Serum miR-133 as a Potential Biomarker in Acute Cerebral Infarction Patients

Xu, Peng; Jian, Xin; Li, Song; Chen, Yun; Ma, Jinfeng; Liu, Lixia; Qi, Ziyou; Pan, Xudong; Zhou, Shiqiang

doi:10.7754/clin.lab.2019.190933

Cited by 10 publications

(9 citation statements)

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“…7 IL-6, IL-8, and CRP are common inflammatory factors in ACI. 22 Hence, the contents of IL-6, IL-8, and CRP in ACI patients were detected. Pearson correlation analysis was performed between miR-124 and cerebral infarction-related inflammatory factors.…”

Section: Discussionmentioning

confidence: 92%

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miR-124 Is Downregulated in Serum of Acute Cerebral Infarct Patients and Shows Diagnostic and Prognostic Value

Zhou

2021

Clin Appl Thromb Hemost

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Acute cerebral infarct (ACI) is a severe subtype of ischemic stroke. microRNAs (miRNAs) are implicated in the pathogenesis of ACI. This study investigated the expression pattern and clinical implication of miR-124 in ACI patients. Serum samples were collected from 108 healthy people and 108 ACI patients at 24 h, 48 h, and 72 h. Serum miR-124 expression was tested using qRT-PCR. The levels of interleukin (IL)-6, IL-8, and C-reactive protein (CRP) were detected using ELISA kits. The correlations between miR-124 expression and infarct classification, infarct size, risk factors, and inflammatory factors were analyzed. The diagnostic efficacy of miR-124 in ACI was analyzed by the ROC curve. ACI patients were assigned to the miR-124 high/low expression group and the incidence of poor prognosis was compared between the two groups. miR-124 expression was poorly expressed in the serum of ACI patients. The area under the ROC curve of miR-124 in the diagnosis of ACI was 0.9527, the specificity was 91.67%, and the sensitivity was 93.52%. miR-124 expression in ACI patients was not affected by infarct classification, infarct size, low-density lipoprotein level, and homocysteine level. miR-124 expression was negatively correlated with IL-6, IL-8, and CRP in ACI patients. Low expression of miR-124 was positively correlated with the poor prognosis of ACI. miR-124 was poorly expressed in the serum of ACI patients and served as a biomarker for the diagnosis and prognosis. This study shall confer a promising novel target for the diagnosis and treatment of ACI.

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Section: Discussionmentioning

confidence: 92%

“…21 Circulating cytokines, especially the IL family, are recognized as critical mediators of inflammation. 22 CRP is an acute-phase protein, which is synthesized by hepatocytes when the body is under inflammation stimulation. 7 IL-6, IL-8, and CRP are common inflammatory factors in ACI.…”

Section: Discussionmentioning

confidence: 99%

miR-124 Is Downregulated in Serum of Acute Cerebral Infarct Patients and Shows Diagnostic and Prognostic Value

Zhou

2021

Clin Appl Thromb Hemost

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“…Significant changes in the ratio of vitamin intake were found to affect the CpG island methylation pattern in the loci of DNMT1, DNMT3A and DNMT3B, consequently leading to increased expression of the methyltransferases [ 29 ]. Even levels of miRNAs like that of miR-133 (linked to muscle and cardiac tissue metabolism and certain kinds of cancer) [ 54 , 58 , 65 , 85 , 86 , 98 , 101 ] and miR-221 (linked to oncogenesis) [ 10 , 25 , 50 , 56 , 60 , 71 , 79 , 90 , 96 , 100 ] changed in response to a skewed ratio of intake of the two vitamins. Other than sub or supra-optimal levels of folate or B12 intake, vitamin C or ascorbic acid consumption during the perinatal period also seems significant [ 19 ].…”

