Protective role of follicular CXCR5+CD8+ T cells against dengue virus 2 infection

Abstract

Follicular CXCR5 + CD8 + T cells have antiviral effects in chronic virus infection, but the roles of these cells during dengue virus 2 (DENV2) infection remain poorly understood. Objective: This study was conducted to analyzed in detail the dynamic changes and functional properties of circulating follicular CXCR5 + CD8 + T cells to explore their effects on DENV2 infection. Methods: Circulating follicular CXCR5 + CD8 + T cells and cytokines were analyzed by flow cytometry in DENV2 patients at difference days after DENV2 infection. CD8 + T cells were isolated and purified from DENV2 patients, then were stimulated with NS1 peptides and TCR stimulant. After cultivation, multiple parameters were tested. Results: (1) CXCR5 + CD8 + T cells emerged after DENV2 infection, with high PD-1 expression, and were correlated with the reduction in DENV2 RNA viral loads. (2) PD-1 + CXCR5 + CD8 + T cells were negatively associated with disease progression. (3) Serum IFN-g, IL-6 and IL-10 levels were increased late in the course of DENV2 infection. (4) CXCR5 + CD8 + T cells from DENV2 patients exhibited increased cytotoxicity and IFN-g and IL-10 secretion. Conclusion: CXCR5 + CD8 + T cells could play a protective role in dengue pathogenesis and may be a novel strategy for controlling DENV2 infection and vaccine development.

COVID-19 severity associates with pulmonary redistribution of CD1c+ DCs and inflammatory transitional and nonclassical monocytes

“…In this sense, CD141 + cDCs are important for the priming of antiviral CD8 + T cell responses (15,37) and are severely depleted from the blood in all COVID-19 patients regardless of their clinical identified in the lung. CXCR5 + CD8 + T cells have been associated with control of respiratory and nonrespiratory viruses (29,38,39). In contrast, CD38 + CD8 + T cells can act as an immunosuppressive subset (40).…”

CXCR5+CD8+ T Cells: A Review of Their Antibody Regulatory Functions and Clinical Correlations

“…The Journal of Immunology complication of dengue virus 2 infection), as patients without kidney injury exhibited significantly higher quantities of CXCR5 1 CD8 1 T cells compared with those with kidney injury (66). These results were not correlated with antiviral Ab responses.…”

Role of CXCR5+CD8+ T cells in human hepatitis B virus infection

“…CXCR5 + CD8 + phenotypic T‐cells with high PD‐1 expression can also be induced after Dengue virus type 2 (DENV2) infection, and these changes are associated with a decreased DENV2 RNA load. More importantly, PD‐1 + CXCR5 + CD8 + T cells showed enhanced cytotoxicity and increased secretion of IFN‐γ and IL‐10, which were negatively correlated with disease progression 41 . Collectively, CXCR5 + CD8 + T cells contribute to the production of specific antibodies in viral infection and are associated with viral clearance, which may be a new strategy for controlling viral infection and vaccine development.…”