Serum levels of tocopherol and retinol and their relation to triglycerides in Japanese children aged 9-12.

Hirai, Katsuyuki; Yoshida, Chise; Oshima, Hiroyuki; Murai, Y; Miyagawa, Kuniko; Takezoe, Reiko

doi:10.3164/jcbn.8.235

Cited by 7 publications

(13 citation statements)

References 30 publications

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“…As shown in Table 5, the levels of Toc and Ret in serum are correlated with the serum levels of Chol and TG. These correlations concur with our previous findings for the Nepalese in the Itahari district (24), for university students (34) and elementary school children (26) in Japan, and with those of other reports (19)(20)(21)(22). These results indicate that the changes in serum levels of Toc and Ret are closely related to the alterations in the Chol and TG levels.…”

Section: Discussionsupporting

confidence: 92%

“…It is logical that when Chol and TG levels were high, Toc and Ret were high, and that the nutritional status of Toc and Ret should not be estimated only from the serum levels. These findings suggest that the serum levels of Toc and Ret normalized to serum lipids, the ratios of Toc/Chol and Ret/Chol and the ratios of Toc/TG and Ret/TG, is a more precise way of assessing the nutritional status of Toc and Ret than the serum Toc and Ret levels only (20,22,24,26,34).…”

Section: Discussionmentioning

confidence: 93%

“…Serum vitamin E (α-and γ-tocopherol (α-Toc and γ-Toc)), vitamin A (retinol (Ret) and esterified retinol (retinyl palmitate, Ret-pal)) were extracted by hexane and separated by highperformance liquid chromatography on a Diasil 5NH 2 column (4 mm i.d.×250 mm) with isopropanol in hexane (0.8:100) as the mobile phase, as described previously (26). The amounts of Toc and Ret were measured fluorometrically (excitation, 298 nm and 340 nm; emission, 325 nm and 480 nm, respectively) and calculated from the recovery of the added internal standard of β-tocopherol for Toc and benzo(a)pyrene for Ret.…”

Section: Methodsmentioning

confidence: 99%

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Serum nutritional status of tocopherol and retinol normalized to lipids of persons living in the southern rural Terai region in Nepal

Hirai

Ohno

Jindai

et al. 2004

Environ Health Prev Med

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Objective: The present study examined the levels of serum α-Tocopherol (Toc), retinol (Ret), cholesterol (Chol) and triglycerides (TG), and their correlations in the sera of people in Nepal.Methods: The survey was conducted on the general populace in the agricultural Terai region in southern Nepal. The study population consisted of 93 males and 83 females aged 10-68 years. Serum Toc and Ret were measured by high-performance liquid chromatography.Results: No significant differences were observed between the genders for the average of total Chol (T-Chol) (140 and 145 mg/100 ml, respectively), HDL-C (45 and 47 mg/100 ml), LDL-C (94 and 97 mg/ 100 ml), and TG (106 and 110 mg/100 ml), and the ratio of LDL/HDL (2.16). The levels of mean Toc (4.32 and 4.27 μg/ml) were about the same for both genders, while the mean Ret levels were significantly higher for males (624 ng/ml) than for females (535 ng/ml) (p<0.001). A direct relationship was found between the levels of Toc and Ret (r=0.46, p<0.001 and r=0.28, p<0.05 for males and females, respectively). Serum levels of Toc and Ret were positively related to the levels of Chol (r=0.48 and r=0.58, p<0.001 for males and r=0.49, p<0.001 and r=0.33, p<0.01 for females, respectively) and TG (r=0.23 and r=0.28, p<0.05 for males and r=0.29, p<0.01 and r=0.28, p<0.05 for females, respectively). The ratio of Toc/TG normalized to serum TG was directly correlated to the ratio of Ret/TG (r=0.79 for males, and r=0.72 for females, p<0.001, respectively) and the ratios of Toc/TG and Ret/TG were negatively related to the LDL/HDL levels (r=−0.49 and r=−0.43, for males, and r=−0.46 and r=−0.57 for females, p<0.001, respectively).Conclusion: The levels of Toc and Ret were low in the sera of people living in the southern rural Terai region in Nepal, and it was found that lower levels of Toc and Ret normalized to TG increased the ratio of LDL/HDL. These results suggest that greater intake of foods rich in Toc and Ret should be encouraged to reduce the risk of coronary heart disease.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

