Serum Migration-Inhibitory Activity in Patients with Lymphoproliferative Diseases

Cohen, Stanley; Fisher, B; Yoshida, Takeshi; Bettigole, Richard E.

doi:10.1056/nejm197404182901605

Cited by 91 publications

(15 citation statements)

References 11 publications

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“…This possibility was further supported by the observation that the addition of T (39), which spontaneously synthesize DNA (40,41) and show similarities with lymphocytes detectable under conditions of known antigenic challenge (42,43). It is also consistent with the demonstration of in vivo production of migration-inhibitory lymphokinelike substances (44 …”

Section: Resultssupporting

confidence: 59%

Abnormalities of in vitro immunoglobulin synthesis by peripheral blood lymphocytes from untreated patients with Hodgkin's disease.

Romagnani¹,

Prete²,

Maggi³

et al. 1983

J. Clin. Invest.

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A B S T R A C T The immunoglobulin-synthesizing activities of peripheral blood mononuclear cells from 57 untreated patients with Hodgkin's disease and 47 normal subjects were compared. Cumulative amounts of IgM and IgG synthesized and secreted by unstimulated and pokeweed mitogen-stimulated cells over a 7-d period were determined in a solid-phase radioimmunoassay. Synthesis of IgM in unstimulated cultures and of both IgM and IgG in cultures stimulated with pokeweed mitogen was markedly reduced in patients with Hodgkin's disease, whereas the mean level of the spontaneous IgG synthesis was enhanced. The degree and frequency of in vitro abnormalities were not influenced by disease stage or histology.Depression of pokeweed mitogen-induced immunoglobulin synthesis did not correlate with excessive number of monocytes and it was unaffected by removal of phagocytic cells or addition to the cultures of monocytes from normal individuals. On the other hand, monocytes isolated from blood of patients with Hodgkin's disease were even more effective than normal monocytes in supporting pokeweed mitogen-induced immunoglobulin synthesis by normal phagocyte-depleted mononuclear cells. Synthesis of both IgM and IgG induced by pokeweed mitogen remained subnormal after addition to patient B cell cultures of autologous irradiated T cells or allogeneic normal T lymphocytes. T cells from patients with Hodgkin's disease appeared at least as effective as normal T cells in helping pokeweed mitogen-induced immunoglobulin production by normal B cells. However, when normal T cells were co-cultured with B cells from patients with Hodgkin's disease, spontaneous IgG synthesis declined, whereas the addition of patient T cells

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Section: Resultssupporting

confidence: 59%

Abnormalities of in vitro immunoglobulin synthesis by peripheral blood lymphocytes from untreated patients with Hodgkin's disease.

Romagnani¹,

Prete²,

Maggi³

et al. 1983

J. Clin. Invest.

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“…but this was not possible in three cases. The results show that the sera of 5 out of 19 patients tested were positive for MIF activity, confirming the findings of Cohen et al {1974). This number is considerably less than that found in the original study.…”

supporting

confidence: 84%

Lymphokine‐like Activity in the Serum of Patients With Haemopoietic Malignancies

Marks

Penny

1976

Aust J Exp Biol Med

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Summary Serum from, patients with haemopoietic malignancies was tested for the presence of migration inhibition factor (MIF) and leucocyte inhibition factor (LIF) activity by standard assays. There was no correlation between the specific disease state and positivity of the sera for MIF or LIF activity. MIF activity in the serum of one patient with myeloma was present in the isolated IgG cryoglobulin fraction.

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“…Lymphokines, such as migration inhibiting factor, have been found in serum, and patients with PSS have been found to have derangements of cell mediated immunity (21,22). Currie et a1 (23) reported that lymphocytes from patients with PSS were cytotoxic for cultures of fibroblasts, epithelial, or muscle cells.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of sera from patients with scleroderma

Cohen

Johnson

Hurd

1983

Arthritis & Rheumatism

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Sera from patients with progressive systemic sclerosis were compared with sera from normal individuals and from patients with other connective tissue diseases for cytotoxic effects on cultured human cells. More than 40% of the sera from patients with active progressive systemic sclerosis were cytotoxic by several criteria for pulmonary arterial or umbilical venous endothelial cells, foreskin fibroblasts, and neuroblastoma cells. Cytotoxic sera caused morphologic changes, uptake of trypan blue dye, and a decrease in the incorporation of 'H-thymidine into DNA. In contrast, only 4 sera from normal individuals or patients with other rheumatic diseases affected cell morphology, staining, or uptake of 3H-thymidine. Partial characterization of the cytotoxic factor indicated that it is sensitive to proteolysis by trypsin. The molecular weight of the factor was estimated to be similar to that of albumin.Progressive systemic sclerosis (PSS), or scleroderma, is characterized by alterations in connective tissue composition of skin and visceral organs, but the ~

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Serum Migration-Inhibitory Activity in Patients with Lymphoproliferative Diseases

Cited by 91 publications

References 11 publications

Abnormalities of in vitro immunoglobulin synthesis by peripheral blood lymphocytes from untreated patients with Hodgkin's disease.

Abnormalities of in vitro immunoglobulin synthesis by peripheral blood lymphocytes from untreated patients with Hodgkin's disease.

Lymphokine‐like Activity in the Serum of Patients With Haemopoietic Malignancies

Cytotoxicity of sera from patients with scleroderma

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