Serum paraoxonase activity and phenotype distribution in Turkish subjects with coronary heart disease and its relationship to serum lipids and lipoproteins

Karakaya, Asuman; İbiş, Serkan; Kural, Tuğrul; Köse, Serdal Kenan

doi:10.1016/s0009-2797(99)00085-x

Cited by 31 publications

(21 citation statements)

References 33 publications

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“…Moreover, paraoxonase activity was negatively correlated with the extent and severity of CAD. In a previous study with Turkish population, serum paraoxonase activity of patients with coronary heart disease was found to be lower than control subjects but the difference was not statistically significant (17). However, their study group was relatively small (51 controls and 57 patients) and the patients were not precisely defined whereas a fairly larger group (110 controls and 131 patients) with angiographically documented coronary artery status was enrolled in our study.…”

Section: Discussionmentioning

confidence: 74%

Relationship of PON2 gene Ser311Cys polymorphism and serum paraoxonase activity with coronary artery disease in turkish population

Bayrak¹,

Bayrak²,

Salancı³

et al. 2012

Turk J Bioch

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Objective: Paraoxonase-2 (PON2) is a cellular enzyme that has antioxidant properties similar to PON1, an HDL-associated serum enzyme playing an important role in the prevention of atherosclerosis. 1. The aim of this study is to investigate whether serum paraoxonase activity and PON2 Ser311Cys polymorphism are related to coronary artery disease and the extent of atherosclerosis. Methods: Blood specimens were collected from 131 individuals who had no coronary artery lesions angiographically and 110 individuals who had angiographically-documented coronary artery disease at several degrees. The extent and severity of arterial lesions were evaluated by Gensini scoring system. Paraoxonase and arylesterase activities were measured in serum using a spectrophotometric method. PON2 Ser311Cys polymorphism was evaluated using PCR-RFLP after DNA isolation from samples. Results: Serum paraoxonase and arylesterase activities were significantly lower in the patient group than controls (respectively, 135.6±55.4 U/ml vs 152.9±61.2 U/ml, p<0.05 and 104.7±15.6 U/ml vs 121.6±25.7 U/ml, p<0.01). There were negative correlation between serum paraoxonase, arylesterase activities and Gensini score (respectively, r=-0.183, p=0.005, r=-0.210, p=0.017). The distribution of PON2 Ser311Cys polymorphism between groups was not significant. There was no significant difference in Gensini scores according to genotypes, either. Conclusion: These results indicated that serum paraoxonase activity might be a more important marker than PON2 Ser311Cys polymorphism in evaluating the cardiovascular disease risk and the extent and severity of atherosclerosis. Key words: Serum paraoxonase activity, arylesterase activity, PON2, atherosclerosis, polymorphism Conflict of interest: The authors declared no competing interest with any group. ÖZETAmaç: Paraoksonaz 1 (PON1) aterosklerozun önlenlenmesinde önemli fonksiyonları olan, HDL ile ilişkili antioksidan bir enzimdir. PON2 ise, antioksidan özellikleri PON1'e benzeyen hücresel bir enzimdir. Bu çalışmanın amacı, serum paraoksonaz aktivitesi ve PON2 Ser311Cys polimorfizminin, koroner arter hastalığı ve ateroskleroz yaygınlığı ile ilişkisini araştırmaktır. Yöntem: Anjiyografik olarak koroner damarlarında tıkanıklık olmayan 131 birey kontrol grubu, çeşitli derecelerde tıkanıklığı olan 110 birey hasta (ateroskleroz) grubu olarak tanımlanmıştır. Aterokleroz yaygınlığı ve derecesi Gensini skoru ile değerlendirilmiştir. Paraoksonaz ve arilesteraz aktivitesi spektrofotometrik olarak ölçülmüştür. PON2 Ser311Cys polimorfizmi, örneklerden DNA izole edildikten sonra RFLP yöntemi ile incelenmiştir. Sonuçlar: Koroner arter hastalığı olan kişilerde serum paraoksonaz ve arilesteraz aktivitesi kontrol grubuna gore anlamlı derecede düşük bulunmuştur (sırasıyla, 135,6±55,4 U/ mL vs 152,9±61,2 U/mL, p<0,05 ve 104,7±15,6 U/ml vs 121,6±25,7 U/ml, p<0,01). Serum paraoksonaz, arilesteraz aktivitesi ile Gensini skoru arasında negatif korelasyon olduğu görülmüştür (r=-0,183, p=0,005 ve r=-0,210, p=0,017 ). Hasta ve kontrol grupl...

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Section: Discussionmentioning

confidence: 74%

Relationship of PON2 gene Ser311Cys polymorphism and serum paraoxonase activity with coronary artery disease in turkish population

Bayrak¹,

Bayrak²,

Salancı³

et al. 2012

Turk J Bioch

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“…Cross-sectional [27] and case-control studies [28,29] have not consistently shown an association between paraoxonase activity and various end-points of CHD, although two case-control studies yielded a positive association [26,30]. However, the smaller numbers of subjects involved in these studies may account for the contradictory results.…”

