Serum Protein Changes after Injury

Abstract

S U M M A R Y1. By means of a quantitative immunoelectrophoretic technique, serum protein changes have been followed in seven patients before and during the week following inguinal herniorrhaphy, and in a single case of influenza.2. The twenty proteins studied are grouped according to whether they rise (orosomucoid, a,-antitrypsin, Gc-globulin, caeruloplasmin, haptoglobin, globulin and proteins numbered 45,99, 101 and X); fall (pre-albumin, a,,,-glycopro-teinandproteinNo. 9); or show no significant change (a,-macroglobulin, haemopexin). a,-Lipoprotein fell in the case of influenza but did not change in the hernia patients; a1 -easily-precipitable-glycoprotein increased in the hernia cases only.3. The nature of the 'acute phase reaction' and the factors which elicit it are discussed.Every serious study of plasma protein changes in pathological states is complicated by the unspecific response, commonly called 'the acute phase reaction', which occurs during any idammatory process. Although this is frequently quoted, there are only a few reliable accounts of the changes affecting individual plasma proteins after injury (Schumacher, 1962; Crockson, Payne, Ratcliff & Soothill, 1966;Werner & Odenthal, 1967; Laurell, personal communication). In this study we have followed the concentration of twenty different plasma proteins in time across a single surgical traumatic event. These include the serum proteins present in high concentration (over 100 mg/100 ml) but exclude the immunoglobulins and some proteins present in lower concentrations. It is appreciated that the selected proteins are not necessarily the only ones involved in the unspecific response; further work will be required to study the changes in other serum proteins.

Lowering of serum 3,3‘,5‐triiodothyronine thyroxine ratio in patients with myocardial infarction; relationship with extent of tissue injury

“…This is in contrast with the finding reported by Clarke [19], who showed a definite decrease of TBPA. Decreased TBPA and albumin associated with increased concentrations of the globulins is known as the acute phase reaction [20,21]. Serum T3 is reported to decrease in patients suffering from non-thyroidal diseases.…”

Sequential Changes of Plasma Proteins after Surgical Trauma

“…Werner and Cohen (28) have analyzed 10 proteins after A rather uniform pattern was obtained with Werners protein battery applied at an interval of three days between consecutive blood samplings. As judged from the course of plasma protein changes observed by Clarke et al who used a broader protein spectrum on a more homogeneous material, the inflammatory response may be the result of multiple stimuli (6).…”

Adaptive response due to changes in gene regulation: a study with Drosophila.

“…Antiserum was used to determine the amounts of ADH protein by antigenantibody immunoelectrophoresis (29). The procedure was modified from that in ref 30. Crude extract (1 il) was inserted in wells 1.5 mm in diameter.…”