Serum miR-200c expression level as a prognostic biomarker for gastric cancer

Hp, Zhang; Fb, Sun; Sj, Li

doi:10.4238/2015.december.7.2

Cited by 20 publications

(11 citation statements)

References 28 publications

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“…The overexpression of miR-200c in a xenograft model was found to associate with a higher metastatic potential and increased metastatic colonization (32). The present findings, in which expression of miR-200c was upregulated in tumors and associated with poor prognosis and lymph node metastasis, are consistent with experimental data in ovarian (33), colorectal (34), gastric (35,36) and breast (37) cancer. The mechanism may involve miR-200c overexpression and an increase in metastatic risk by repressing the expression of E-cadherin transcriptional repressors ZEB1 and ZEB2, and the final induction of EMT.…”

Section: Discussionsupporting

confidence: 80%

Potential use of microRNA-200c as a prognostic marker in non-small cell lung cancer

Tian

Yue

et al. 2017

Oncology Letters

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Abstract. MicroRNAs (miRNAs/miRs) are a class of small, highly conserved non-coding RNAs that can serve either oncogenic or tumor-suppressive roles in a wide variety of tumors. miR-200c is a member of the miR-200 family whose specific role in non-small cell lung cancer (NSCLC) has not yet been elucidated. The purpose of the present study was to detect the expression level of miR-200c in NSCLC, and to analyze its association with clinicopathological factors and patient prognosis. The present study determined the expression levels of miR-200c in 110 tumor samples collected from patients diagnosed with NSCLC who underwent complete tumor resection with regional lymph node dissection, as assessed by reverse transcription-quantitative polymerase chain reaction. The association between the expression level of miR-200c and clinicopathological features and patient prognosis was also analyzed. The results showed that miR-200c overexpression was detected in 66 of the 110 cases and was significantly associated with positive lymph node metastasis (P<0.001). Univariate survival analysis demonstrated that high miR-200c expression, positive lymph node metastasis and advanced Tumor-Node-Metastasis (TNM) classification stage significantly predicted decreased 5-year disease-free survival rates (all P<0.05) and poor 5-year overall survival rates (all P<0.01), respectively. The results of multivariate Cox regression analysis showed that TNM stage and miR-200c expression retained its significance as an independent prognostic factor for unfavorable 5-year disease-free survival rates (P<0.05) and poor 5-year overall survival rates (P<0.01). The present findings suggest that miR-200c overexpression is significantly associated with poor survival rates in NSCLC and that miR-200c could play an oncogenic role. miR-200c may have clinical potential as a promising prognostic predictor for patients with NSCLC.

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Section: Discussionsupporting

confidence: 80%

Potential use of microRNA-200c as a prognostic marker in non-small cell lung cancer

Tian

Yue

et al. 2017

Oncology Letters

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“…This heterogeneity may influence measurements of miRNA expression, and consequently, the number of samples are tended to be interpreted as representative estimates. In addition, conflicting correlations between amounts of intracellular and extracellular (circulating) miRNAs have been observed in cancer patients, and recent studies indicate that circulating miRNAs may be potential biomarkers for malignant diseases . In fact, our study indicates the existence of multiple OSCC cell types in a single tumor mass that secrete exosomes containing a unique set of miRNAs.…”

Section: Resultsmentioning

confidence: 75%

“…Many previous reports have indicated that miR‐200c attenuates cell invasion and metastasis by downregulating the master regulators of the epithelial‐to‐mesenchymal transition (EMT)—such as ZEB1 and ZEB2—in several cancers, including head and neck cancer . In contrast, other studies propose that increased miR‐200c expression is an indicator of malignancy, as it is often found to be overexpressed in the sera of cancer patients as compared with levels in healthy controls . Le et al demonstrated the miR‐200 family‐containing extracellular vesicles are enriched in the sera of patients with metastatic cancers and ectopic expression of miR‐200 family members can enhance the metastatic ability of tumor cells in some settings via the novel miR‐200c targets TKS5 (SH3PXD2A; SH3 and PX domains 2A) and MYLK (MLCK; myosin light chain kinase).…”

Section: Resultsmentioning

confidence: 99%

miR‐200c‐3p spreads invasive capacity in human oral squamous cell carcinoma microenvironment

Kawakubo‐Yasukochi

Morioka

Hazekawa

et al. 2017

Molecular Carcinogenesis

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Oral squamous cell carcinoma (OSCC) constitutes over 90% of all cancers in the oral cavity. The prognosis for patients with invasive OSCC is poor; therefore, it is important to understand the molecular mechanisms of invasion and subsequent metastasis not only to prevent cancer progression but also to detect new therapeutic targets against OSCC. Recently, extracellular vesicles-particularly exosomes-have been recognized as intercellular communicators in the tumor microenvironment. As exosomic cargo, deregulated microRNAs (miRNAs) can shape the surrounding microenvironment in a cancer-dependent manner. Previous studies have shown inconsistent results regarding miR-200c-3p expression levels in OSCC cell lines, tissues, or serum-likely because of the heterogeneous characters of the specimen materials. For this reason, single-cell clone analyses are necessary to effectively assess the role of exosome-derived miRNAs on cells within the tumor microenvironment. The present study utilized integrated microarray profiling to compare exosome-derived miRNA and exosome-treated cell-derived mRNA expression. Data were acquired from noninvasive SQUU-A and highly invasive SQUU-B tongue cancer cell clones derived from a single patient to determine candidate miRNAs that promote OSCC invasion. Matrigel invasion assays confirmed that hsa-miR-200c-3p was a key pro-invasion factor among six miRNA candidates. Consistently, silencing of the miR-200c-3p targets, CHD9 and WRN, significantly accelerated the invasive potential of SQUU-A cells. Thus, our data indicate that miR-200c-3p in exosomes derived from a highly invasive OSCC line can induce a similar phenotype in non-invasive counterparts.

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“…Furthermore, numerous miRNAs have been determined to be associated with AD, such as miR-512 (17), miR-29c (18), and miR-155 (19). miR-613 is also reported to play a role in the pathogenesis and development of various cancers (20)(21)(22). However, there has been no data to suggest a link between miR-613 and AD.…”

Section: Discussionmentioning

confidence: 96%

MicroRNA-613 regulates the expression of brain-derived neurotrophic factor in Alzheimer's disease

Xin

et al. 2016

BST

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Serum miR-200c expression level as a prognostic biomarker for gastric cancer

Cited by 20 publications

References 28 publications

Potential use of microRNA-200c as a prognostic marker in non-small cell lung cancer

Potential use of microRNA-200c as a prognostic marker in non-small cell lung cancer

miR‐200c‐3p spreads invasive capacity in human oral squamous cell carcinoma microenvironment

MicroRNA-613 regulates the expression of brain-derived neurotrophic factor in Alzheimer's disease

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