Section: Maternal Dietary and Supplementary Intakesmentioning

confidence: 99%

DNA methylation and regulation of gene expression: Guardian of our health

Dhar

Saha

Mitra

et al. 2021

Nucleus

103

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One of the most critical epigenetic signatures present in the genome of higher eukaryotes is the methylation of DNA at the C-5 position of the cytosine ring. Based on the sites of DNA methylation in a locus, it can serve as a repressive or activation mark for gene expression. In a crosstalk with histone modifiers, DNA methylation can consequently either inhibit binding of the transcription machinery or generate a landscape conducive for transcription. During developmental phases, the DNA methylation pattern in the genome undergoes alterations as a result of regulated balance between de novo DNA methylation and demethylation. Resultantly, differentiated cells inherit a unique DNA methylation pattern that fine tunes tissue-specific gene expression. Although apparently a stable epigenetic mark, DNA methylation is actually labile and is a complex reflection of interaction between epigenome, genome and environmental factors prior to birth and during progression of life. Recent findings indicate that levels of DNA methylation in an individual is a dynamic outcome, strongly influenced by the dietary environment during germ cell formation, embryogenesis and post birth exposures. Loss of balances in DNA methylation during developmental stages may result in imprinting disorders, while at any later stage may lead to increased predisposition to various diseases and abnormalities. This review aims to provide an outline of how our epigenome is uniquely guided by our lifetime of experiences beginning in the womb and how understanding it better holds future possibilities of improvised clinical applications.

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“…The relative level of miR-133a-3p in plasma was positively associated with IL-6, which may indicate myocardial injury. Indeed, the serum level of miR-133 was shown to be positively correlated with IL-6 in patients with the therosclerotic thrombotic cerebral infarction and cardioembolic stroke ( 88 ). In the present study, the relative expression of miR-126 was higher than in non-COVID-19 volunteers in contrast to previously published data ( 85 ).…”

Section: Discussionmentioning

confidence: 99%

Dynamic changes in radiological parameters, immune cells, selected miRNAs, and cytokine levels in peripheral blood of patients with severe COVID‑19

et al. 2023

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The present study aimed to investigate the dynamic changes in peripheral blood leucocyte subpopulations, cytokine and miRNA levels, and changes in computed tomography (CT) scores in patients with severe coronavirus disease 2019 (COVID-19) (n=14) and age-matched non-COVID-19 volunteers (n=17), which were included as a reference control group. All data were collected on the day of patient admission (day 0) and on the 7th, 14th and 28th days of follow-up while CT of the lungs was performed on weeks 2, 8, 24 and 48. On day 0, lymphopenia and leucopenia were detected in most patients with COVID-19, as well as an increase in the percentage of banded neutrophils, B cells, and CD4 + Treg cells, and a decrease in the content of PD-1 low T cells, classical, plasmacytoid, and regulatory dendritic cells. On day 7, the percentage of T and natural killer cells decreased with a concurrent increase in B cells, but returned to the initial level after treatment discharge. The content of different T and dendritic cell subsets among CD45 + cells increased during two weeks and remained elevated, suggesting the activation of an adaptive immune response. The increase of PD-1-positive subpopulations of T and non-T cells and regulatory CD4 T cells in patients with COVID-19 during the observation period suggests the development of an inflammation control mechanism. The levels of interferon γ-induced protein 10 (IP-10), tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 decreased on day 7, but increased again on days 14 and 28. C-reactive protein and granulocyte colony-stimulating factor (G-CSF) levels decreased gradually throughout the observation period. The relative expression levels of microRNA (miR)-21-5p, miR-221-3p, miR-27a-3p, miR-146a-5p, miR-133a-3p, and miR-126-3p were significantly higher at the beginning of hospitalization compared to non-COVID-19 volunteers. The plasma levels of all miRs, except for miR-126-3p, normalized within one week of treatment. At week 48, CT scores were most prominently correlated with the content of lymphocytes, senescent memory T cells, CD127 + T cells and CD57 + T cells, and increased concentrations of G-CSF, IP-10, and macrophage inflammatory protein-1α.

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Serum miR-133 as a Potential Biomarker in Acute Cerebral Infarction Patients

Cited by 10 publications

References 0 publications

miR-124 Is Downregulated in Serum of Acute Cerebral Infarct Patients and Shows Diagnostic and Prognostic Value

miR-124 Is Downregulated in Serum of Acute Cerebral Infarct Patients and Shows Diagnostic and Prognostic Value

DNA methylation and regulation of gene expression: Guardian of our health

Dynamic changes in radiological parameters, immune cells, selected miRNAs, and cytokine levels in peripheral blood of patients with severe COVID‑19

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