Serum microRNA in patients undergoing atrial fibrillation ablation

Kiliszek, Marek; Maciak, Karolina; Maciejak, Agata; Krzyżanowski, Krystian; Wierzbowski, Robert; Góra, Monika; Burzyńska, Beata; Segiet, Agnieszka; Skrobowski, Andrzej

doi:10.1038/s41598-020-61322-6

Cited by 15 publications

(11 citation statements)

References 21 publications

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“…However, in our TGA-RV cohort, miR-150-5p was only weakly related to worsening HF and failed to be an independent predictor what may be due to the fact that heart failure was not as severe as in the univentricular heart cohort. These findings are in line with two studies in patients with atrial fibrillation indicating miR-150 levels to be lower in patients with persistent than paroxysmal AF thus reflecting more advanced atrial remodeling (40) and miR-183 to be one of the miRNAs that may indicate recurrence of paroxysmal AF in patients after pulmonary vein isolation thus reflecting more subtle changes in atrial remodeling (41). Although TGA-RV patients carry a high atrial arrhythmic burden that was also present in our study cohort, these specific atrial arrhythmias are a different entity compared to atrial fibrillation and usually occur recurrently and a long time before the presence of overt HF.…”

Section: Discussionsupporting

confidence: 90%

MicroRNA-183-3p Is a Predictor of Worsening Heart Failure in Adult Patients With Transposition of the Great Arteries and a Systemic Right Ventricle

Abu‐Halima

Meese

Abdul‐Khaliq

et al. 2021

Front. Cardiovasc. Med.

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Aim: MicroRNAs (miRNAs) have been shown to play an important role in the progression of heart failure (HF). The aim of our study was to analyze miRNAs in the blood of patients with transposition of the great arteries and a systemic right ventricle (TGA-RV) in order to identify those that predict worsening HF.Materials and Methods: In 36 patients with TGA-RV, SurePrint™ 8 × 60K Human v21 miRNA microarrays were used to determine the miRNA abundance profiles and compared to 35 age- and gender-matched healthy volunteers (HVs). MiRNAs that were most significantly abundant or best related to worsening HF were further validated by RT-qPCR.Results: Using miRNA array analysis, a total of 50 down-regulated and 56 up-regulated miRNAs were found to be differentially abundant in TGA-RV patients compared to HVs. Six of these 106 miRNAs were significantly related to worsening HF. After validation by RT-qPCR, four miRNAs turned out to be significantly associated with worsening HF, namely miR-150-5p, miR-1255b-5p, miR-423-3p, and miR-183-3p. In the stepwise multivariable Cox regression analysis, ejection fraction of the systemic RV, high sensitive TNT and miR-183-3p were found to be independent predictors of worsening HF (P = 0.001, P = 0.002, and P = 0.001, respectively).Conclusions: In patients with TGA-RV, miR-183-3p is an independent predictor of worsening HF and thus may be used as additional biomarker in the risk assessment of these patients.

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Section: Discussionsupporting

confidence: 90%

MicroRNA-183-3p Is a Predictor of Worsening Heart Failure in Adult Patients With Transposition of the Great Arteries and a Systemic Right Ventricle

Abu‐Halima

Meese

Abdul‐Khaliq

et al. 2021

Front. Cardiovasc. Med.

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“…A previous study by Kiliszek et al performed small RNA sequencing in peripheral serum samples from AF patients undergoing catheter ablation [ 11 ]. They demonstrated 34 differentially expressed miRNAs in patients with and without AF recurrence.…”

Section: Discussionmentioning

confidence: 99%

“…However, studies that used an NGS technology to detect circulating microRNAs for AF evaluation are largely limited. A previous study by Kiliszek et al performed small RNA sequencing in peripheral serum samples from AF patients undergoing catheter ablation [11]. They demonstrated 34 differentially expressed miRNAs in patients with and without AF recurrence.…”

