Serum oxidized low‐density lipoprotein is inversely correlated to telomerase activity in peripheral blood mononuclear cells of haemodialysis patients

Tsirpanlis, George; Chatzipanagiotou, Stylianos; Boufidou, Fotini; Kordinas, Vasileios; Zoga, Margarita; Alevyzaki, Fotini; Stamatelou, Kyriaki; Frangou, Eleni; Savva, Lefkothea; Nicolaou, Chryssoula

doi:10.1111/j.1440-1797.2006.00697.x

Cited by 27 publications

(16 citation statements)

References 19 publications

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“…Here we demonstrated that both telomerase activity and expression are affected in patients with a decline in renal function, a fact that might be associated with the increased inflammation markers. These conclusions concur with the results of two previous studies conducted in our laboratory [34,35]. In addition one cannot overlook the negative statistical relationship between the rise in IL-6 serum concentration and the decrease in telomerase activity.…”

Section: Discussionsupporting

confidence: 93%

Is there a connection between inflammation, telomerase activity and the transcriptional status of telomerase reverse transcriptase in renal failure?

Kordinas

Tsirpanlis

Nicolaou

et al. 2015

Cellular and Molecular Biology Letters

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Telomerase is involved in the elongation of telomeres. It remains active in very few types of cell in mature organisms. One such cell type is the lymphocytes. In this study, we investigated the activity and expression of telomerase in lymphocytes from renal failure patients and compared it to that for normal controls. Inflammation status was determined at the same time. The enzyme activity was measured using PCR-ELISA with peripheral blood mononuclear cells (PBMCs) from three groups: 53 healthy individuals, 50 patients with chronic kidney disease (CKD) and 50 dialysis patients. In the same cell populations, the expression of the reverse transcriptase of the human telomerase gene (hTERT) was measured via real-time PCR. The inflammationstatus of these individuals was determined by calculating the interleukin 6 (IL-6), IL-10, C-reactive protein (CRP) and tumor necrosis factor alpha (TNF-a) serum concentrations via ELISA. The lowest levels of telomerase activity were detected in CKD, and this group had the highest IL-6 and CRP values and the lowest hTERT expression. The dialysis group showed significant differences in comparison to the normal subjects and to the CKD patients. Further studies are warranted in order to explore the way inflammation influences telomerase activity and hTERT expression.

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Section: Discussionsupporting

confidence: 93%

Is there a connection between inflammation, telomerase activity and the transcriptional status of telomerase reverse transcriptase in renal failure?

Kordinas

Tsirpanlis

Nicolaou

et al. 2015

Cellular and Molecular Biology Letters

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“…Previous studies on haemodialysis and diabetic patients [18], [19] and patients with rheumatoid arthritis [20] have found lower PBMC telomerase activity suggesting that in a cell type like PBMC, which is directly implicated in the inflammatory process, it is generally expected a pre-mature senescence for a higher inflammatory activity [21]. On the other hand, PBMC telomerase levels measured in our study are comparable with those recently reported for overweight African Americans [22].…”

Section: Discussionsupporting

confidence: 83%

Connection between Telomerase Activity in PBMC and Markers of Inflammation and Endothelial Dysfunction in Patients with Metabolic Syndrome

et al. 2012

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Metabolic syndrome (MS) is a constellation of metabolic derangements associated with vascular endothelial dysfunction and oxidative stress and is widely regarded as an inflammatory condition, accompanied by an increased risk for cardiovascular disease. The present study tried to investigate the implications of telomerase activity with inflammation and impaired endothelial function in patients with metabolic syndrome. Telomerase activity in circulating peripheral blood mononuclear cells (PBMC), TNF-α, IL-6 and ADMA were monitored in 39 patients with MS and 20 age and sex-matched healthy volunteers. Telomerase activity in PBMC, TNF-α, IL-6 and ADMA were all significantly elevated in patients with MS compared to healthy volunteers. PBMC telomerase was negatively correlated with HDL and positively correlated with ADMA, while no association between TNF-α and IL-6 was observed. IL-6 was increasing with increasing systolic pressure both in the patients with MS and in the healthy volunteers, while smoking and diabetes were positively correlated with IL-6 only in the patients' group. In conclusion, in patients with MS characterised by a strong dyslipidemic profile and low diabetes prevalence, significant telomerase activity was detected in circulating PBMC, along with elevated markers of inflammation and endothelial dysfunction. These findings suggest a prolonged activity of inflammatory cells in the studied state of this metabolic disorder that could represent a contributory pathway in the pathogenesis of atherosclerosis.

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“…The higher concentration of oxLDL found in serum of HD patients in our study could reflect the increased LDL oxidation, and is in keeping with previous reports [26,27,28]. It has been shown that in chronic renal failure, increased LDL oxidation could be attributed to a decrease in the activity of certain enzymes with a HDL-related antioxidant effect, such as paraoxonase and lecithin-cholesterol acyltransferase (LCAT) [6,15,29,30,31].…”

Section: Discussionsupporting

confidence: 82%

Serum Oxidized LDL Is Inversely Associated with HDL2-Cholesterol Subclass in Renal Failure Patients on Hemodialysis

Samouilidou

Karpouza²,

Grapsa³

et al. 2010

Nephron Clin Pract

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Background:Dyslipoproteinemia and oxidative modification of LDL (oxLDL) are common symptoms in patients suffering from chronic renal failure on hemodialysis (HD), and contribute to the development of oxidative stress. High-density lipoprotein cholesterol (HDL-C) protects against atherosclerosis by inhibiting the oxidation of lipoproteins and by supporting reverse cholesterol transport. This study intends to examine the association of oxLDL with HDL2 and HDL3 subclasses of HDL-C in HD patients, in order to elucidate whether oxidative stress influences HDL-C composition. Methods: Thirty-four patients on HD and 21 age- and sex-matched controls were studied. HDL2 and HDL3-C subclasses were isolated from serum according to a single-step precipitation method following a homogenous HDL-C assay. oxLDL was measured by ELISA. Results: In HD patients, oxLDL concentration was higher compared to the controls (1.40 ± 0.47 vs. 0.21 ± 0.05 mg/l, p = 0.017) and was significantly associated to total cholesterol (r = 0.480, p = 0.044), LDL-C (r = 0.544, p = 0.019), HDL-C (r = –0.589, p = 0.027) and C-reactive protein (r = 0.578, p = 0.024). Comparing HDL-C subclasses, only HDL2-C was negatively correlated to oxLDL levels (r = –0.565, p = 0.035). Conclusions: In HD patients, high serum levels of oxLDL are associated with low HDL2-C subclass levels. This might suggest that oxidative stress affects the HDL subclass more related to the protecting activity of HDL-C, contributing to atherosclerosis development.

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Serum oxidized low‐density lipoprotein is inversely correlated to telomerase activity in peripheral blood mononuclear cells of haemodialysis patients

Abstract: Ox-LDL serum level is inversely correlated to telomerase activity in PBMC of HD patients. Our study proposes a new consequence of increased oxidative stress in HD patients: the premature cellular senescence potentially related to atherosclerosis through LDL oxidation.

Cited by 27 publications

References 19 publications

Is there a connection between inflammation, telomerase activity and the transcriptional status of telomerase reverse transcriptase in renal failure?

Is there a connection between inflammation, telomerase activity and the transcriptional status of telomerase reverse transcriptase in renal failure?

Connection between Telomerase Activity in PBMC and Markers of Inflammation and Endothelial Dysfunction in Patients with Metabolic Syndrome

Serum Oxidized LDL Is Inversely Associated with HDL2-Cholesterol Subclass in Renal Failure Patients on Hemodialysis

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