Serum Paraoxonase (a High-density Lipoprotein-associated Lipophilic Antioxidant) Activity and Lipid Profile in Experimental Acute Pancreatitis

Unal, Ethem; Uzun, Hafize; Kuşaslan, Ramazan; Doğan, Mustafa; Genc, Habibe; Güneş, Pembegül; Titiz, İzzet

doi:10.1097/01.mpa.0000168227.74203.e4

Cited by 23 publications

(28 citation statements)

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“…In this regard, free radicals and oxidative stress have been implicated in the pathogenesis of acute pancreatitis and have been shown to correlate with the severity of pancreatitis [30,31]. Consistent with previous reports on critically ill patients [32-34], we showed that the levels of HDL and APO A-I at admission to ICU were significantly lower in non-survivors.…”

Section: Discussionsupporting

confidence: 92%

Serum levels of apolipoprotein A-I and high-density lipoprotein can predict organ failure in acute pancreatitis

et al. 2015

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IntroductionPredicting severity of pancreatitis is an important goal. Clinicians are still searching for novel and simple biomarkers that can better predict persistent organ failure (OF). Lipoproteins, especially high-density lipoprotein (HDL), and apolipoprotein A-I (APO A-I), have been shown to have anti-inflammation effects in various clinical settings. Severe acute pancreatitis (SAP) is associated with hypo-lipoproteinemia. We studied whether the concentrations of HDL and APO A-I can predict persistent OF in patients with predicted SAP admitted to the ICU.MethodsIn 66 patients with predicted SAP, we prospectively evaluated the relationship between lipid levels, inflammatory cytokines and clinical outcomes, including persistent OF and hospital mortality. Blood samples were obtained within 24 hours of admission to the ICU.ResultsHDL and APO A-I levels were inversely correlated with various disease severity scores. Patients with persistent OF had lower levels of HDL and APO A-I, while those with transient OF had lower levels of interleukin-6, tumor necrosis factor-α and lower rates of hospital mortality. Meanwhile, hospital non-survivors had lower concentrations of HDL, and APO A-I compared to the survivors. By using the area under the receiver operating characteristic (AUROC) curve, both HDL and APO A-I demonstrated an excellent discriminative power for predicting persistent OF among all patients (AUROC 0.912 and 0.898 respectively) and among those with OF (AUROC 0.904 and 0.895 respectively). Pair-wise comparison of AUROC showed that both HDL and APO A-I had better discriminative power than C-reactive protein to predict persistent OF.ConclusionsSerum levels of HDL and APO A-I at admission to the ICU are inversely correlated with disease severity in patients with predicted SAP and can predict persistent OF in this clinical setting.Electronic supplementary materialThe online version of this article (doi:10.1186/s13054-015-0832-x) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 92%

Serum levels of apolipoprotein A-I and high-density lipoprotein can predict organ failure in acute pancreatitis

et al. 2015

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“…Jung et al [36] reported that plasma viscosity mainly depends on plasma fibrinogen, albumin, globulin, and lipids levels. It has also been shown that cholesterol levels were increased in alcohol-induced acute pancreatitis [4]. Hematocrit and erythrocyte count determine the viscosity of whole blood.…”

Section: Discussionmentioning

confidence: 99%

“…It was reported in an animal study that the mortality from alcohol-induced pancreatitis was caused by a transient increase of pancreatic amylase output and plasma cholecystokinin (CCK) levels [2]. As ethanol is initially metabolized by the microsomal ethanol oxidizing system, creating an oxidative environment, oxidative stress appears to be the initial factor in the pathogenesis of alcoholic pancreatitis [1][2][3][4]. However, the detailed mechanism of induction of alcoholic pancreatitis is still not well understood.…”

Section: Introductionmentioning

confidence: 97%

Relationships among Iron, Protein Oxidation and Lipid Peroxidation Levels in Rats with Alcohol-induced Acute Pancreatitis

