Serum nitric oxide metabolites in patients with multiple sclerosis

Nazlıel, Bijen; Taşkıran, Dilek; İrkeç, Ceyla; Kutay, Fatma Z.; Pöğün, Şakire

doi:10.1054/jocn.2001.1077

Cited by 28 publications

(17 citation statements)

References 22 publications

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“…Nitration of tyrosine residues by NO-derived species results in the accumulation of 3-nitrotyrosine (3 NT) in proteins, as a footprint for peroxynitrite-mediated change in the cell and as an early marker of nitrosative stress and neurodegeneration. 3 NT formation is considered to be a posttranslational modification that may be responsible for lipid peroxidation and oxidative changes in plasma proteins [3,21,24]. Acute relapses of MS are characterised by complex immunological mechanisms involving blood-brain barrier damage and influx of peripheral blood cells.…”

Section: Discussionmentioning

confidence: 99%

Original article Evaluation of oxidative and nitrosative stress in relapsing remitting multiple sclerosis: effect of corticosteroid therapy

Seven

Aslan²,

İncir³

et al. 2013

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Section: Discussionmentioning

confidence: 99%

Original article Evaluation of oxidative and nitrosative stress in relapsing remitting multiple sclerosis: effect of corticosteroid therapy

Seven

Aslan²,

İncir³

et al. 2013

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“…Reaction of these oxidized forms of nitrogen with thiol and amino groups in proteins could lead, among other things, to the formation of nitrosothiols (RSNOs) and nitrosamines (RNNOs) (15). Formation of nitrosothiols in MS has been inferred from the presence of antibodies anti-nitrosocysteine in the patients' serum and Cerebrospinal fluid (CSF) (16), and from the augmented levels of nitrite and nitrate in the blood and CSF of patients during clinical relapses (17)(18)(19)(20). Moreover, the presence of the peroxynitritite marker 3-nitrotyrosine (NT) in demyelinated plaques (9,21) supports the view that nitrosative damage is an important pathophysiological feature of MS.…”

Section: Introductionmentioning

confidence: 99%

Evidence of Nitrosative Damage in the Brain White Matter of Patients with Multiple Sclerosis

et al. 2005

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Nitric oxide (NO) has been implicated in the pathophysiology of both experimental autoimmune encephalomyelitis and multiple sclerosis (MS). NO-mediated protein damage in MS appears to be confined to large plaques where 3-nitrotyrosine has been detected. To determine whether nitrosative damage takes place beyond visible MS plaques, the occurrence of various NO-triggered protein modifications in normal-appearing white matter (NAWM) of eight MS brains was assessed and compared to that in white matter (WM) of four control brains. As determined by amino acid analysis and western blotting, no evidence of tyrosine nitration was found in the MS samples studied, suggesting that they did not contain appreciable amounts of plaque-derived material. The amino acid composition of total myelin proteins and proteolipid protein (PLP) was also unaltered in the diseased tissue, as was the fatty acid composition of PLP. In addition, we detected no changes in the number of protein free thiols suggesting that oxidation do not occur to any appreciable extent. However, the levels of nitrite in MS-NAWM were higher than those in control WM, while in the MS-gray matter (GM) the concentration of this ion was unaltered. Furthermore, five of the MS samples analyzed, and the same as those with high levels of glial fibrilary acidic protein, showed increased amounts of protein nitrosothiols as determined by the "biotin switch" method. S-nitrosation of GM proteins was again normal. There was no indication of N-nitrosation of tryptophan and N-terminal amino groups in both control and MS tissue. Overall, the data suggests that WM, but not GM, from MS brains is subjected to considerable nitrosative stress. This is the first report to present direct evidence of increased protein S-nitrosation and nitrite content in the brain parenchyma of MS patients.

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“…Cross-sectional studies considering tNOx in relation to EDSS [16,20] or disease duration [20] reported no significant relationship. A longitudinal study found higher tNOx significantly correlated with lower relapse rates over an 18 month period, supporting a prognostic role [22].…”

Section: Peripheral Bloodmentioning

confidence: 93%

Oxidative Stress-Related Biomarkers in Multiple Sclerosis: A Review

et al. 2016

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General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms AbstractObjective: To provide an up to date review of oxidative stress biomarkers in multiple sclerosis and thus identify candidate molecules with greatest promise as biomarkers of diagnosis, disease activity or prognosis.Method: A semi-systematic literature search using PubMed and other databases.Results: Nitric oxide metabolites, superoxide dismutase, catalase, glutathione reductase, inducible nitric oxide synthase, protein carbonyl, 3-nitrotyrosine, isoprostanes, malondialdehyde and products of DNA oxidation have been identified across multiple studies as having promise as diagnostic, therapeutic or prognostic markers in MS.Conclusion: Heterogeneity of study design, particularly patient selection, limits comparability across studies. Further cohort studies are needed, and we would recommend promising markers be incorporated into future clinical trials to prospectively validate their potential.

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Serum nitric oxide metabolites in patients with multiple sclerosis

Cited by 28 publications

References 22 publications

Original article Evaluation of oxidative and nitrosative stress in relapsing remitting multiple sclerosis: effect of corticosteroid therapy

Original article Evaluation of oxidative and nitrosative stress in relapsing remitting multiple sclerosis: effect of corticosteroid therapy

Evidence of Nitrosative Damage in the Brain White Matter of Patients with Multiple Sclerosis

Oxidative Stress-Related Biomarkers in Multiple Sclerosis: A Review

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