective in improving passive transfer in new-born calves, but it was more effective if added in colostrum with a dose of 0.5% than with a dose of 2%.

Selenium and Natural Zeolite Clinoptilolite Supplementation Increases Antioxidative Status and Immune Response in Growing Pigs

“…The microvilli length was higher and the number of microvilli per square µm was higher (37), which could point to a better absorption capability. The other experiment with the newborn calves has demonstrated a better passive transfer of immunoglobulin into newborns (38).…”

An assessment of serum‐dependent impacts on intracellular accumulation and genomic response of per‐ and polyfluoroalkyl substances in a placental trophoblast model

“…Human serum contains approximately 60‐80 mg/mL proteins, of which about 50‐60% are albumins, and it is reported that PFOA and PFOS bind >99% of available human or bovine serum albumin 34,35 . This affinity for albumin which is present in the FBS at ~4.5 mg/mL protein and ~ 43 mg/mL protein in newborn calf serum 36 would sequester PFOS and PFOA in the media, effectively reducing intracellular availability of PFOA and PFOS in SSM treatments. While both higher than HFPO‐DA, PFOS and PFOA also displayed different trends in internal accumulation by dose.…”

“…24,25,34 Human serum contains approximately 60-80 mg/mL proteins, of which about 50-60% are albumins, and it is reported that PFOA and PFOS bind >99% of available human or bovine serum albumin. 34,35 This affinity for albumin which is present in the FBS at 4.5 mg/mL protein and 43 mg/mL protein in newborn calf serum 36 which regulates trophoblast proliferation as well as angiogenesis, has been shown in both maternal serum and placenta to be inversely associated with preeclampsia. 43,44 In addition, pro-apoptotic markers BAD and BAX were up-regulated and anti-apoptotic BCL2 was downregulated in both SFM and SSM after exposure to PFOA, PFOS, or HFPO-DA.…”

Zeolites Applications in Veterinary Medicine

“…Zeolites Applications in Veterinary Medicine DOI: http://dx.doi.org/10.5772/intechopen.87969 and 35.87%, p < 0.05, respectively) was reported, compared with the control group at 30 h postpartum, which indicates an obvious increase in globulin fractions in experimental groups. Clinoptilolite was effective in improving passive transfer in newborn calves, better if added in colostrum in a dose of 0.5% than in a dose of 2% (Figure 2) [67].…”