Kiziler

Aydemir

Gülyaşar

et al. 2008

Biol Trace Elem Res

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It has been previously shown that alcohol induces the damage of pancreatic parenchyma tissue, but the mechanism of this damage is still poorly understood. Assuming that oxygen radical damage may be the involved, we measured markers of oxidative damage in pancreatic tissue, blood serum, plasma, and whole blood of rats with early-stage alcohol-induced acute pancreatitis. Thirty-eight male Wistar rats were divided into three groups: the control group (group 1), the acute pancreatitis group 1 day (group 2), and 3 days (group 3) after the injection of ethyl alcohol into the common biliary duct, respectively. The levels of Fe in tissue and serum, whole blood viscosity, plasma viscosity, fibrinogen and homocysteine (Hcy) levels, erythrocyte and plasma malondialdehyde (MDA), and tissue and plasma protein carbonyl levels were found to be significantly higher in groups 2 and 3 than in group 1. However, the levels of reduced glutathione (GSH) in tissue and erythrocytes were significantly lower in groups 2 and 3 than in group 1. These results suggest that elevated Fe levels in serum and pancreatic tissue in rats with early-stage alcohol-induced acute pancreatitis is associated with various hemorheological changes and with oxidative damage of the pancreas.

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“…16 In this sense, a recent study focusing on acute pancreatitis has reported that PON1 activity and HDL level were lower in acute pancreatitis, whereas the mean levels of total cholesterol, low-density lipoproteins, and malonyldialdehyde were significantly higher. 17 A decrease in PON1 activity could be related with changes in liver expression, but also with the increase in oxidative stress. As what occurs with other antioxidant enzymes, such as superoxide dismutase, the inhibitory action of PON1 has been shown to be associated to an inactivation reaction that results in the lost of the functional active sites in the enzyme.…”

Section: Introductionmentioning

confidence: 98%

Serum Paraoxonase Undergoes Inhibition and Proteolysis During Experimental Acute Pancreatitis

Franco-Pons

Marsillach

Joven

et al. 2008

Journal of Gastrointestinal Surgery

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Oxidative stress has a primary role in the pathogenesis of severe acute pancreatitis. Then, the antioxidant capacity is a critical factor in the progression of this disease. Serum paraoxonase-1 (PON1) is an esterase associated with high-density lipoprotein, which clinical interest resides in its ability to prevent or limit the lipid oxidation. The aim of this study was to investigate changes in PON1 activity in the early stages of acute pancreatitis and to find out if its alteration is related with the severity of the disease. To this purpose, we used an experimental model of taurocholate-induced mild and severe acute pancreatitis. Our results showed that serum activity and PON1 concentration decreased 18 h after the induction of a severe acute pancreatitis. In vitro analysis revealed that incubation with oxidized lipids obtained from pancreatitis samples results in the inactivation of the enzyme in a concentration-dependent manner. In addition to oxidative inactivation, we observed by Western blot, an immunoreactive band suggestive of proteolytic degradation of the enzyme, altogether indicating that during severe acute pancreatitis, there is a significant decrease in serum PON1 activity. This decrease is related with inactivation of the enzyme by oxidized lipids, probably followed by proteolytic degradation of the enzyme.

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Serum Paraoxonase (a High-density Lipoprotein-associated Lipophilic Antioxidant) Activity and Lipid Profile in Experimental Acute Pancreatitis

Abstract: Our results suggest that an abnormal lipid profile and decreased PON1 activity may have a role in the pathogenesis of acute pancreatitis in which HDL-associated antioxidant defense is impaired.

Cited by 23 publications

References 13 publications

Serum levels of apolipoprotein A-I and high-density lipoprotein can predict organ failure in acute pancreatitis

Serum levels of apolipoprotein A-I and high-density lipoprotein can predict organ failure in acute pancreatitis

Relationships among Iron, Protein Oxidation and Lipid Peroxidation Levels in Rats with Alcohol-induced Acute Pancreatitis

Serum Paraoxonase Undergoes Inhibition and Proteolysis During Experimental Acute Pancreatitis

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