Serum IL‐18 levels in tuberculosis: Comparison with pneumonia, lung cancer and healthy controls

Akgün, Metin; Sağlam, Leyla; Kaynar, Hasan; Yıldırım, Abdülkadir; Mirici, Arzu; Görgüner, Metin; Meral, Mehmet; Özden, Kemalettin

doi:10.1111/j.1440-1843.2005.00710.x

Cited by 15 publications

(13 citation statements)

References 26 publications

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“…This suggests that T cell-mediated inflammatory processes remain sequestered in the lung. CXCL10 and IL-18 are recognised as important mediators of the innate immune response against mycobacteria, and elevated levels of both molecules have been detected in patients with tuberculosis (TB) [10][11][12]. Previous studies of immune function associated NTMLD with poor IFNγ responses to mitogens and/or bacterial antigens [13][14][15][16][17].…”

Section: Discussionmentioning

confidence: 99%

Immunological Markers of Lung Disease Due to Non-Tuberculous Mycobacteria

et al. 2010

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Abstract. Lung disease due to non-tuberculous mycobacteria (NTM) is a poorly understood condition that is difficult to treat. Treatment remains problematic as few tools are available to help clinicians monitor disease progression or predict treatment outcome. In this study, plasma levels of several inflammatory molecules and the frequency of circulating T cell subsets were measured in patients with NTM lung disease and known treatment status, and compared with their adult offspring and with unrelated healthy controls. Plasma levels of the chemokine CXCL10 and IL-18 were assessed for associations with treatment efficacy. CXCL10 was higher in patients than adult offspring (p < 0.001) and unrelated controls (p < 0.001). Plasma CXCL10 was also lower in patients who responded well to therapy or who controlled their infection without requiring therapy, when compared to patients who did not respond to therapy (p = 0.03). Frequencies of activated (HLA-DR + ) CD4 + T cells were higher in patients than adult offspring (p < 0.001) and unrelated controls (p < 0.05), with the highest frequencies in individuals who had completed at least 6 months of treatment. Frequencies of activated (CD38 + ) CD8 + T cells in most treatment responders were similar to unrelated controls. Low plasma levels of CXCL10 may reflect successful control of NTM lung disease with or without therapy. Compared with responders, patients who responded poorly to treatment generally had higher plasma levels of CXCL10 and IL-18, and higher frequencies of activated CD8 + T cells.

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Section: Discussionmentioning

confidence: 99%

Immunological Markers of Lung Disease Due to Non-Tuberculous Mycobacteria

et al. 2010

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“…Since gp130 is detected in suction blister fluid of control individuals, upregulated gp130 concentrations in sclerotic skin are expected to be detected in suction blister fluid in an earlier stadium than in serum. [84], gastric cancer [85], HIV related [86], kidney related [87,88], ovarian cancer [89], psoriasis [90], graft-versus-host disease [91], breast cancer related [92], cardiovascular related [93], lung disease related [94] 4/0…”

Section: Interleukin-6 Signal Transducer (Gp130)mentioning

confidence: 99%

Suction blister fluid as potential body fluid for biomarker proteins

et al. 2007

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Early diagnosis is important for effective disease management. Measurement of biomarkers present at the local level of the skin could be advantageous in facilitating the diagnostic process. The analysis of the proteome of suction blister fluid, representative for the interstitial fluid of the skin, is therefore a desirable first step in the search for potential biomarkers involved in biological pathways of particular diseases. Here, we describe a global analysis of the suction blister fluid proteome as potential body fluid for biomarker proteins. The suction blister fluid proteome was compared with a serum proteome analyzed using identical protocols. By using stringent criteria allowing less than 1% false positive identifications, we were able to detect, using identical experimental conditions and amount of starting material, 401 proteins in suction blister fluid and 240 proteins in serum. As a major result of our analysis we construct a prejudiced list of 34 proteins, relatively highly and uniquely detected in suction blister fluid as compared to serum, with established and putative characteristics as biomarkers. We conclude that suction blister fluid might potentially serve as a good alternative biomarker body fluid for diseases that involve the skin.

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“…However, IFN-γ alone is not sufficient to provide protection against disease progression [3], [4]. Other cytokines that have been found to be important in control of MTb infection include TNF-α [5], IL-12(p40) [6], IL-18 [7] and IL-17 [8]. For example, progression of LTBI to active disease can occur following TNF-α blocking treatments for chronic inflammatory diseases [5], while genetic defects involving IL-12 (as for IFN-γ [9]) increase susceptibility to development of active TB disease [6].…”

Section: Introductionmentioning

confidence: 99%

Production of TNF-α, IL-12(p40) and IL-17 Can Discriminate between Active TB Disease and Latent Infection in a West African Cohort

et al. 2010

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Background Mycobacterium tuberculosis (MTb) infects approximately 2 billion people world-wide resulting in almost 2 million deaths per year. Determining biomarkers that distinguish different stages of tuberculosis (TB) infection and disease will provide tools for more effective diagnosis and ultimately aid in the development of new vaccine candidates. The current diagnostic kits utilising production of IFN-γ in response to TB antigens can detect MTb infection but are unable to distinguish between infection and disease. The aim of this study was to assess if the use of a longer term assay and the analysis of multiple cytokines would enhance diagnosis of active TB in a TB-endemic population.MethodsWe compared production of multiple cytokines (TNF-α, IFN-γ, IL-10, IL-12(p40), IL-13, IL-17 and IL-18) following long-term (7 days) stimulation of whole-blood with TB antigens (ESAT-6/CFP-10 (EC), PPD or TB10.4) from TB cases (n = 36) and their Mycobacterium-infected (TST+; n = 20) or uninfected (TST−; n = 19) household contacts (HHC).Results and ConclusionsWe found that TNF-α production following EC stimulation and TNF-α and IL-12(p40) following TB10.4 stimulation were significantly higher from TB cases compared to TST+ HHC, while production of IFN-γ and IL-13 were significantly higher from TST+ compared to TST- HHC following PPD or EC stimulation. Combined analysis of TNF-α, IL-12(p40) and IL-17 following TB10.4 stimulation resulted in 85% correct classification into TB cases or TST+ HHC. 74% correct classification into TST+ or TST− HHC was achieved with IFN-γ alone following TB10.4 stimulation (69% following EC) and little enhancement was seen with additional cytokines. We also saw a tendency for TB cases infected with M. africanum to have increased TNF-α and IL-10 production compared to those infected with M. tuberculosis. Our results provide further insight into the pathogenesis of tuberculosis and may enhance the specificity of the currently available diagnostic tests, particularly for diagnosis of active TB.

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Serum IL‐18 levels in tuberculosis: Comparison with pneumonia, lung cancer and healthy controls

Abstract: Although increased serum levels of IL-18 were not specific for TB, the increased levels may favour active TB in radiologically advanced disease where CXR findings are difficult to interpret, and sputum smears or cultures are not helpful.

Cited by 15 publications

References 26 publications

Immunological Markers of Lung Disease Due to Non-Tuberculous Mycobacteria

Immunological Markers of Lung Disease Due to Non-Tuberculous Mycobacteria

Suction blister fluid as potential body fluid for biomarker proteins

Production of TNF-α, IL-12(p40) and IL-17 Can Discriminate between Active TB Disease and Latent Infection in a West African Cohort

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