Serum PON1 arylesterase activity in relation to hyperhomocysteinaemia and oxidative stress in young adult central retinal venous occlusion patients

Abstract

Aim To estimate the arylesterase activity of serum paraoxonase-1 (PON1-ARE), which is reported to have an antioxidant and antiatherogenic potential and to correlate with plasma homocysteine (Hcys) and plasma TBARS in young adult central retinal venous occlusion (CRVO) patients. Methods A case-control prospective study carried out in 10 CRVO patients (mean age 27 ± 5 years; 7 males, 3 females) and 20 healthy controls (mean age 29 ± 5 years; 15 males, 5 females). Results The CRVO patients showed a significantly lowered serum PON1-ARE activity (P ¼ 0.009) along with a significant increase in the levels of plasma Hcys (P ¼ 0.018) when compared to the control subjects. There was a negative correlation between serum PON1-ARE and plasma Hcys levels (P ¼ 0.058) as well as between PON1-ARE and plasma TBARS levels (P ¼ 0.001) in the CRVO patients. Conclusion This is the first report of lowered serum PON1-ARE level as a risk factor for CRVO (OR ¼ 1.108, CI ¼ 0.914, 1.314; P ¼ 0.296), which is found to correlate with oxidative stress.

Homocysteine induces oxidative stress in young adult central retinal vein occlusion

“…In our earlier study we had shown increased oxidative stress in CRVO cases as seen by elevated TBARS levels, a marker of lipid peroxidation, as well as decreased antioxidant activity of paraoxonase 33. However, there are no studies on the homocysteine-induced oxidative stress in CRVO cases.…”

Evaluation of oxidative stress balance in acute deep vein thrombosis

“…Some authors suggest that decreased antioxidant enzymes are associated with retinal vein and central vein thrombosis and that increased oxidative stress may predispose to venous occlusive disorders or venous thrombosis [29][30][31]. Our study suggests that thrombosis in deep veins of the lower extremity (DVT) may be associated with oxidative imbalance.…”

Retinal Vein Occlusion Induced by a MEK Inhibitor - Impact of Oxidative Stress on the Blood-Retinal Barrier

“…Thrombophilic risk factors related to RVO include hyperhomocysteinemia, methylenetetrahydrofolate reductase (MTHFR) gene mutation, factor V Leiden mutation, protein C and S deficiency, antithrombin deficiency, prothrombin gene mutation, anticardiolipin antibodies and lupus anticoagulant. High levels of circulating homocysteine may damage the vascular endothelium by releasing free radicals, creating a hypercoagulable environment (Angayarkanni et al, 2008). It appears that there is an association between RVO and hyperhomocysteinaemia and anti-phospholipid antibodies.…”

“…Many of the risk factors for RVO described above, such as alterations in blood flow, systemic vascular disorders, hypercoagulability, and elevated levels of pro-inflammatory cytokines, may also be associated with a state of oxidative stress (Simoncini et al, 2005). Indeed, in a case-control prospective study in young adult CRVO patients, serum levels of paraoxonase-1 arylesterase (PON1-ARE) activity, reported to have antioxidant potential, were found to be negatively correlated with hyperhomocysteinemia and lipid peroxidation, an indicator of oxidative stress (Angayarkanni et al, 2008). Decreased levels of PON1-ARE activity as well as increased levels of the lipid peroxidation marker were shown to be risk factors for CRVO.…”