Section: Plos Onementioning

confidence: 99%

Circulating miR-20b-5p and miR-330-3p are novel biomarkers for progression of atrial fibrillation: Intracardiac/extracardiac plasma sample analysis by small RNA sequencing

et al. 2023

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Background Circulating microRNAs (miRNAs, miR) have been considered as biomarkers reflecting the underlying pathophysiology in atrial fibrillation (AF). Nevertheless, miRNA expression in the peripheral blood samples might not reflect a cardiac phenomenon since most miRNAs are expressed in numerous organs. This study aimed to identify the cardiac-specific circulating miRNAs as biomarkers for AF. Methods Plasma samples were obtained from a luminal coronary sinus catheter (CS, cardiac-specific samples) and femoral venous sheath (FV, peripheral samples) in patients with AF and paroxysmal supraventricular tachycardia (control, CTL) undergoing catheter ablation. The circulating miRNA profiles were analyzed by small RNA sequencing. Differently expressed miRNAs between AF and CTL were identified in each sample of the CS and FV; miRNAs exhibiting similar expression patterns in the CS and FV samples were selected as candidates for cardiac-specific biomarkers. The selected miRNAs were related to the outcome of catheter ablation of AF. Results Small RNA sequencing detected 849 miRNAs. Among the top 30 most differently expressed miRNAs between AF and CTL, circulating hsa-miR-20b-5p, hsa-miR-330-3p, and hsa-miR-204-5p had a similar pattern in the CS and FV samples. Another set of peripheral blood samples was obtained from AF patients undergoing catheter ablation (n = 141). The expression of the miR-20b-5p and miR-330-3p, but not the miR-204-5p, negatively correlated with the echocardiographic left-atrial dimension and was decreased in patients with AF recurrence as compared to those without AF recurrence during a 1-year follow-up. Conclusion Circulating miR-20b-5p and miR-330-3p can be cardiac-specific biomarkers for atrial remodeling progression and arrhythmia recurrence after catheter ablation in AF patients.

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“…In another recent study, miR-107 was investigated in AF patients undergoing catheter ablation. Although this miRNA was downregulated in sera from patients with AF recurrence compared to patients without AF recurrence, differences in expression were not significant [ 35 ]. However, the role of miR-107 in cardiovascular diseases is not well documented.…”

Section: Discussionmentioning

confidence: 99%

Pilot Study on the Role of Circulating miRNAs for the Improvement of the Predictive Ability of the 2MACE Score in Patients with Atrial Fibrillation

Rivera‐Caravaca

Teruel‐Montoya

Roldán

et al. 2020

JCM

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Background. Atrial fibrillation (AF) increases the risk for stroke but also for non-stroke major adverse cardiovascular events (MACE). The 2MACE score was recently proposed to predict these events. Since the interest of microRNAs (miRNAs) in cardiovascular diseases is increasing, we aimed to investigate whether miRNA levels may improve the predictive performance of the 2MACE score. Methods. We included consecutive AF patients stable on vitamin K antagonist therapy. Blood samples were drawn at baseline and plasma expression of miRNAs was assessed. During a median of 7.6 (interquartile range (IQR) 5.4–8.0) years, the occurrence of any MACE (nonfatal myocardial infarction/cardiac revascularization and cardiovascular death) was recorded. Results. We conducted a miRNA expression analysis in plasma from 19 patients with and without cardiovascular events. The miRNAs selected (miR-22-3p, miR-107, and miR-146a-5p) were later measured in 166 patients (47% male, median age 77 (IQR 70–81) years) and all were associated with a higher risk of MACE. The addition of miR-107 and miR-146a-5p to the 2MACE score significantly increased the predictive performance (c-indexes: 0.759 vs. 0.694, p = 0.004), and the model with three miRNAs also improved the predictive performance compared to the original score (c-indexes: 0.762 vs. 0.694, p = 0.012). 2MACE models with the addition of miRNAs presented higher net benefit and potential clinical usefulness. Conclusions. Higher miR-22-3p andmiR-107 and lower miR-146a-5p levels were associated with a higher risk of MACE. The addition of these miRNAs to the 2MACE score significantly increased the predictive performance for MACE, which may aid to some extent in the decision-making process about risk stratification in AF.

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Serum microRNA in patients undergoing atrial fibrillation ablation

Cited by 15 publications

References 21 publications

MicroRNA-183-3p Is a Predictor of Worsening Heart Failure in Adult Patients With Transposition of the Great Arteries and a Systemic Right Ventricle

MicroRNA-183-3p Is a Predictor of Worsening Heart Failure in Adult Patients With Transposition of the Great Arteries and a Systemic Right Ventricle

Circulating miR-20b-5p and miR-330-3p are novel biomarkers for progression of atrial fibrillation: Intracardiac/extracardiac plasma sample analysis by small RNA sequencing

Pilot Study on the Role of Circulating miRNAs for the Improvement of the Predictive Ability of the 2MACE Score in Patients with Atrial Fibrillation

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