Serum levels of IL-8, IFNγ, IL-10, and TGF β and their gene expression levels in severe and non-severe cases of dengue virus infection

Pandey, Nidhi; Jain, Amita; Garg, Richa; Kumar, Rashmi; Agrawal, Om P.; Rao, P. Venkata

doi:10.1007/s00705-015-2410-6

Cited by 57 publications

(48 citation statements)

References 36 publications

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“…TGF‐β1 is a multifunctional cytokine involved in the several pathological process, including inflammatory events and also tissue fibrosis by regulating procoagulant and fibrinolytic activities [Marshall et al, ; Jablonska et al, ; Oshima et al, ]. TGF‐β is reported in many viral infections, such as dengue hemorrhagic fever, hepatitis C virus infection, influenza, HIV, hepatitis B virus and hantavirus infection [Li et al, ; Pandey et al, ; Sadeghi et al, ]. As in our study, TGF‐β levels reported low in severely ill patients compared to mild patients [Deng et al, ; Pandey et al, ; Sadeghi et al, ].…”

Section: Discussionsupporting

confidence: 75%

“…TGF‐β is reported in many viral infections, such as dengue hemorrhagic fever, hepatitis C virus infection, influenza, HIV, hepatitis B virus and hantavirus infection [Li et al, ; Pandey et al, ; Sadeghi et al, ]. As in our study, TGF‐β levels reported low in severely ill patients compared to mild patients [Deng et al, ; Pandey et al, ; Sadeghi et al, ]. The coagulant‐anticoagulant balance is impaired by the stimulation of endothelial cells by inflammatory factors induced by CCHF.…”

Section: Discussionsupporting

confidence: 67%

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The prognostic significance of serum TGF‐β1 levels in patients with Crimean‐Congo hemorrhagic fever

Yılmaz

Arslan

et al. 2016

Journal of Medical Virology

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Crimean-Congo Hemorrhagic Fever (CCHF) may exhibit a mild clinical course or a severe profile like mortal bleeding. The pathogenesis of the illness and reason of bleeding are unclear. However, endothelial injury is a key factor in the pathogenesis of the illness. Transforming growth factor beta (TGF-β) is one of the materials involved in repairing injured endothelium. This is a significant polypeptide released in pretty much all cells and important for the regulation of cellular events, epithelium formation, inflammation, blood coagulation, and collagen synthesis. This study aimed to determine the prognostic significance of serum TGF-β1 levels in CCHF patients. We examined 120 patients hospitalized with CCHF diagnosis and their serum TGF-β1 was investigated, retrospectively. Patients were put into two groups according to the existence of hemorrhage. Forty-four (36.7%) patients had hemorrhage. TGF-β1 levels in patients with bleeding were 5.2 ± 1.8, and 7.1 ± 2.2 for non-bleeding (P < 0.0001). When ROC analysis was performed in patients with CCHF alone in order to identify patients with bleeding, at a TGF-β1 cut-off point of 4.9, AUC was 0.762 (0.675-0.835), sensitivity 59.1%, specificity 85.5%, PPV 70.3%, and NPV 78.3%. We summarize that TGF-β1 level and endothelial dysfunction can be related. A decreased TGF-β1 level is a likely prognostic and diagnostic factor for bleeding in CCHF patients. Therefore, this marker should be considered in the treatment strategy for these patients. J. Med. Virol. 89:413-416, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionsupporting

confidence: 67%

The prognostic significance of serum TGF‐β1 levels in patients with Crimean‐Congo hemorrhagic fever

Yılmaz

Arslan

et al. 2016

Journal of Medical Virology

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“…The relationship between elevated levels of IFN-γ and dengue severity is not clear since discrepant findings have been reported. IFN-γ levels were significantly increased in serum or plasma of patients with severe dengue when compared to mild disease forms [27–29]. In contrast, Ferreira et al [30] did not detect statistical significant differences in IFN-γ levels of DHF group in comparison to dengue fever (DF) group in young Brazilian patients during the 2008 epidemic.…”

