Potential use of microRNA-200c as a prognostic marker in non-small cell lung cancer

Abstract

Abstract. MicroRNAs (miRNAs/miRs) are a class of small, highly conserved non-coding RNAs that can serve either oncogenic or tumor-suppressive roles in a wide variety of tumors. miR-200c is a member of the miR-200 family whose specific role in non-small cell lung cancer (NSCLC) has not yet been elucidated. The purpose of the present study was to detect the expression level of miR-200c in NSCLC, and to analyze its association with clinicopathological factors and patient prognosis. The present study determined the expression levels of miR-200c in 110 tumor samples collected from patients diagnosed with NSCLC who underwent complete tumor resection with regional lymph node dissection, as assessed by reverse transcription-quantitative polymerase chain reaction. The association between the expression level of miR-200c and clinicopathological features and patient prognosis was also analyzed. The results showed that miR-200c overexpression was detected in 66 of the 110 cases and was significantly associated with positive lymph node metastasis (P<0.001). Univariate survival analysis demonstrated that high miR-200c expression, positive lymph node metastasis and advanced Tumor-Node-Metastasis (TNM) classification stage significantly predicted decreased 5-year disease-free survival rates (all P<0.05) and poor 5-year overall survival rates (all P<0.01), respectively. The results of multivariate Cox regression analysis showed that TNM stage and miR-200c expression retained its significance as an independent prognostic factor for unfavorable 5-year disease-free survival rates (P<0.05) and poor 5-year overall survival rates (P<0.01). The present findings suggest that miR-200c overexpression is significantly associated with poor survival rates in NSCLC and that miR-200c could play an oncogenic role. miR-200c may have clinical potential as a promising prognostic predictor for patients with NSCLC.

miR-190, CDK1, MCM10 and NDC80 predict the prognosis of the patients with lung cancer

“…Four upregulated miRNAs and two downregulated miR- NAs (including miR-218-5p) were identified in lung adenocarcinoma (LUAD) tissues and may represent promising diagnostic and prognostic markers for patients with LUAD (29). Overexpression of miR-200c plays an oncogenic role in NSCLC and correlates markedly with adverse prognosis; thus, it may serve as a prognostic biomarker for patients with NS-CLC (30). Exosome-derived miR-302b mediates the transforming growth factor beta (TGF beta RII)/ extracellular signal-regulated kinase (ERK) pathway, which can inhibit cell proliferation and migration in LC (31).…”

Three-microRNA signature identified by bioinformatics analysis predicts prognosis of gastric cancer patients

“…At present, several miRNAs are known to be useful in the early diagnosis of cancers, including miR-21[ 19 ], miR-486[ 20 ], miR-24[ 21 ], and miR-125a-5p[ 22 ]. In addition, miR-191[ 23 ], miR-1908[ 24 ], miR-200c[ 25 ], and miR-217[ 26 ] were found to be potential prognostic indictors in cancer. However, these studies only used a single indictor or a limited number of patients for survival analysis.…”

A systematic review and meta-analysis: Association between MGMT hypermethylation and the clinicopathological characteristics of non-small-cell lung carcinoma

“…Among these two, Chinese patients have usually seen higher incidence and mortality rates of NSCLC in the last two decades 3 . Despite some advances in the therapeutic options for NSCLC in recent times, its prognosis is still very poor with a 5-year overall survival 4 . Among the many different reasons for poor outcome, epigenetic modification had played an important role in NSCLC carcinogenesis 5 , 6 .…”