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Serum nutritional status of tocopherol and retinol normalized to lipids of persons living in the southern rural Terai region in Nepal

Hirai

Ohno

Jindai

et al. 2004

Environ Health Prev Med

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“…The mean volumes of 24hU and 2ndU were Ret and Toc were extracted with hexane from serum by the method of Abe et al [30,31] and from urine by the method of Mitra et al for Ret [19], and Jordao et al for Toc [20], respectively, with minor modification. The extract was evaporated under nitrogen and separated by high-performance liquid chromatography (633A-F1050, Hitachi, Tokyo) on a Chromat Works, Tokyo) with isopropanol in n-hexane (0.8:100) as the mobile phase as described previously [23]. The amounts of Ret and Toc were measured fluorometrically (excitation 340 and 298 nm, emission 480 and 325 nm, respectively) and calculated from the recovery of the added internal standard and urine were examined with an autoanalyzer (Hitachi-736, Hitachi, Tokyo) using clinical kits.…”

Section: Methodsmentioning

confidence: 99%

“…These observations raised the possibility that urinary excretion of Ret and Toc may also be the case for healthy persons and their urinary excretion may play a significant role in the decrease of serum Ret and Toc. There have been reports about the serum contents of these vitamins [22][23][24][25] and their relation to food intake [26][27][28][29], but there has not been sufficient information on their urinary excretion in healthy peoples. The purpose of this study was to assess the amount of serum levels and urinary excretion of these vitamins and to clarify the relationship between their levels in urinary excretion and serum levels in young Japanese women.…”

Section: Introductionmentioning

confidence: 99%

Urinary Excretion of Retinol and Tocopherol by Young Japanese Women

Hirai

Ohno

Aoki

et al. 2004

J. Clin. Biochem. Nutr.

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Summary The urinary excretion and serum levels of retinol (Ret) and tocopherol (Toc) of 33 female university students were measured and their relationship examined. Serum and urinary levels of the kidney function parameters examined were within the normal ranges tively, and the Ret level showed a positive correlation with the level of Toc (r=0.45, p<0.01). Urinary excretion concentrations over 24 h (24hU) of Ret and Toc were 635 pg/ml tively. In urinary 2nd-spot excretion (2ndU), the concentrations were 755 pg/ml for Ret and 2.77 ng/ml for Toc and the total excretion amounts of Ret and Toc were 129 and 459 ng for the total volume of 2ndU, respectively. Serum levels of Ret and Toc were not correlated with urinary excretion in 24hU and 2ndU. The urinary excretion concentration of Ret or Toc was not statistically different between those in 24hU and 2ndU, respectively. Urinary concentration of Ret in 24hU correlated with the total amounts of Ret in 24hU, and with the urinary concentration of Ret in 2ndU (r=0.56 and r=0.57, p<0.001, respectively). Also, 24 h urinary concentration for Toc was correlated with the total amounts of Toc in 24hU, and with the urinary concentration of Toc in 2ndU (r=0.41, p<0.05 and r=0.68, p<0.001, respectively). Urinary Ret concentration of 24hU or 2ndU was correlated with the concentration of Toc excretion in the 24 h or 2nd-spot urine (r=0.52, p<0.01 and r=0.57, p<0.001, respectively). Urine excretion concentrations of Ret and Toc showed a positive correlation with the levels of urinary creatinine (r=0.73 and r=0.75, p<0.001, respectively) and urea nitrogen (r=0.53 and r=0.46, p<0.01, respectively) of the kidney function index. This study shows that the urinary excretion of Ret and Toc was independent of their serum status, and that the urinary excretion concentration and total amounts of Ret and Toc of 24hU can be estimated from those of 2ndU.

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