Section: Discussionmentioning

confidence: 99%

Serum from dengue virus-infected patients with and without plasma leakage differentially affects endothelial cells barrier function in vitro

et al. 2017

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BackgroundAlthough most of cases of dengue infections are asymptomatic or mild symptomatic some individuals present warning signs progressing to severe dengue in which plasma leakage is a hallmark.Methodology/Principal findingsThe present study used Electric Cell-substrate Impedance Sensing (ECIS®) which allows for electrical monitoring of cellular barrier function measuring changes in Transendothelial Electric Resistance (TEER) to investigate the parameters associated with dengue induced leakage. Three groups of individuals were tested: dengue-positives with plasma leakage (leakage), dengue-positives without plasma leakage (no leakage), and dengue-negatives (control). Data show that TEER values of human umbilical vein endothelial cells (HUVECs) was significantly lower after incubation with serum from subjects of the leakage group in comparison to the no leakage or control groups. The serum levels of CXCL1, EGF, eotaxin, IFN-γ, sCD40L, and platelets were significantly decreased in the leakage group, while IL-10, IL-6, and IP-10 levels were significantly increased. We also found a strong correlation between TEER values and augmented levels of IP-10, GM-CSF, IL-1α, and IL-8, as well as decreased levels of CXCL1 and platelets.Conclusions/SignificanceThe present work shows that the magnitude of the immune response contributes to the adverse plasma leakage outcomes in patients and that serum components are important mediators of changes in endothelial homeostasis during dengue infections. In particular, the increased levels of IP-10 and the decreased levels of CXCL1 and platelets seem to play a significant role in the disruption of vascular endothelium associated with leakage outcomes after DENV infection. These findings may have important implications for both diagnostic and therapeutic approaches to predict and mitigate vascular permeabilization in those experiencing the most severe clinical disease outcomes after dengue infection.

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“…We know that cytokines are associated with severe dengue disease (Oliveira et al, 2017;Pandey et al, 2015;Eppy et al, 2016;Tsai et al, 2014). Studies have shown high levels of IL-6 in dengue infected liver sections and elevated IL-10 levels in patients with severe dengue (Oliveira et al, 2017;Pandey et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…We know that cytokines play a crucial role in the pathogenesis of dengue, which leads to severe dengue disease (Oliveira et al, 2017;Pandey et al, 2015). To further understand the kinetics of the immune response to DENV2 with respect to secretion of cytokines, we detected the serum cytokine levels in DENV2 patient at different days after diagnosis.…”

Section: Cxcr5 + Cd8 + T Cells Were Negatively Association With Denv2mentioning

confidence: 99%

Protective role of follicular CXCR5+CD8+ T cells against dengue virus 2 infection

Qiu¹,

Wang²,

Yu³

et al. 2019

International Journal of Infectious Diseases

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Follicular CXCR5 + CD8 + T cells have antiviral effects in chronic virus infection, but the roles of these cells during dengue virus 2 (DENV2) infection remain poorly understood. Objective: This study was conducted to analyzed in detail the dynamic changes and functional properties of circulating follicular CXCR5 + CD8 + T cells to explore their effects on DENV2 infection. Methods: Circulating follicular CXCR5 + CD8 + T cells and cytokines were analyzed by flow cytometry in DENV2 patients at difference days after DENV2 infection. CD8 + T cells were isolated and purified from DENV2 patients, then were stimulated with NS1 peptides and TCR stimulant. After cultivation, multiple parameters were tested. Results: (1) CXCR5 + CD8 + T cells emerged after DENV2 infection, with high PD-1 expression, and were correlated with the reduction in DENV2 RNA viral loads. (2) PD-1 + CXCR5 + CD8 + T cells were negatively associated with disease progression. (3) Serum IFN-g, IL-6 and IL-10 levels were increased late in the course of DENV2 infection. (4) CXCR5 + CD8 + T cells from DENV2 patients exhibited increased cytotoxicity and IFN-g and IL-10 secretion. Conclusion: CXCR5 + CD8 + T cells could play a protective role in dengue pathogenesis and may be a novel strategy for controlling DENV2 infection and vaccine development.

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Serum levels of IL-8, IFNγ, IL-10, and TGF β and their gene expression levels in severe and non-severe cases of dengue virus infection

Cited by 57 publications

References 36 publications

The prognostic significance of serum TGF‐β1 levels in patients with Crimean‐Congo hemorrhagic fever

The prognostic significance of serum TGF‐β1 levels in patients with Crimean‐Congo hemorrhagic fever

Serum from dengue virus-infected patients with and without plasma leakage differentially affects endothelial cells barrier function in vitro

Protective role of follicular CXCR5+CD8+ T cells against dengue virus 2 infection

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