Section: Paraoxonase Activity In Cases and Controlsmentioning

confidence: 96%

Paraoxonase activity and coronary heart disease risk in healthy middle-aged males: The PRIME study

et al. 2008

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Objective: Classic coronary heart disease (CHD) risk factors fail to explain the large gradient in CHD incidence between Northern Ireland and France. The Prospective Epidemiological Study of Myocardial Infarction (PRIME) study, a multicentre prospective study of 10,593 middle-aged males, investigated novel risk factors in these populations. We tested the hypotheses that (1) higher paraoxonase activity is associated with decreased CHD risk and (2) PON55 LL genotype is associated with increased CHD risk. Methods: Paraoxonase activity was measured in 299 men who had developed CHD at 5-year follow-up and in 576 matched controls. DNA was available from 247 cases and 433 controls for genotyping for the PON55 polymorphism. Results: There was no significant difference in paraoxonase activity between cases and controls (geometric means 73.8 and 74.2 U/l; p = 0.81). There was no significant difference in CHD risk between fifths of paraoxonase activity either before (p = 0.55) or after adjustment for classical risk factors (p = 0.58).There was no significant association between genotype and CHD risk; relative to the LL genotype, the OR (95% CI) for the LM and MM genotypes were 0.92 (0.66-1.29) and 0.83 (0.50-1.36), respectively. The frequency of the L allele in cases (66.6%) and controls (64.5%) did not differ significantly, p = 0.45. Conclusions: These findings suggest that neither paraoxonase activity nor PON55 genotype is associated with CHD risk in males in the PRIME study.

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“…6,17,18,22,[26][27][28] In the present study, the evaluation of PON192 genotype distribution between healthy controls and CAD patients did not show any significant difference PON192 polymorphism in Turkish populations also showed no significant difference in the distribution of PON192 genotypes or alleles between CAD patients and controls. 29,30 PON is located on the surface of apoprotein-AI and J containing HDL-C and has a pivotal role in preventing LDL-C oxidation. 31,32 PON polymorphisms were suggested to be associated with plasma lipid levels and therefore with CAD.…”

Section: Discussionmentioning

confidence: 99%

Distribution of Pon L/M55 and Q/R192 Genotypes in Turkish Patients with Angiographically-Defined Coronary Artery Disease: Effects on Serum Lipids

Kucuk¹,

Ademoğlu²,

Türkoğlu³

et al. 2013

Turkiye Klinikleri J Med Sci

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A AB BS ST TR RA AC CT T O Ob bj je ec ct ti iv ve e: : Paraoxonase 1 (PON1) was suggested to lower the risk of coronary artery disease (CAD) by preventing high-density lipoprotein-cholesterol (HDL-C) and low-density lipoprotein-cholesterol (LDL-C) oxidation during atherosclerotic process. The relation between PON1 activity and CAD has been attributed to polymorphisms in the gene coding for this enzyme. The PON1 gene has two common polymorphisms, which lead to a leucine→methionine substitution at position 55 (PON55) and a glutamine→arginine substitution at position 192 (PON192), have been defined as the molecular basis of interindividual variability in PON activity. The aim of this study was to investigate the frequency of PON55 and PON192 polymorphisms in Turkish patients with CAD and to determine whether this polymorphism was effective on plasma lipid levels. M Ma at te er ri ia al l a an nd d M Me et th h--o od ds s: : One hundred and thirty-five angiographically-defined CAD patients and 110 healthy controls were screened for the PON55 and PON192 genotypes and plasma lipids. R Re es su ul lt ts s: : The frequency of PON55 MM genotype was significantly higher in the CAD patients than in the controls. The frequencies of PON192 genotypes were similar among CAD patients and controls. Both for PON55 and PON192 genotypes the HDL-C levels of CAD patients were significantly lower than in the corresponding controls. The severity of CAD was not associated with either the PON55 or the PON192 genotype. C Co on nc cl lu us si io on n: : PON55 and PON192 genotypes are not useful markers for the estimation of either cardiovascular risk or the severity of coronary artery disease in the Turkish population.K Ke ey y W Wo or rd ds s: : Coronary disease; cholesterol, HDL; pon1 protein, human; polymorphism, genetic; aryldialkylphosphatase Ö ÖZ ZE ET T A Am ma aç ç: : Paraoksonaz 1'in (PON1) yüksek yoğunluklu lipoprotein-kolesterol (HDL-K) ve düşük yoğunluklu lipoprotein kolesterol (LDL-K)'leri oksidasyondan koruyarak koroner arter hastalığı (KAH) görülme sıklığını azalttığı ileri sürülmektedir. PON1 aktivitesi ile KAH arasındaki ilişki, bu enzimi kodlayan genin polimorfizmlerine atfedilmektedir. Bireyler arasındaki PON aktivitesi farklığına moleküler temel oluşturan, genin 55. pozisyonundaki lösin→metiyonin değişimi (PON55) ile 192. pozisiyonundaki glutamin→arginin değişimi şeklinde iki polimorfizm tanımlanmıştır. Çal-ışmamızda, koroner arter hastalığı olan Türk hastalarda PON55 and PON192 polimorfizmlerinin frekansları ile bu polimorfizmlerin plazma lipit düzeylerine etkilerinin incelenmesi amaçlanmıştır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : KAH tanısı anjiyografik olarak konulmuş 135 hastada ve 110 sağlıklı kişide PON55 and PON192 genotipleri ve plazma lipitleri incelendi. B Bu ul lg gu ul la ar r: : Hastalarda PON55 MM genotipinin frekansının kontrollere göre anlamlı olarak daha fazla olduğu saptandı. Hasta ve kontrol grubu arasında PON192 genotiplerinin dağılımı açısından anlamlı bir fark bulunmadı. Hem PON55...

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Serum paraoxonase activity and phenotype distribution in Turkish subjects with coronary heart disease and its relationship to serum lipids and lipoproteins

Cited by 31 publications

References 33 publications

Relationship of PON2 gene Ser311Cys polymorphism and serum paraoxonase activity with coronary artery disease in turkish population

Relationship of PON2 gene Ser311Cys polymorphism and serum paraoxonase activity with coronary artery disease in turkish population

Paraoxonase activity and coronary heart disease risk in healthy middle-aged males: The PRIME study

Distribution of Pon L/M55 and Q/R192 Genotypes in Turkish Patients with Angiographically-Defined Coronary Artery Disease: Effects on Serum Lipids

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